{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:24:07Z","timestamp":1790972647751,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":973,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:p><jats:list list-type=\"explicit-label\"><jats:list-item><jats:p>Although previous reports have suggested that the sigma 1 (<jats:italic>\u03c3<\/jats:italic><jats:sub>1<\/jats:sub>) receptor may be involved in pain sensation, its specific site of action has not been elucidated. The aim of present study was to determine the role of the spinal<jats:italic>\u03c3<\/jats:italic><jats:sub>1<\/jats:sub>receptor in formalin\u2010induced pain behavior, spinal cord Fos expression and phosphorylation of<jats:italic>N<\/jats:italic>\u2010methyl\u2010<jats:sc>D<\/jats:sc>\u2010aspartate receptor subunit 1 (pNR1).<\/jats:p><\/jats:list-item><jats:list-item><jats:p>Intrathecal (i.t.) pretreatment with the selective<jats:italic>\u03c3<\/jats:italic><jats:sub>1<\/jats:sub>receptor antagonist, BD\u20101047 (<jats:italic>N<\/jats:italic>\u2010[2\u2010(3,4\u2010dichlorophenyl)ethyl]\u2010<jats:italic>N<\/jats:italic>\u2010methyl\u20102\u2010(dimethylamino)ethylamine dihydrobromide) (10\u2013100\u2003nmol) dose dependently reduced formalin\u2010induced pain behaviors in second phase, but not first phase, of the formalin test. I.t. injection of BD\u20101047 also reduced formalin\u2010evoked Fos expression and pNR1 at the protein kinase C\u2010dependent site, serine\u2010896 (Ser896) and the protein kinase A\u2010dependent site, serine\u2010897 (Ser897) in spinal dorsal horn.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>i.t. BMY\u201014802 ((<jats:italic>\u03b1<\/jats:italic>\u2010(4\u2010fluorophenyl)\u20104\u2010(5\u2010fluoro\u20102\u2010pyrimidinyl)\u20101\u2010piperazinebutanol hydrochloride) (10\u2013100\u2003nmol,<jats:italic>\u03c3<\/jats:italic><jats:sub>1<\/jats:sub>receptor antagonist and 5\u2010HT<jats:sub>1A<\/jats:sub>receptor agonist) dose dependently reduced formalin\u2010induced pain behaviors in both phases. However, the 5\u2010HT<jats:sub>1A<\/jats:sub>receptor might not be involved in the antinociceptive effect of BMY\u201014802 on the second phase, since i.t. pretreatment with the 5\u2010HT<jats:sub>1A<\/jats:sub>receptor antagonist propranolol ((<jats:italic>S<\/jats:italic>)\u20101\u2010isopropylamino\u20103\u2010(1\u2010naphthyloxy)\u20102\u2010propanol hydrochloride) (injected 10\u2003min prior to i.t. BMY\u201014802) partially blocked the effect of BMY\u201014802 on the first phase of the formalin test but did not affect the inhibitory effect of BMY\u201014802 on the second phase. In addition, i.t. BMY\u201014802 significantly reduced formalin\u2010evoked Fos expression and pNR1 (Ser896 and Ser897) expression in spinal dorsal horn.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>The results of this study suggest that selective blockage of spinal<jats:italic>\u03c3<\/jats:italic><jats:sub>1<\/jats:sub>receptors can reduce pain behaviors, spinal cord Fos expression and pNR1 (Ser896 and Ser897) expression associated with the second phase of the formalin test.<\/jats:p><\/jats:list-item><\/jats:list><\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic>(2006)<jats:bold>148<\/jats:bold>, 490\u2013498. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0706764\">10.1038\/sj.bjp.0706764<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0706764","type":"journal-article","created":{"date-parts":[[2006,5,8]],"date-time":"2006-05-08T13:33:10Z","timestamp":1147095190000},"page":"490-498","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":90,"title":["Intrathecal treatment with<i>\u03c3<\/i><sub>1<\/sub>receptor antagonists reduces formalin\u2010induced phosphorylation of NMDA receptor subunit 1 and the second phase of formalin test in mice"],"prefix":"10.1111","volume":"148","author":[{"given":"Hyun\u2010Woo","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young\u2010Bae","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dae\u2010Hyun","family":"Roh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seo\u2010Yeon","family":"Yoon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho\u2010Jae","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kee\u2010Won","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvin J","family":"Beitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jang\u2010Hern","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(94)90106-6"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(96)03285-X"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(00)00014-2"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1461145701002760"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2004.03506.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2005.03.016"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8069-1-25"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-005-0127-z"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2005.01.036"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1016\/S0022-3565(24)36427-4","article-title":"Efficacy of spinal NMDA receptor antagonism in formalin hyperalgesia and nerve injury evoked allodynia in the rat","volume":"280","author":"CHAPLAN S.R.","year":"1997","journal-title":"J. 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