{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:42:51Z","timestamp":1790844171787,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":943,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The aim of this study was to assess the effects of treatment with isoproterenol (ISO, 0.3\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>\u2003day<jats:sup>\u22121<\/jats:sup>, s.c.) for 7 days on the vascular reactivity of rat\u2010isolated aortic rings. Additionally, potential mechanisms underlying the changes that involved the endothelial modulation of contractility were investigated.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Treatment with ISO induced cardiac hypertrophy without changes in haemodynamic parameters. Aortic rings from ISO\u2010treated rats showed an increase in the contraction response to phenylephrine (PHE) and serotonin, but did not change relaxations produced by acetylcholine or isoproterenol. Removal of the endothelium increased the responses to PHE in both groups. However, this procedure was less effective in ISO\u2010treated as compared with control rats. Endothelial cell removal abolished the increase in the response to PHE in ISO\u2010treated rats. The presence of <jats:italic>N<\/jats:italic><jats:sup><jats:italic>\u03c9<\/jats:italic><\/jats:sup>\u2010nitro\u2010<jats:sc>L<\/jats:sc>\u2010arginine methyl ester shifted the concentration\u2013response curve to PHE to the left in both groups of rats. However, this effect was more pronounced in the ISO group. In addition, aminoguanidine (50\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) potentiated the actions of PHE only in the ISO group. ISO treatment increased nitric oxide synthase (NOS) activity and neuronal NOS and endothelial NOS protein expression in the aorta.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Neither losartan (10\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) nor indomethacin (10\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) abolished the effects of ISO on the actions of PHE. Superoxide dismutase (SOD, 150\u2003U\u2003ml<jats:sup>\u22121<\/jats:sup>) and <jats:sc>L<\/jats:sc>\u2010arginine (5\u2003m<jats:sc>M<\/jats:sc>), but neither catalase (300\u2003U\u2003ml<jats:sup>\u22121<\/jats:sup>) nor apocynin (100\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>), blocked the effect of ISO treatment. In addition, we observed an increase in superoxide anion levels as measured by ethidium bromide fluorescence and of copper and zinc superoxide dismutase protein expression in ISO\u2010treated rats.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In conclusion, our data suggest that ISO treatment alters the endothelial cell\u2010mediated modulation of the contraction to PHE in rat aorta. The increased maximal response of PHE seems to be due to an increase in superoxide anion generation, which inactivates some of the basal NO produced and counteracts NO\u2010mediated negative modulation even in the presence of high NO production and antioxidant defence.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2006) <jats:bold>148<\/jats:bold>, 629\u2013639. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0706749\">10.1038\/sj.bjp.0706749<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0706749","type":"journal-article","created":{"date-parts":[[2006,5,16]],"date-time":"2006-05-16T09:52:50Z","timestamp":1147773170000},"page":"629-639","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Changes in vascular reactivity following administration of isoproterenol for 1 week: a role for endothelial modulation"],"prefix":"10.1111","volume":"148","author":[{"given":"Ana Paula C","family":"Davel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa Mitiko","family":"Kawamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristoforo","family":"Scavone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalton V","family":"Vassallo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana V","family":"Rossoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000056601.29613.DD"},{"key":"e_1_2_7_3_1","first-page":"149","article-title":"Influence of ISO\u2010induced cardiac hypertrophy on oxidative myocardial stress","volume":"32","author":"BALTA N.","year":"1995","journal-title":"Rom. 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