{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:16:40Z","timestamp":1791224200589,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":1734,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The small protein Bv8, secreted by the skin of the frog <jats:italic>Bombina variegata<\/jats:italic>, belongs to a novel family of secreted proteins whose mammalian orthologues have been identified and named prokineticins (PK\u20101 and PK\u20102).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Bv8 (from 2.5 to 60 pmol) injected into the lateral ventricles of rat brain suppressed diurnal, nocturnal, deprivation\u2010induced and neuropeptide Y\u2010stimulated feeding and stimulated diurnal drinking. Nocturnal drinking was increased only in fasted rats.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>PK\u20102 mRNA is expressed in discrete areas of the rat brain, including the suprachiasmatic nucleus (SCN), medial preoptic area (MPA) and nucleus of the solitary tract (NTS). In the SCN neurons, PK\u20102 mRNA is highest during the light phase of the circadian cycle and undetectable during the dark phase.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The G\u2010protein\u2010coupled receptor prokineticin receptor 2 (PKR\u20102), which binds Bv8 and PK\u20102 with high affinity, is mainly expressed in the piriform cortex, paraventricular thalamic nucleus, parataenial nucleus (PT), SCN, hypothalamic paraventricular (PVH) and dorsomedial (DMH) nuclei, arcuate nucleus (ARC) and subfornical organ (SFO) of the rat brain.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Bv8 microinjected into the ARC, at doses from 0.02 to 2.0 pmol during night\u2010time or from 0.2 to 5 pmol in 24\u2010h\u2010fasted rats, selectively suppressed feeding without affecting drinking. When injected into the SFO, Bv8 (from 0.2 to 2 pmol) stimulated drinking but did not affect feeding. Bv8 injections into other brain areas left rat ingestive behaviours unchanged.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We hypothesize that PK\u20102\u2010rich projections from SCN neurons to PKR\u2010expressing ARC neurons could transmit the circadian rhythm of feeding, whereas inputs from the PK\u20102\u2010expressing NTS neurons to the PKR\u20102\u2010expressing SFO neurons could transmit visceral information on the water\u2013electrolyte balance and osmotic regulation.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2004) <jats:bold>142<\/jats:bold>, 181\u2013191. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0705686\">10.1038\/sj.bjp.0705686<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0705686","type":"journal-article","created":{"date-parts":[[2004,5,6]],"date-time":"2004-05-06T18:18:08Z","timestamp":1083867488000},"page":"181-191","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Bv8, the amphibian homologue of the mammalian prokineticins, modulates ingestive behaviour in rats"],"prefix":"10.1111","volume":"142","author":[{"given":"Lucia","family":"Negri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Lattanzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Giannini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena De","family":"Felice","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Colucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"Melchiorri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/35007519"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/417405a"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81084-3"},{"key":"e_1_2_7_5_1","first-page":"29","article-title":"The novel peptide Bv8 regulates fluid intake in rats","volume":"43","author":"GIANNINI E.","year":"2001","journal-title":"Pharmacological Res."},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/frne.1997.0153"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1515\/bchm2.1980.361.2.1787"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.20.1.68"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.23.10931"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903060310"},{"key":"e_1_2_7_11_1","first-page":"30","article-title":"Bv8, a small protein from frog skin induces antidiuresis in rats","volume":"43","author":"LATTANZI R.","year":"2001","journal-title":"Pharmacol. 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