{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:00:41Z","timestamp":1790866841607,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":1703,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>In this study, intracellular Ca<jats:sup>2+<\/jats:sup> was measured as the Fura\u20102 ratio (<jats:italic>R<\/jats:italic>) of fluorescence excited at 340 and 380 nm (<jats:italic>F<\/jats:italic><jats:sub>340<\/jats:sub>\/<jats:italic>F<\/jats:italic><jats:sub>380<\/jats:sub>) in nonpressurized rat mesenteric small arterioles (\u00d8 (lumen diameter) 10\u201325 <jats:italic>\u03bc<\/jats:italic>m). The response to depolarization using 75 m<jats:sc>M<\/jats:sc> KCl was an increase in <jats:italic>R<\/jats:italic> from a baseline of 0.96\u00b10.01 ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> \u223c74 n<jats:sc>M<\/jats:sc>) to 1.04\u00b10.01 (\u223c128 n<jats:sc>M<\/jats:sc>) (<jats:italic>n<\/jats:italic>=80).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The response to 75 m<jats:sc>M<\/jats:sc> K<jats:sup>+<\/jats:sup> was reversibly abolished in Ca<jats:sup>2+<\/jats:sup>\u2010free physiological saline solution, whereas phentolamine (10 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) or tetrodotoxin (1 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) had no effects. LaCl<jats:sub>3<\/jats:sub> (200 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) inhibited 61\u00b19% of the response.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>A [K<jats:sup>+<\/jats:sup>]\u2010response curve indicated that the Ca<jats:sup>2+<\/jats:sup> response was activated between 15 and 25 m<jats:sc>M<\/jats:sc> K<jats:sup>+<\/jats:sup>. The data suggest that the Ca<jats:sup>2+<\/jats:sup> response was caused by the activation of voltage\u2010dependent Ca<jats:sup>2+<\/jats:sup> channels.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Mibefradil use dependently inhibited the Ca<jats:sup>2+<\/jats:sup> response to 75 m<jats:sc>M<\/jats:sc> K<jats:sup>+<\/jats:sup> by 29\u00b12% (100 n<jats:sc>M<\/jats:sc>), 73\u00b17% (1 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) or 89\u00b17% (10 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>). Pimozide (500 n<jats:sc>M<\/jats:sc>) use dependently inhibited the Ca<jats:sup>2+<\/jats:sup> response by 85\u00b11%.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Nifedipine (1 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) inhibited the Ca<jats:sup>2+<\/jats:sup> response to 75 m<jats:sc>M<\/jats:sc> K<jats:sup>+<\/jats:sup> by 41\u00b112%. The response was not inhibited by calciseptine (500 n<jats:sc>M<\/jats:sc>), <jats:italic>\u03c9<\/jats:italic>\u2010agatoxin IVA (100 n<jats:sc>M<\/jats:sc>), <jats:italic>\u03c9<\/jats:italic>\u2010conotoxin MVIIA (500 n<jats:sc>M<\/jats:sc>), or SNX\u2010482 (100 n<jats:sc>M<\/jats:sc>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Using reverse transcriptase\u2013polymerase chain reaction, it was shown that neither Ca<jats:sub>V<\/jats:sub>2.1a (P\u2010type) nor Ca<jats:sub>V<\/jats:sub>2.1b (Q\u2010type) voltage\u2010dependent Ca<jats:sup>2+<\/jats:sup> channels were expressed in mesenteric arterioles, whereas the Ca<jats:sub>V<\/jats:sub>3.1 (T\u2010type) channel was expressed. Furthermore, no amplification products were detected when using specific primers for the <jats:italic>\u03b2<\/jats:italic><jats:sub>1b<\/jats:sub>, <jats:italic>\u03b2<\/jats:italic><jats:sub>2<\/jats:sub>, or <jats:italic>\u03b2<\/jats:italic><jats:sub>3<\/jats:sub> auxiliary subunits of high\u2010voltage\u2010activated Ca<jats:sup>2+<\/jats:sup> channels.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results suggest that the voltage\u2010dependent Ca<jats:sup>2+<\/jats:sup> channel activated by sustained depolarization in mesenteric arterioles does not classify as any of the high\u2010voltage\u2010activated channels (L\u2010, P\/Q\u2010, N\u2010, or R\u2010type), but is likely to be a T\u2010type channel. The possibility that the sustained Ca<jats:sup>2+<\/jats:sup> influx observed was the result of a T\u2010type window current is discussed.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2004) <jats:bold>142<\/jats:bold>, 709\u2013718. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0705841\">10.1038\/sj.bjp.0705841<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0705841","type":"journal-article","created":{"date-parts":[[2004,6,17]],"date-time":"2004-06-17T17:18:11Z","timestamp":1087492691000},"page":"709-718","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Depolarization\u2010induced calcium influx in rat mesenteric small arterioles is mediated exclusively <i>via<\/i> mibefradil\u2010sensitive calcium channels"],"prefix":"10.1111","volume":"142","author":[{"given":"Lars J","family":"Jensen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Salomonsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boye L","family":"Jensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels\u2010Henrik","family":"Holstein\u2010Rathlou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2000.279.6.F997"},{"key":"e_1_2_7_3_1","first-page":"1104","article-title":"Pharmacological properties of the T\u2010type Ca2+ current of mouse spermatogenic cells","volume":"53","author":"ARNOULT C.","year":"1998","journal-title":"Mol. 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