{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T20:22:40Z","timestamp":1790022160919,"version":"4.0.1"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":1065,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:p><jats:list list-type=\"explicit-label\"><jats:list-item><jats:p>The present study attempted to pharmacologically characterize the muscarinic receptor subtypes mediating contraction of human umbilical vein (HUV).<\/jats:p><\/jats:list-item><jats:list-item><jats:p>HUV rings were mounted in organ baths and concentration\u2013response curves were constructed for acetylcholine (ACh) (pEC<jats:sub>50<\/jats:sub>: 6.16\u00b10.04; maximum response 80.00\u00b11.98% of the responses induced by serotonin 10\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>). The absence of endothelium did not modify the contractile responses of ACh in this tissue.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>The role of cholinesterases was evaluated: neither neostigmine (acetylcholinesterase inhibitor) nor iso\u2010OMPA (butyrylcholinesterase inhibitor) modified ACh responses. When both enzymes were simultaneously inhibited, a significantly but little potentiation was observed (control: pEC<jats:sub>50<\/jats:sub>6.33\u00b10.03; double inhibition: pEC<jats:sub>50<\/jats:sub>6.57\u00b10.05).<\/jats:p><\/jats:list-item><jats:list-item><jats:p>Atropine, nonselective muscarinic receptors antagonist, inhibited ACh\u2010induced contraction (p<jats:italic>K<\/jats:italic><jats:sub>B<\/jats:sub>9.67). The muscarinic receptors antagonists pirenzepine (M<jats:sub>1<\/jats:sub>), methoctramine (M<jats:sub>2<\/jats:sub>) and pFHHSiD (M<jats:sub>3<\/jats:sub>) also antagonized responses to ACh. The affinity values estimated for these antagonists against responses evoked by ACh were 7.58, 6.78 and 7.94, respectively. On the other hand, PD 102807 (M<jats:sub>4<\/jats:sub>selective muscarinic receptors antagonist) was ineffective against ACh\u2010induced contraction.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>In presence of a blocking concentration of pirenzepine, pFHHSiFD produced an additional antagonism activity on ACh\u2010induced responses.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>The M<jats:sub>1<\/jats:sub>muscarinic receptors agonist McN\u2010A\u2010343 produced similar maximum but less potent responses than ACh in HUV. The calculated p<jats:italic>A<\/jats:italic><jats:sub>2<\/jats:sub>for pirenzepine against McN\u2010A\u2010343 induced responses was 8.54.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>In conclusion, the data obtained in this study demonstrate the role of M<jats:sub>1<\/jats:sub>muscarinic receptor subtypes and suggest the involvement of M<jats:sub>3<\/jats:sub>muscarinic receptor subtypes in ACh\u2010induced vasoconstriction in HUV rings. In addition, the vasomotor activity evoked by ACh does not seem to be modulated by endothelial factors, and their enzymatic degradation appears to have little functional relevance in this tissue.<\/jats:p><\/jats:list-item><\/jats:list><\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic>(2006)<jats:bold>147<\/jats:bold>, 516\u2013523. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0706654\">10.1038\/sj.bjp.0706654<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0706654","type":"journal-article","created":{"date-parts":[[2006,1,30]],"date-time":"2006-01-30T12:05:03Z","timestamp":1138622703000},"page":"516-523","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Pharmacological characterization of muscarinic receptor subtypes mediating vasoconstriction of human umbilical vein"],"prefix":"10.1111","volume":"147","author":[{"given":"Virginia Andrea","family":"Pujol Lereis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Javier","family":"Hita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro Dar\u00edo","family":"Gobbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela Gomez","family":"Verdi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Cecilia","family":"Rodriguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo Pedro","family":"Rothlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1972.sp009801"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1959.tb00928.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm011116o"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703891"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/S0031-6997(24)01362-0","article-title":"International Union of Pharmacology. XVII. Classification of muscarinic acetylcholine receptors","volume":"50","author":"CAULFIELD M.P.","year":"1998","journal-title":"Pharmacol. Rev."},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1991.tb12174.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705375"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0922-4106(91)90006-4"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1978.235.3.C97"},{"key":"e_1_2_7_11_1","first-page":"608","article-title":"Characterization of vascular muscarinic receptors: rabbit ear artery and bovine coronary artery","volume":"253","author":"DUCKLES S.P.","year":"1990","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_12_1","first-page":"531","article-title":"Muscarinic receptor subtypes and smooth muscle function","volume":"48","author":"EGLEN R.M.","year":"1996","journal-title":"Pharmacol. Rev."},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702320"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4004(05)80443-6"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)67039-3"},{"key":"e_1_2_7_16_1","first-page":"842","article-title":"Pharmacological characterization of the vascular muscarinic receptors mediating relaxation and contraction in rabbit aorta","volume":"258","author":"JAISWAL N.","year":"1991","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(94)90237-2"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(03)00069-9"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.85.24"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0700983"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706147"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2003.10.008"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3109\/10799899209066026"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1159\/000073665"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.55.4.4"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb13486.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15688.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(99)00488-9"},{"key":"e_1_2_7_29_1","first-page":"64","article-title":"Subtypes of muscarinic receptors on adrenergic nerves and vascular smooth muscle of the canine saphenous vein","volume":"241","author":"O'ROURKE S.T.","year":"1987","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1159\/000056169"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1159\/000056131"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(97)00017-8"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091880302"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-002-0637-8"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-002-0636-9"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(96)00927-2"},{"key":"e_1_2_7_37_1","first-page":"665","article-title":"M3 and M1 receptors in cerebral arterioles in vivo: evidence for down\u2010regulated or ineffective M1 when endothelium is intact","volume":"264","author":"SHIMIZU T.","year":"1993","journal-title":"Am. J. Physiol."},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(15)30251-9"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.60.4.517"},{"key":"e_1_2_7_40_1","first-page":"223","article-title":"The muscarinic receptor subtypes in human blood vessels","volume":"56","author":"WALCH L.","year":"2001","journal-title":"Therapie"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703301"},{"key":"e_1_2_7_42_1","first-page":"1293","article-title":"Pharmacological characterization of muscarinic receptor subtype mediating contraction of human peripheral airways","volume":"274","author":"WATSON N.","year":"1995","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(97)00085-5"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02125097"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0706654","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0706654","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0706654","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T14:55:41Z","timestamp":1736261741000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0706654"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,3]]},"references-count":43,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2006,3]]}},"alternative-id":["10.1038\/sj.bjp.0706654"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0706654","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,3]]},"assertion":[{"value":"2009-01-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}