{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T01:46:58Z","timestamp":1778982418291,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":1642,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>This study examines the importance of mitogen\u2010activated protein kinases (MAPKs) in upregulation of endothelin type B (ET<jats:sub>B<\/jats:sub>) receptors.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Rat middle cerebral arteries (MCAs) were incubated for 24 h with or without kinase inhibitors. Vessel segments were mounted in myographs and the contractile responses to endothelin\u20101 (ET\u20101; ET<jats:sub>A<\/jats:sub> and ET<jats:sub>B<\/jats:sub> receptor agonist) and sarafotoxin 6c (S6c; ET<jats:sub>B<\/jats:sub> receptor agonist) were studied. We used real\u2010time PCR to measure the receptor mRNA levels. An ELISA assay showed the activation of ERK1\/2 kinases after 3 h. Immunohistochemistry revealed the presence of ET<jats:sub>B<\/jats:sub> receptors on the vessels.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>After organ culture, S6c induced vasoconstriction. Incubation with the MEK\/ERK inhibitors U0126 and SB386023 diminished the contractile response to S6c. The p38 MAPK inhibitor SB239063 did not affect the S6c\u2010induced contraction.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The ET\u20101\u2010induced vasoconstriction was increased after incubation with SB386023 or SB239063, while unaffected by U0126.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The ET<jats:sub>B<\/jats:sub> receptor mRNA levels were diminished by SB386023 and U0126. The ET<jats:sub>A<\/jats:sub> receptor mRNA levels were unaffected.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The levels of activated ERK1\/2 kinases were significantly higher after 3 h of organ culture as compared to fresh vessels.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The level of ET<jats:sub>B<\/jats:sub> receptor protein on the smooth muscle cells of the MCA, visualised by immunohistochemistry, was somewhat diminished by SB386023.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Our results show that the ERK1\/2 MAPK is important in the upregulation of contractile ET<jats:sub>B<\/jats:sub> receptors in MCA after organ culture. Since there is a similar upregulation in models of focal ischaemia and subarachnoid haemorrhage, this may be an important pathophysiological event.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2004) <jats:bold>142<\/jats:bold>, 1155\u20131161. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0705803\">10.1038\/sj.bjp.0705803<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0705803","type":"journal-article","created":{"date-parts":[[2004,7,29]],"date-time":"2004-07-29T20:52:18Z","timestamp":1091134338000},"page":"1155-1161","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Importance of ERK1\/2 in upregulation of endothelin type B receptors in cerebral arteries"],"prefix":"10.1111","volume":"142","author":[{"given":"Marie","family":"Henriksson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cang\u2010Bao","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Edvinsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.22.12866"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-1128(200103)21:2<129::AID-MED1003>3.0.CO;2-H"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90098-J"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.31.2.347"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.16.4.279.10693"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.29.18623"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1227\/01.NEU.0000058467.82442.64"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000072984.79136.79"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1983.tb07178.x"},{"key":"e_1_2_7_11_1","first-page":"452","volume-title":"Cerebral Blood Flow and Metabolism","author":"LEES K.R.","year":"2002"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0404.2002.01313.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199908020-00025"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60765-4"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(95)00494-7"},{"key":"e_1_2_7_16_1","first-page":"137","article-title":"International union of pharmacology nomenclature of endothelin receptors","volume":"46","author":"MASAKI T.","year":"1994","journal-title":"Pharmacol. 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