{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T05:19:18Z","timestamp":1767849558164,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":1458,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The pharmacology of bimatoprost, a synthetic prostaglandin\u2010amide, was examined in prostaglandin F<jats:sub>2<jats:italic>\u03b1<\/jats:italic><\/jats:sub> (PGF<jats:sub>2<jats:italic>\u03b1<\/jats:italic><\/jats:sub>)\u2010sensitive preparations. Bimatoprost potently contracted the rabbit isolated uterus (pEC<jats:sub>50<\/jats:sub>=7.92\u00b10.16). In contrast, bimatoprost exhibited weak excitatory activity in human myometrium from pregnant and nonpregnant donors, mouse uterus, rat uterus, and endothelium\u2010intact rabbit jugular veins, and did not stimulate DNA synthesis in mouse fibroblasts.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The possibility that the effects of bimatoprost may reflect partial agonism at prostanoid FP receptors was examined and the contractile effects of full agonists, 17\u2010phenyl PGF<jats:sub>2<jats:italic>\u03b1<\/jats:italic><\/jats:sub> (FP) and U\u201046619 (TP, a control), were determined in the absence and presence of 1\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc> bimatoprost on the mouse uterus. Analyses of the agonist\u2013agonist functional studies showed no antagonism, indicating that bimatoprost is not a partial agonist.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Bioassay metabolism studies of bimatoprost and latanoprost (FP receptor agonist prodrug) in the rabbit uterus were conducted using recipient mouse uterus. Results indicated that the potent responses to bimatoprost in the rabbit uterus are produced by the intact molecule and not by its putative free acid metabolite, 17\u2010phenyl PGF<jats:sub>2<jats:italic>\u03b1<\/jats:italic><\/jats:sub>. Some hydrolysis of latanoprost to latanoprost free acid appears to have occurred in the rabbit uterus, according to biological detection.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The pharmacology of bimatoprost could not be explained by its interaction with known prostanoid FP receptors and was independent of species\u2010, tissue\u2010, or preparation\u2010related factors. The potent contractile effects of bimatoprost in the rabbit uterus provide further pharmacological evidence for the presence of a novel receptor population that preferentially recognises bimatoprost.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2005) <jats:bold>144<\/jats:bold>, 493\u2013501. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0706044\">10.1038\/sj.bjp.0706044<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0706044","type":"journal-article","created":{"date-parts":[[2005,1,27]],"date-time":"2005-01-27T16:01:08Z","timestamp":1106841668000},"page":"493-501","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Studies using isolated uterine and other preparations show bimatoprost and prostanoid FP agonists have different activity profiles"],"prefix":"10.1111","volume":"144","author":[{"given":"June","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judith","family":"Senior","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kay","family":"Marshall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farhat","family":"Abbas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen","family":"Dinh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Dinh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larry","family":"Wheeler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Woodward","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"119","volume-title":"Molecular Pharmacology: The Mode of Action of Biologically Active Compounds","author":"ARI\u00cbNS E.J.","year":"1964"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0952-3278(94)90139-2"},{"key":"e_1_2_7_4_1","first-page":"163","article-title":"Determination of DNA concentration with diphenylamine","volume":"23","author":"BURTON K.","year":"1979","journal-title":"Methods Enzymol."},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb15960.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-6980(98)00034-3"},{"key":"e_1_2_7_7_1","volume-title":"Current Protocols in Pharmacology","author":"CHEN J.","year":"2001"},{"key":"e_1_2_7_8_1","first-page":"95","article-title":"Lumigan\u00ae: a novel drug for glaucoma therapy","volume":"3","author":"CHEN J.","year":"2002","journal-title":"Optometry Pract."},{"key":"e_1_2_7_9_1","first-page":"229","volume-title":"The IUPHAR Compendium of Receptor Characterization and Classification","author":"COLEMAN R.A.","year":"1998"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/108076803762718105"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1081\/RRS-100107425"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(02)01891-2"},{"key":"e_1_2_7_13_1","first-page":"202P","article-title":"Rabbit jugular vein contains three different relaxant prostaglandin receptors","volume":"99","author":"GILES H.","year":"1990","journal-title":"Br. 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