{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T21:45:21Z","timestamp":1790459121219,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,1,30]],"date-time":"2009-01-30T00:00:00Z","timestamp":1233273600000},"content-version":"vor","delay-in-days":1825,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>In pressurised rat mesenteric small arteries (50 mmHg), we examined the effects of stimulation with U46619, endothelin\u20101 (ET\u20101) or phenylephrine (PE) on changes in vessel diameter, global [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>, individual smooth muscle cell [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and Ca<jats:sup>2+<\/jats:sup>\u2010sensitisation of contraction.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>U46619 or ET\u20101 gave tonic diameter reductions, whereas PE\u2010stimulated vessels gave tonic contractions or initial vasoconstrictions followed by diameter oscillations. Global [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> changes were transient for each agonist, with tonic constrictions being accompanied by maintained submaximal global [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> levels.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>U46619, ET\u20101 or PE tonic constrictions were accompanied by apparently asynchronous [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> waves in individual smooth muscle cells of the vessel wall, as examined by confocal fluorescent microscopy. In vessels exhibiting vasomotion to PE, some apparent synchrony of activation of individual cells was evident; however, this was incomplete with many cells responding out of phase with their neighbours.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In <jats:italic>\u03b1<\/jats:italic>\u2010toxin\u2010permeabilised preparations, agonist\u2010induced Ca<jats:sup>2+<\/jats:sup>\u2010sensitisation of constriction at submaximal Ca<jats:sup>2+<\/jats:sup> (pCa6.7) in the presence of GTP was greater with U46619 or ET than PE.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We conclude that, in pressurised mesenteric arteries, (i) a general feature of receptor\u2010coupled constriction is the generation of periodic smooth muscle [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> waves; (ii) complete synchrony of Ca<jats:sup>2+<\/jats:sup> oscillations between smooth muscle cells is not a prerequisite for receptor\u2010coupled vasomotion; (iii) varied Ca<jats:sup>2+<\/jats:sup>\u2010sensitising actions of agonists may partly determine tonic or phasic vessel responses to different stimuli.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2004) <jats:bold>141<\/jats:bold>, 678\u2013688. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0705647\">10.1038\/sj.bjp.0705647<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0705647","type":"journal-article","created":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T18:17:58Z","timestamp":1077214678000},"page":"678-688","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Comparison of U46619\u2010, endothelin\u20101\u2010 or phenylephrine\u2010induced changes in cellular Ca<sup>2+<\/sup> profiles and Ca<sup>2+<\/sup> sensitisation of constriction of pressurised rat resistance arteries"],"prefix":"10.1111","volume":"141","author":[{"given":"Linda","family":"Shaw","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"O'Neill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn J P","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clare","family":"Austin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J","family":"Taggart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,30]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"AHMED S(2001).Interactions between intracellular pH calcium and force in isolated rat portal venous smooth muscle. 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