{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:11:55Z","timestamp":1761675115373},"reference-count":45,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":1642,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The effects of nicotinic acetylcholine receptor antagonists were studied on currents evoked by application of ATP to rat isolated dorsal root ganglion cells, and human embryonic kidney 293 cells expressing rat P2X<jats:sub>3<\/jats:sub> and P2X<jats:sub>2\/3<\/jats:sub> receptors.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The rapidly desensitising (within 100 ms) current in dorsal root ganglion cells was inhibited by methyllycaconitine, <jats:italic>\u03b1<\/jats:italic>\u2010bungarotoxin and (+)\u2010tubocurarine (concentrations giving half\u2010maximal inhibition were approximately 40, 60 and 800 n<jats:sc>M<\/jats:sc>, respectively), but not by hexamethonium (100 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) or mecamylamine (100 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>). The sustained (&gt;250 ms) current in dorsal root ganglion cells was inhibited by (+)\u2010tubocurarine (80% by 10 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>), but not by methyllycaconitine (200 n<jats:sc>M<\/jats:sc>), <jats:italic>\u03b1<\/jats:italic>\u2010bungarotoxin (200 n<jats:sc>M<\/jats:sc>), mecamylamine (100 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) or hexamethonium (100 <jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Rapidly desensitising currents evoked by <jats:italic>\u03b1<\/jats:italic>,<jats:italic>\u03b2<\/jats:italic>methylene\u2010ATP in human embryonic kidney cells expressing P2X<jats:sub>3<\/jats:sub> receptors were inhibited by methyllycaconitine and <jats:italic>\u03b1<\/jats:italic>\u2010bungarotoxin, at concentrations similar to those effective in dorsal root ganglion cells.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results indicate that some nicotinic acetylcholine receptor antagonists are potent blockers of P2X receptors on neurons, particularly the homo\u2010oligomeric P2X<jats:sub>3<\/jats:sub> receptor. This finding suggests that these drugs should be used with care to discriminate between P2X and neuronal acetylcholine receptor types.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2004) <jats:bold>142<\/jats:bold>, 1227\u20131232. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0705878\">10.1038\/sj.bjp.0705878<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0705878","type":"journal-article","created":{"date-parts":[[2004,8,12]],"date-time":"2004-08-12T12:10:14Z","timestamp":1092312614000},"page":"1227-1232","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Methyllycaconitine, <i>\u03b1<\/i>\u2010bungarotoxin and (+)\u2010tubocurarine block fast ATP\u2010gated currents in rat dorsal root ganglion cells"],"prefix":"10.1111","volume":"142","author":[{"given":"Ulyana","family":"Lalo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuri","family":"Pankratov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oleg","family":"Krishtal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Alan North","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"802","article-title":"Blockade of nicotinic currents in hippocampal neurons defines methyllycaconitine as a potent and specific receptor antagonist","volume":"41","author":"ALKONDON M.","year":"1992","journal-title":"Mol. 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