{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:54:32Z","timestamp":1791050072355,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,1,30]],"date-time":"2009-01-30T00:00:00Z","timestamp":1233273600000},"content-version":"vor","delay-in-days":1825,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Since the roles of thromboxane A<jats:sub>2<\/jats:sub> (TXA<jats:sub>2<\/jats:sub>), prostacyclin (PGI<jats:sub>2<\/jats:sub>) and 8\u2010isoprostane F<jats:sub>2<jats:italic>\u03b1<\/jats:italic><\/jats:sub> in mediating vascular O<jats:sub>2<\/jats:sub><jats:sup>\u2022\u2212<\/jats:sup> formation and its relation to adult respiratory distress syndrome (ARDS) is unknown, the effects of these eicosanoids on the expression of gp91<jats:sup>phox<\/jats:sup> (catalytic subunit of NADPH oxidase) and O<jats:sub>2<\/jats:sub><jats:sup>\u2022\u2212<\/jats:sup> release from cultured pig pulmonary artery (PA) segments, PA vascular smooth muscle cells (PAVSMCs) and PA endothelial cells (PAECs) were investigated.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>PA segments, PAVSMCs and PAECs were incubated with the TXA<jats:sub>2<\/jats:sub> analogue, U46619, (\u00b1LPS, tumour necrosing factor\u2010alpha (TNF\u2010<jats:italic>\u03b1<\/jats:italic>) or IL\u20101<jats:italic>\u03b1<\/jats:italic>), 8\u2010isoprostane F<jats:sub>2\u03b1<\/jats:sub> and\u00b1iloprost (a stable PGI<jats:sub>2<\/jats:sub> analogue) for 16 h. The formation of superoxide dismutase\u2010inhibitable O<jats:sub>2<\/jats:sub><jats:sup>\u2022\u2212<\/jats:sup> was then measured spectrophotometrically and gp91<jats:sup>phox<\/jats:sup> expression assessed using Western blotting. In parallel experiments, whole PA segments were treated with LPS, TNF\u2010<jats:italic>\u03b1<\/jats:italic> and IL\u2010<jats:italic>\u03b1<\/jats:italic> after which time TXA<jats:sub>2<\/jats:sub>, PGI<jats:sub>2<\/jats:sub>, PGF<jats:sub>2<jats:italic>\u03b1<\/jats:italic><\/jats:sub> and 8\u2010isoprostane F<jats:sub>2<jats:italic>\u03b1<\/jats:italic><\/jats:sub> formation was measured using enzyme\u2010linked immunoassays.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>U46619, PGF<jats:sub>2<jats:italic>\u03b1<\/jats:italic><\/jats:sub> and 8\u2010isoprostane F<jats:sub>2<jats:italic>\u03b1<\/jats:italic><\/jats:sub> promoted the formation of O<jats:sub>2<\/jats:sub><jats:sup>\u2022\u2212<\/jats:sup> in PA segments, PAVSMCs and PAECs, an effect inhibited by diphenyleneiodonium and apocynin (both NADPH oxidase inhibitors) and upregulated the expression of gp91<jats:sup>phox<\/jats:sup> in PAECs and PAVSMCs. These effects were augmented by LPS, TNF\u2010<jats:italic>\u03b1<\/jats:italic> and IL\u20101<jats:italic>\u03b1<\/jats:italic> but inhibited by iloprost. Under identical incubation conditions, IL\u20101<jats:italic>\u03b1<\/jats:italic>, LPS and TNF\u2010<jats:italic>\u03b1<\/jats:italic> all induced an increase in the formation of TXA<jats:sub>2<\/jats:sub>, PGF<jats:sub>2<jats:italic>\u03b1<\/jats:italic><\/jats:sub> and 8\u2010isoprostane F<jats:sub>2<jats:italic>\u03b1<\/jats:italic><\/jats:sub> but reduced the concomitant formation of PGI<jats:sub>2<\/jats:sub>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These data demonstrate that LPS and cytokines influence the relative balance of TXA<jats:sub>2<\/jats:sub>, PGI<jats:sub>2<\/jats:sub>, PGF<jats:sub>2<jats:italic>\u03b1<\/jats:italic><\/jats:sub> and 8\u2010isoprostane F<jats:sub>2<jats:italic>\u03b1<\/jats:italic><\/jats:sub> in pig PA, which in turn alter NADPH oxidase expression and O<jats:sub>2<\/jats:sub><jats:sup>\u2022\u2212<\/jats:sup> formation. These novel findings have implications in devising effective strategies for treating ARDS.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2004) <jats:bold>141<\/jats:bold>, 488\u2013496. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0705626\">10.1038\/sj.bjp.0705626<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0705626","type":"journal-article","created":{"date-parts":[[2004,2,5]],"date-time":"2004-02-05T17:57:47Z","timestamp":1076003867000},"page":"488-496","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["Iloprost inhibits superoxide formation and gp91<sup>phox<\/sup> expression induced by the thromboxane A<sub>2<\/sub> analogue U46619, 8\u2010isoprostane F<sub>2<i>\u03b1<\/i><\/sub>, prostaglandin F<sub>2<i>\u03b1<\/i><\/sub>, cytokines and endotoxin in the pig pulmonary artery"],"prefix":"10.1111","volume":"141","author":[{"given":"Saima","family":"Muzaffar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilima","family":"Shukla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clinton","family":"Lobo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianni D","family":"Angelini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie Y","family":"Jeremy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.101.24.2833"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.98.11030745"},{"key":"e_1_2_7_4_1","first-page":"30","article-title":"Prostanoid biosynthesis in cultured rabbit renal microvascular smooth muscle cells. 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