{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:49:18Z","timestamp":1791960558733,"version":"4.3.4"},"reference-count":60,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":943,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>We developed a novel method to isolate nonpigmented epithelial (NPE) cells from porcine eyes in order to examine Na,K\u2010ATPase responses to nitric oxide (NO) donors specifically in the epithelium.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Cells were treated with NO donors and other test compounds for 20\u2003min prior to Na,K\u2010ATPase activity measurement.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>NO donors, sodium nitroprusside (SNP, 1\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>\u20131\u2003m<jats:sc>M<\/jats:sc>), sodium azide (100\u2003n<jats:sc>M<\/jats:sc>\u20131\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) and <jats:italic>S<\/jats:italic>\u2010nitroso\u2010<jats:italic>N<\/jats:italic>\u2010acetylpenicillamine (1\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>\u20131\u2003m<jats:sc>M<\/jats:sc>) caused significant concentration\u2010dependent inhibition of Na, K\u2010ATPase activity. Detection of nitrite in the medium of <jats:sc>L<\/jats:sc>\u2010arginine and SNP\u2010treated NPE confirmed NO generation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Concentration\u2010dependent inhibition of Na,K\u2010ATPase was also obtained by <jats:sc>L<\/jats:sc>\u2010arginine (1\u20133\u2003m<jats:sc>M<\/jats:sc>), a physiological precursor of NO and 8p\u2010CPT\u2010cGMP (1\u2013100\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>), a cell permeable analog of cGMP. The <jats:sc>L<\/jats:sc>\u2010arginine effect was abolished when the NO synthesizing enzyme, NO\u2010synthase, was inhibited by <jats:sc>L<\/jats:sc>\u2010NAME (100\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The inhibitory effect of SNP or sodium azide on Na,K\u2010ATPase activity was suppressed by soluble guanylate cyclase (sGC) inhibitors, ODQ (10\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) or methylene blue (10\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The inhibitory effect of 8p\u2010CPT\u2010cGMP on Na,K\u2010ATPase was abolished by protein kinase G (PKG) inhibitors, H\u20108 (1\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>) and H\u20109 (20\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>M<\/jats:sc>), but not by the protein kinase A (PKA) inhibitor H\u201089 (100\u2003n<jats:sc>M<\/jats:sc>). H\u20108 and H\u20109 partially suppressed the inhibitory effect of SNP on Na,K\u2010ATPase.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Taken together the results indicate that Na,K\u2010ATPase inhibition response to NO donors involves activation of sGC, generation of cGMP and activation of PKG. These findings suggest that Na, K\u2010ATPase inhibition in NPE may contribute to the ability of NO donors to reduce aqueous humor secretion.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2006) <jats:bold>148<\/jats:bold>, 871\u2013880. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0706795\">10.1038\/sj.bjp.0706795<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0706795","type":"journal-article","created":{"date-parts":[[2006,6,12]],"date-time":"2006-06-12T13:27:17Z","timestamp":1150118837000},"page":"871-880","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["NO donors inhibit Na,K\u2010ATPase activity by a protein kinase G\u2010dependent mechanism in the nonpigmented ciliary epithelium of the porcine eye"],"prefix":"10.1111","volume":"148","author":[{"given":"Mohammad","family":"Shahidullah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas A","family":"Delamere","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"1711","article-title":"Decreased intraocular pressure induced by nitric oxide donors is correlated to nitrite production in the rabbit eye","volume":"37","author":"BEHAR\u2010COHEN F.F.","year":"1996","journal-title":"Invest. Ophthalmol. Visual Sci."},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1076\/0271-3683(200006)2061-YFT511","article-title":"The effect of L\u2010arginine on intraocular pressure in the human eye","volume":"20","author":"CHUMAN H.","year":"2000","journal-title":"Curr. Eye Res."},{"key":"e_1_2_7_4_1","volume-title":"The Eye's Aqueous Humor: from Secretion to Glaucoma","author":"CIVAN M.M.","year":"1998"},{"key":"e_1_2_7_5_1","first-page":"1","volume-title":"The Eye's Aqueous Humor: from Secretion to Glaucoma","author":"CIVAN M.M.","year":"1998"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240000302"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(98)00928-5"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/24884"},{"key":"e_1_2_7_9_1","first-page":"430","article-title":"The influence of cyclic AMP upon Na,K\u2010ATPase activity in rabbit ciliary epithelium","volume":"33","author":"DELAMERE N.A.","year":"1992","journal-title":"Invest. Ophthalmol. Visual Sci."},{"key":"e_1_2_7_10_1","first-page":"866","article-title":"Indomethacin alters the Na,K\u2010ATPase response to protein kinase C activation in cultured rabbit nonpigmented ciliary epithelium","volume":"38","author":"DELAMERE N.A.","year":"1997","journal-title":"Invest. Ophthalmol. Visual Sci."},{"key":"e_1_2_7_11_1","first-page":"2625","article-title":"Carbachol and nitric oxide inhibition of Na,K\u2010ATPase activity in bovine ciliary processes","volume":"42","author":"ELLIS D.Z.","year":"2001","journal-title":"Invest. Ophthalmol. Visual Sci."},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.279.6.C1685"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(90)90049-Z"},{"key":"e_1_2_7_14_1","doi-asserted-by":"crossref","first-page":"2935","DOI":"10.1016\/S0021-9258(19)39891-6","article-title":"Cellular distribution and differential gene expression of the three alpha subunit isoforms of the Na,K\u2010ATPase in the ocular ciliary epithelium","volume":"265","author":"GHOSH S.","year":"1990","journal-title":"J. Biol. Chem."},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117895"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006324-200102000-00008"},{"key":"e_1_2_7_17_1","first-page":"639","article-title":"Protective effect of nitric oxide on ischemic retina","volume":"101","author":"IMAI N.","year":"1997","journal-title":"Nippon Ganka Gakkai Zasshi \u2013 Acta Societatis Ophthalmol. Japonicae"},{"key":"e_1_2_7_18_1","first-page":"577","article-title":"Naphthalenesulfonamides as calmodulin antagonists and protein kinase inhibitors","volume":"29","author":"INAGAKI M.","year":"1986","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_7_19_1","doi-asserted-by":"crossref","first-page":"2922","DOI":"10.1016\/S0021-9258(18)89453-4","article-title":"N\u2010(2\u2010Aminoethyl)\u20105\u2010isoquinolinesulfonamide, a newly synthesized protein kinase inhibitor, functions as a ligand in affinity chromatography. Purification of Ca2+\u2010activated, phospholipid\u2010dependent and other protein kinases","volume":"260","author":"INAGAKI M.","year":"1985","journal-title":"J. Biol. Chem."},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.3.C703"},{"key":"e_1_2_7_21_1","first-page":"2769","article-title":"Effect of 8\u2010Br cGMP on aqueous humor dynamics in monkeys","volume":"35","author":"KEE C.","year":"1994","journal-title":"Invest. Ophthalmol. Visual Sci."},{"key":"e_1_2_7_22_1","first-page":"311","article-title":"Modulation of retinal dopaminergic cells by nitric oxide. A protective effect on NMDA\u2010induced retinal injury","volume":"18","author":"KITAOKA Y.","year":"2004","journal-title":"In Vivo"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2980249"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v11.i23.3554"},{"key":"e_1_2_7_25_1","first-page":"2385","article-title":"Atrial natriuretic peptides. Effects on intraocular pressure, cGMP, and aqueous flow","volume":"30","author":"KORENFELD M.S.","year":"1989","journal-title":"Invest. Ophthalmol. Visual Sci."},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.26.2.119.14511"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(84)90096-4"},{"key":"e_1_2_7_28_1","first-page":"F673","article-title":"Protein phosphatase\u20101 in the kidney: evidence for a role in the regulation of medullary Na(+)\u2010K(+)\u2010ATPase","volume":"269","author":"LI D.","year":"1995","journal-title":"Am. J. Physiol."},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1999.277.6.F859"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2002-30643"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1159\/000026342"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.18.5.375.5355"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1089\/jop.1997.13.1"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1089\/108076801750295263"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2002.tb04083.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.922"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.24.2.114.8162"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6257(01)00198-9"},{"key":"e_1_2_7_39_1","first-page":"956","article-title":"Nitrovasodilators as a new class of ocular hypotensive agents","volume":"260","author":"NATHANSON J.A.","year":"1992","journal-title":"J. Pharmacol. Exp. Therapeut."},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00334.2001"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(86)90046-1"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(95)01165-X"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.68031312.x"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1159\/000159039"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-005-5616-1"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702681"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.02-0397"},{"key":"e_1_2_7_48_1","first-page":"1","article-title":"cGMP, sodiun nitroprusside and sodium azide reduce aqueous humour formation in the isolated arterially perfused pig eye","volume":"144","author":"SHAHIDULLAH M.","year":"2005","journal-title":"Br. J. Pharmacol."},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.12-08-03071.1992"},{"key":"e_1_2_7_50_1","first-page":"871","article-title":"Na+,K+\u2010ATPase activity is inhibited in cultured intestinal epithelial cells by endotoxin or nitric oxide","volume":"15","author":"SUZUKI Y.","year":"2005","journal-title":"Int. J. Mol. Med."},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(99)00021-6"},{"key":"e_1_2_7_52_1","first-page":"2671","article-title":"Ocular hypotensive mechanism of intravitreally injected brain natriuretic peptide in rabbit","volume":"37","author":"TAKASHIMA Y.","year":"1996","journal-title":"Invest. Ophthalmol. Visual Sci."},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.00284"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1444-0938.2002.tb02384.x"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.8.3462"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00427.2003"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(88)56022-6"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(05)80116-2"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010912929042"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(89)90470-3"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1089-8603(02)00003-4"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0706795","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.bjp.0706795","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.bjp.0706795","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T08:14:11Z","timestamp":1697098451000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/sj.bjp.0706795"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,7]]},"references-count":60,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,7]]}},"alternative-id":["10.1038\/sj.bjp.0706795"],"URL":"https:\/\/doi.org\/10.1038\/sj.bjp.0706795","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,7]]},"assertion":[{"value":"2009-01-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}