{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:34:29Z","timestamp":1791646469512,"version":"4.3.4"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":1642,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The aim of the present study was to determine whether inhibition of cyclic nucleotide phosphodiesterase (PDE) modulates the stimulated generation of the cytokines, interleukin\u20104 (IL\u20104) and IL\u201013, from human basophils. This was addressed by evaluating the effects of both nonselective and selective inhibitors of PDEs on the generation of cytokines from basophils.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The nonselective PDE inhibitors, isobutyl\u2010methylxanthine (IBMX) and theophylline, attenuated the IgE\u2010mediated generation of IL\u20104 and IL\u201013 and, also, the release of histamine from basophils.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The effects of the isoform\u2010selective inhibitors, 8\u2010methoxymethyl\u2010IBMX (PDE 1 inhibitor), siguazodan (PDE3 inhibitor), rolipram (PDE4 inhibitor), denbufylline (PDE4 inhibitor), Org 30029 (mixed PDE3 and 4 inhibitor) and zaprinast (PDE5 inhibitor), were studied. Of these selective compounds, only rolipram, denbufylline and Org 30029 inhibited the IgE\u2010dependent generation of IL\u20104, IL\u201013 and histamine from basophils to a statistically significant (<jats:italic>P<\/jats:italic>&lt;0.05) degree.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The effects of isoform\u2010selective inhibitors on basophils activated by IL\u20103 were evaluated. The IL\u20103\u2010induced generation of IL\u20104, IL\u201013 and histamine was inhibited to a statistically significant (<jats:italic>P<\/jats:italic>&lt;0.05) extent, only by compounds that act as inhibitors of PDE4.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These data suggest that inhibition of PDE4 can regulate the generation of cytokines from human basophils.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2004) <jats:bold>142<\/jats:bold>, 1265\u20131272. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0705892\">10.1038\/sj.bjp.0705892<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0705892","type":"journal-article","created":{"date-parts":[[2004,8,12]],"date-time":"2004-08-12T12:10:14Z","timestamp":1092312614000},"page":"1265-1272","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effects of phosphodiesterase inhibitors on interleukin\u20104 and interleukin\u201013 generation from human basophils"],"prefix":"10.1111","volume":"142","author":[{"given":"Nahid","family":"Eskandari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thulani","family":"Wickramasinghe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter T","family":"Peachell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"399","article-title":"Multiple cyclic nucleotide phosphodiesterases","volume":"46","author":"BEAVO J.A.","year":"1994","journal-title":"Mol. 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