{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T20:13:59Z","timestamp":1771013639257,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":943,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Envenomation by the snake <jats:italic>Bothrops jararaca<\/jats:italic> is typically associated with hemostatic abnormalities including pro\u2010 and anticoagulant disturbances. Glycyrrhizin (GL) is a plant\u2010derived thrombin inhibitor that also exhibits <jats:italic>in vivo<\/jats:italic> antithrombotic properties. Here, we evaluated the ability of GL to counteract the hemostatic abnormalities promoted by <jats:italic>B. jararaca<\/jats:italic> venom.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>GL inhibited the human fibrinogen clotting (IC<jats:sub>50<\/jats:sub>=\u223c1.0\u2003mg\u2003ml<jats:sup>\u22121<\/jats:sup>; 1.2\u2003m<jats:sc>M<\/jats:sc>), <jats:italic>H<\/jats:italic>\u2010<jats:sc>D<\/jats:sc>\u2010phenylalanyl\u2010<jats:sc>L<\/jats:sc>\u2010pipecolyl\u2010<jats:sc>L<\/jats:sc>\u2010arginine\u2010<jats:italic>p<\/jats:italic>\u2010nitroanilide dihydrochloride hydrolysis (IC<jats:sub>50<\/jats:sub>=\u223c0.4\u2003mg\u2003ml<jats:sup>\u22121<\/jats:sup>; 0.47\u2003m<jats:sc>M<\/jats:sc>) and platelet aggregation (IC<jats:sub>50<\/jats:sub>=\u223c0.28\u2003mg\u2003ml<jats:sup>\u22121<\/jats:sup>; 0.33\u2003m<jats:sc>M<\/jats:sc>) induced by <jats:italic>B. jararaca<\/jats:italic> venom, <jats:italic>in vitro<\/jats:italic>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The <jats:italic>in vivo<\/jats:italic> effect of GL was tested in rats using a model of venous thrombosis in which intravenous (i.v.) administration of <jats:italic>B. jararaca<\/jats:italic> venom (100\u2003<jats:italic>\u03bc<\/jats:italic>g\u2003kg<jats:sup>\u22121<\/jats:sup>) produced in all animals a thrombus with a mean weight of 10.6\u00b11.7\u2003mg.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Prior administration of GL (180\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>) or antibothropic serum (27\u2003<jats:italic>\u03bc<\/jats:italic>l\u2003kg<jats:sup>\u22121<\/jats:sup>) inhibited thrombus formation by 86 and 67%, respectively. Remarkably, co\u2010administration of ineffective doses of GL and antibothropic serum markedly decreased thrombus weight, suggesting a synergistic effect.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Co\u2010administration of GL with antibothropic serum abolished venom\u2010induced bleeding. <jats:italic>Ex vivo<\/jats:italic> clotting times showed that rat plasma was non\u2010clotting after i.v. administration of <jats:italic>B. jararaca<\/jats:italic> venom. Treatment with GL, antibothropic serum or both before venom administration efficiently prevented this abnormality.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Altogether, we demonstrate here that GL prevents both <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> venom\u2010induced changes in hemostasis, suggesting a potential antiophidic activity.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2006) <jats:bold>148<\/jats:bold>, 807\u2013813. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0706786\">10.1038\/sj.bjp.0706786<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0706786","type":"journal-article","created":{"date-parts":[[2006,6,5]],"date-time":"2006-06-05T11:06:19Z","timestamp":1149505579000},"page":"807-813","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Counteracting effect of glycyrrhizin on the hemostatic abnormalities induced by <i>Bothrops jararaca<\/i> snake venom"],"prefix":"10.1111","volume":"148","author":[{"given":"Mariane","family":"Assafim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos S","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1via S","family":"Frattani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge A","family":"Guimar\u00e3es","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robson Q","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Russolina B","family":"Zingali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/158878a0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"82","DOI":"10.4269\/ajtmh.1993.48.82","article-title":"Snakebite\u2010induced acute renal failure: an experimental model","volume":"48","author":"BURDMANN E.A.","year":"1993","journal-title":"Am. 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