{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:57:16Z","timestamp":1777427836874,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":1246,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2005,9]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>We have investigated whether the activation of cAMP\u2010 and cGMP\u2010dependent pathways modifies the properties of voltage\u2010dependent Ba<jats:sup>2+<\/jats:sup> currents (<jats:italic>I<\/jats:italic><jats:sub>Ba<\/jats:sub>) recorded from guinea\u2010pig gastric myocytes using patch\u2010clamp techniques. All experiments were carried on single smooth muscle cells, dispersed from the circular layer of the guinea\u2010pig gastric antrum.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Both dibutyryl cAMP (db\u2010cAMP, 0.1\u20131\u2003m<jats:sc>M<\/jats:sc>), a membrane\u2010permeable ester of cAMP, and isoproterenol, a selective <jats:italic>\u03b2<\/jats:italic>\u2010stimulant, inhibited <jats:italic>I<\/jats:italic><jats:sub>Ba<\/jats:sub> in a concentration\u2010dependent manner.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Forskolin, but not dideoxy\u2010forskolin, an inactive isomer of forskolin, inhibited the peak amplitude of <jats:italic>I<\/jats:italic><jats:sub>Ba<\/jats:sub>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In the presence of either Rp\u2010cAMP or the PKA (cAMP\u2010dependent protein kinase) inhibitor peptide 5\u201024 (PKA\u2010IP), neither forskolin nor db\u2010cAMP inhibited <jats:italic>I<\/jats:italic><jats:sub>Ba<\/jats:sub>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>After establishing a conventional whole\u2010cell recording, the peak amplitude of <jats:italic>I<\/jats:italic><jats:sub>Ba<\/jats:sub> gradually decreased when the catalytic subunit of PKA was included in the pipette. The further application of Rp\u2010cAMP reversibly enhanced <jats:italic>I<\/jats:italic><jats:sub>Ba<\/jats:sub>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Sodium nitroprusside (0.1\u20131\u2003m<jats:sc>M<\/jats:sc>) and 8\u2010Br\u2010cGMP (0.1\u20131\u2003m<jats:sc>M<\/jats:sc>) also inhibited <jats:italic>I<\/jats:italic><jats:sub>Ba<\/jats:sub> in a concentration\u2010dependent manner.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The inhibitory effects of forskolin or db\u2010cAMP on <jats:italic>I<\/jats:italic><jats:sub>Ba<\/jats:sub> were not significantly changed by pretreatment with a cGMP\u2010dependent protein kinase (PKG) inhibitor. Similarly, the inhibitory actions of 8\u2010Br\u2010cGMP on <jats:italic>I<\/jats:italic><jats:sub>Ba<\/jats:sub> were not modified by PKA\u2010IP.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The membrane\u2010permeable cyclic nucleotides db\u2010cAMP and 8\u2010Br\u2010cGMP caused little shift of the voltage dependence of the steady\u2010state inactivation and reactivation curves.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Neither of the membrane\u2010permeable cyclic nucleotides db\u2010cAMP or 8\u2010Br\u2010cGMP had additive inhibitory effects on <jats:italic>I<\/jats:italic><jats:sub>Ba<\/jats:sub>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results indicate that two distinct cyclic nucleotide\u2010dependent pathways are present in the guinea\u2010pig gastric antrum, and that both inhibited <jats:italic>I<\/jats:italic><jats:sub>Ba<\/jats:sub> in an independent manner.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2005) <jats:bold>146<\/jats:bold>, 129\u2013138. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0706295\">10.1038\/sj.bjp.0706295<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0706295","type":"journal-article","created":{"date-parts":[[2005,6,20]],"date-time":"2005-06-20T13:09:21Z","timestamp":1119272961000},"page":"129-138","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Modulation of voltage\u2010dependent Ba<sup>2+<\/sup> currents in the guinea\u2010pig gastric antrum by cyclic nucleotide\u2010dependent pathways"],"prefix":"10.1111","volume":"146","author":[{"given":"Hai\u2010Lei","family":"Zhu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G David S","family":"Hirst","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yushi","family":"Ito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriyoshi","family":"Teramoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.48.33851"},{"key":"e_1_2_7_3_1","first-page":"49","article-title":"Catecholamine action on smooth muscle","volume":"39","author":"B\u00dcLBRING E.","year":"1987","journal-title":"Pharmacol. 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