{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T07:04:49Z","timestamp":1777964689343,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":912,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:p>\n                    <jats:list list-type=\"explicit-label\">\n                      <jats:list-item>\n                        <jats:p>\n                          Inhibition of the Na\n                          <jats:sup>+<\/jats:sup>\n                          \u2013K\n                          <jats:sup>+<\/jats:sup>\n                          \u20132Cl\n                          <jats:sup>\u2212<\/jats:sup>\n                          cotransporter (NKCC1) with bumetanide reduced contractile responses to phenylephrine (PE) in male rat aortas (129\u00b14% of 60\u2003m\n                          <jats:sc>M<\/jats:sc>\n                          KCl\u2010induced contraction control vs 108\u00b17% bumetanide; PE 10\n                          <jats:sup>\u22125<\/jats:sup>\n                          \u2003\n                          <jats:sc>M<\/jats:sc>\n                          ;\n                          <jats:italic>P<\/jats:italic>\n                          &lt;0.01) but did not change equivalent responses in female rat aortas. Removal of the endothelium blunted the effect of NKCC1 inhibition on the response to PE (10\n                          <jats:sup>\u22125<\/jats:sup>\n                          \u2003\n                          <jats:sc>M<\/jats:sc>\n                          ) in males, whereas in denuded aorta from female rats, bumetanide reduced this response (162\u00b15% control vs 146\u00b13% bumetanide;\n                          <jats:italic>P<\/jats:italic>\n                          &lt;0.05).\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          NKCC1 basal activity did not show gender differences in intact aortic rings, but in the presence of PE, bumetanide\u2010sensitive\n                          <jats:sup>86<\/jats:sup>\n                          Rb\n                          <jats:sup>+<\/jats:sup>\n                          \/K\n                          <jats:sup>+<\/jats:sup>\n                          uptake increased more in male than female aortas (179\u00b18 in males vs 158\u00b15\u2003nmol\n                          <jats:sup>86<\/jats:sup>\n                          Rb\n                          <jats:sup>+<\/jats:sup>\n                          \/K\n                          <jats:sup>+<\/jats:sup>\n                          \u2003min\n                          <jats:sup>\u22121<\/jats:sup>\n                          \u2003(g aorta)\n                          <jats:sup>\u22121<\/jats:sup>\n                          in females;\n                          <jats:italic>P<\/jats:italic>\n                          &lt;0.05). PE did not stimulate NKCC1 activity in denuded aorta from male rats. However, in female rats, PE increased NKCC1 activity similarly in both denuded (169\u00b111\u2003nmol\n                          <jats:sup>86<\/jats:sup>\n                          Rb\n                          <jats:sup>+<\/jats:sup>\n                          \/K\n                          <jats:sup>+<\/jats:sup>\n                          \u2003min\n                          <jats:sup>\u22121<\/jats:sup>\n                          \u2003(g aorta)\n                          <jats:sup>\u22121<\/jats:sup>\n                          ) and intact aortas.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Ovariectomy increased the bumetanide\u2010sensitive\n                          <jats:sup>86<\/jats:sup>\n                          Rb\n                          <jats:sup>+<\/jats:sup>\n                          \/K\n                          <jats:sup>+<\/jats:sup>\n                          uptake increase elicited by PE (223\u00b117\u2003nmol\n                          <jats:sup>86<\/jats:sup>\n                          Rb\n                          <jats:sup>+<\/jats:sup>\n                          \/K\n                          <jats:sup>+<\/jats:sup>\n                          \u2003min\n                          <jats:sup>\u22121<\/jats:sup>\n                          \u2003(g aorta)\n                          <jats:sup>\u22121<\/jats:sup>\n                          ) and hormone replacement with 17\n                          <jats:italic>\u03b2<\/jats:italic>\n                          \u2010estradiol prevented this effect (159\u00b129\u2003nmol\n                          <jats:sup>86<\/jats:sup>\n                          Rb\n                          <jats:sup>+<\/jats:sup>\n                          \/K\n                          <jats:sup>+<\/jats:sup>\n                          \u2003min\n                          <jats:sup>\u22121<\/jats:sup>\n                          \u2003(g aorta)\n                          <jats:sup>\u22121<\/jats:sup>\n                          ).\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Na\n                          <jats:sup>+<\/jats:sup>\n                          ,K\n                          <jats:sup>+<\/jats:sup>\n                          \u2010ATPase basal activity, measured as ouabain\u2010sensitive\n                          <jats:sup>86<\/jats:sup>\n                          Rb\n                          <jats:sup>+<\/jats:sup>\n                          \/K\n                          <jats:sup>+<\/jats:sup>\n                          uptake, was similar in male and female rats, but the effect of PE was significantly less in intact male aortas (232\u00b116 in males vs 296\u00b125\u2003nmol\n                          <jats:sup>86<\/jats:sup>\n                          Rb\n                          <jats:sup>+<\/jats:sup>\n                          \/K\n                          <jats:sup>+<\/jats:sup>\n                          \u2003min\n                          <jats:sup>\u22121<\/jats:sup>\n                          \u2003(g aorta)\n                          <jats:sup>\u22121<\/jats:sup>\n                          in females;\n                          <jats:italic>P<\/jats:italic>\n                          &lt;0.05).\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Our results suggest that PE induced activation of NKCC1 and Na\n                          <jats:sup>+<\/jats:sup>\n                          ,K\n                          <jats:sup>+<\/jats:sup>\n                          \u2010ATPase in the rat aorta in a gender\u2010dependent way.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>British Journal of Pharmacology<\/jats:italic>\n                    (2006)\n                    <jats:bold>148<\/jats:bold>\n                    , 964\u2013972. doi:\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0706818\">10.1038\/sj.bjp.0706818<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1038\/sj.bjp.0706818","type":"journal-article","created":{"date-parts":[[2006,6,26]],"date-time":"2006-06-26T08:04:38Z","timestamp":1151309078000},"page":"964-972","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Na\n                    <sup>+<\/sup>\n                    \u2013K\n                    <sup>+<\/sup>\n                    \u20132Cl\n                    <sup>\u2212<\/sup>\n                    cotransporter is implicated in gender differences in the response of the rat aorta to 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