{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T06:04:59Z","timestamp":1772172299677,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":1489,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Reticular thalamocortical neurons express a slowly inactivating T\u2010type Ca<jats:sup>2+<\/jats:sup> current that is quite similar to that recorded from recombinant Ca<jats:sub>v<\/jats:sub>3.3b (<jats:italic>\u03b1<\/jats:italic>1Ib) channels. These neurons also express abundant Ca<jats:sub>v<\/jats:sub>3.3 mRNA, suggesting that it underlies the native current.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Here, we test this hypothesis by comparing the anesthetic sensitivities of recombinant Ca<jats:sub>v<\/jats:sub>3.3b channels stably expressed in HEK 293 cells to native T channels in reticular thalamic neurons (nRT) from brain slices of young rats.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Barbiturates completely blocked both Ca<jats:sub>v<\/jats:sub>3.3 and nRT currents, with pentobarbital being about twice more potent in blocking Ca<jats:sub>v<\/jats:sub>3.3 currents. Isoflurane had about the same potency in blocking Ca<jats:sub>v<\/jats:sub>3.3 and nRT currents, but enflurane, etomidate, propofol, and ethanol exhibited 2\u20134 fold higher potency in blocking nRT vs Ca<jats:sub>v<\/jats:sub>3.3 currents.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Nitrous oxide (N<jats:sub>2<\/jats:sub>O; laughing gas) blocked completely nRT currents with IC<jats:sub>50<\/jats:sub> of 20%, but did not significantly affect Ca<jats:sub>v<\/jats:sub>3.3 currents at four\u2010fold higher concentrations. In addition, we observed that in lower concentration, N<jats:sub>2<\/jats:sub>O reversibly increased nRT but not Ca<jats:sub>v<\/jats:sub>3.3 currents.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In conclusion, contrasting anesthetic sensitivities of Ca<jats:sub>v<\/jats:sub>3.3 and nRT T\u2010type Ca<jats:sup>2+<\/jats:sup> channels strongly suggest that different molecular structures of Ca<jats:sup>2+<\/jats:sup> channels give rise to slowly inactivating T\u2010type Ca<jats:sup>2+<\/jats:sup> currents. Furthermore, effects of volatile anesthetics and ethanol on slowly inactivating T\u2010type Ca<jats:sup>2+<\/jats:sup> channel variants may contribute to the clinical effects of these agents.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2005) <jats:bold>144<\/jats:bold>, 59\u201370. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0706020\">10.1038\/sj.bjp.0706020<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0706020","type":"journal-article","created":{"date-parts":[[2004,12,7]],"date-time":"2004-12-07T01:12:55Z","timestamp":1102381975000},"page":"59-70","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Contrasting anesthetic sensitivities of T\u2010type Ca<sup>2+<\/sup> channels of reticular thalamic neurons and recombinant Ca<sub>v<\/sub>3.3 channels"],"prefix":"10.1111","volume":"144","author":[{"given":"Pavle M","family":"Joksovic","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara C","family":"Brimelow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet","family":"Murbarti\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward","family":"Perez\u2010Reyes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Slobodan M","family":"Todorovic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"871","volume-title":"Clinical Anesthesia","author":"BARASH P.G.","year":"1992"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-199102000-00022"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02741375"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.013269"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.83.1.103"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112355"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/367607a0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1991.tb33852.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1992.68.1.213"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.58.030196.001553"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1993.70.1.158"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.12-10-03804.1992"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0498-460"},{"key":"e_1_2_7_15_1","first-page":"271a","article-title":"Volatile anesthetics block T\u2010type Ca2+ channels in nucleus reticularis thalami in brain slices","volume":"86","author":"JOKSOVIC P.M.","year":"2004","journal-title":"Biophys. J."},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)01401-8"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.1999.00841.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-06-01912.1999"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp017288"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-198110000-00005"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-199611000-00026"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.38.1.201"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(99)00319-5"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.103.053272"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)03341-0"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(98)00097-5"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-547620-1.50007-2"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00018.2002"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/36110"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.13.7760"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(97)00086-5"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1999.81.4.1795"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-199407000-00016"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.11-05-01404.1991"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-06-01895.1999"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1988.tb11553.x"},{"key":"e_1_2_7_37_1","first-page":"603","article-title":"Ca(v)3.2 channel is a molecular substrate for inhibition of T\u2010type calcium currents in rat sensory neurons by nitrous oxide","volume":"60","author":"TODOROVIC S.M.","year":"2001","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1998.79.1.240"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.58.1.98"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-0186(99)00050-9"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0706020","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0706020","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0706020","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T03:05:29Z","timestamp":1697166329000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0706020"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,1]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,1]]}},"alternative-id":["10.1038\/sj.bjp.0706020"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0706020","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,1]]},"assertion":[{"value":"2009-01-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}