{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:22:38Z","timestamp":1790713358394,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":1642,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The voltage\u2010dependent displacement of the scorpion <jats:italic>Tityus serrulatus \u03b1<\/jats:italic>\u2010toxin Ts3 was investigated in native sodium channels of GH3 cells by examining the removal of its effects in toxin\u2010free solution.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Toxin at saturating concentration was pulsed (\u223c1 s) directly onto the cell, thus causing an eight\u2010fold increase of the slow component (<jats:italic>\u03c4<\/jats:italic><jats:sub>s<\/jats:sub>=6 ms) of fast inactivation, and a three\u2010fold increase of the time constant of its fast component.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>At 0 mV, maximal conductance was achieved in cells before and after treatment with Ts3, and no displacement of the toxin could be detected.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Toxin displacement occurred if stronger depolarising pulses (&gt;100 mV) were applied. The rate of displacement depended on the amplitude and duration of the pulses, and was not related with outward Na<jats:sup>+<\/jats:sup> flux.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We propose a model in which activation does not require complete movement of segment S4 of domain IV (IVS4) and that a more extensive movement of this segment is needed for normal fast inactivation. A kinetic model is presented that can account for the typical effects of site 3 toxins.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2004) <jats:bold>142<\/jats:bold>, 1115\u20131122. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0705793\">10.1038\/sj.bjp.0705793<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0705793","type":"journal-article","created":{"date-parts":[[2004,7,29]],"date-time":"2004-07-29T20:52:18Z","timestamp":1091134338000},"page":"1115-1122","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Voltage\u2010dependent displacement of the scorpion toxin Ts3 from sodium channels and its implication on the control of inactivation"],"prefix":"10.1111","volume":"142","author":[{"given":"Fabiana V","family":"Campos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fredy I V","family":"Coronas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo S L","family":"Beir\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.70.5.567"},{"key":"e_1_2_7_3_1","first-page":"85a","article-title":"Voltage\u2010dependent dissociation of tityustoxin from sodium channels","volume":"82","author":"BEIR\u00c3O P.S.L.","year":"2002","journal-title":"Biophys. 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