{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T04:05:26Z","timestamp":1759032326607},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,2,17]],"date-time":"2009-02-17T00:00:00Z","timestamp":1234828800000},"content-version":"vor","delay-in-days":1600,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>We studied amobarbital's effects on membrane properties and currents, and electrically evoked inhibitory postsynaptic currents (IPSCs) mediated by <jats:italic>\u03b3<\/jats:italic>\u2010aminobutyric acid (GABA) in rat thalamic slices. Using concentration\u2013response relationships, we compared amobarbital's effects in nociceptive nuclei and non\u2010nociceptive nucleus reticularis thalami (nRT).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Amobarbital decreased input resistance by activating GABA<jats:sub>A<\/jats:sub> receptors. Amobarbital produced a larger decrease in ventrobasal than nRT neurons.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Amobarbital depressed burst and tonic firing. Depression of burst firing was more effective, particularly in ventrobasal and intralaminar neurons. Depression was reversed by GABA<jats:sub>A<\/jats:sub> antagonists, and surmountable by increasing current injection, implicating a receptor\u2010mediated shunt mechanism.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Amobarbital did not affect the tetrodotoxin\u2010isolated low threshold Ca<jats:sup>2+<\/jats:sup> spike during GABA<jats:sub>A<\/jats:sub> blockade. Amobarbital reduced excitability without altering outward leak, or hyperpolarisation\u2010activated inward currents.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Amobarbital increased mean conductance and burst duration of single GABA<jats:sub>A<\/jats:sub> channels. Consistent with this, amobarbital increased amplitude and decay time of IPSCs with distinct EC<jats:sub>50<\/jats:sub>s, implicating actions at two GABA<jats:sub>A<\/jats:sub> receptor sites.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Activation of GABA<jats:sub>A<\/jats:sub> receptors by low concentrations, fast IPSC amplitude modulation, and failure to affect intrinsic currents distinguished amobarbital's mechanism of action from previously characterised barbiturates. The selective actions of amobarbital on GABA<jats:sub>A<\/jats:sub> receptor may have relevance in explaining anaesthetic and analgesic uses.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p> <jats:italic>British Journal of Pharmacology<\/jats:italic> (2004) <jats:bold>143<\/jats:bold>, 485\u2013494. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0705974\">10.1038\/sj.bjp.0705974<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0705974","type":"journal-article","created":{"date-parts":[[2004,10,14]],"date-time":"2004-10-14T18:48:13Z","timestamp":1097779693000},"page":"485-494","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Selective GABA\u2010receptor actions of amobarbital on thalamic neurons"],"prefix":"10.1111","volume":"143","author":[{"given":"H\u2010S","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X","family":"Wan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D A","family":"Mathers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Puil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,17]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"497","article-title":"Interaction of barbiturate analogs with the Torpedo californica nicotinic acetylcholine receptor ion channel","volume":"60","author":"ARIAS H.R.","year":"2001","journal-title":"Mol. 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