{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T06:42:28Z","timestamp":1792392148135,"version":"4.4.0"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":790,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:sec><jats:title>Background and purpose:<\/jats:title><jats:p>The natriuretic peptides, ANP and BNP, modulate vascular smooth muscle tone in human conduit arteries. Surprisingly, the natriuretic peptide receptor\u2010A (NPR\u2010A) has not been visualized using radioligand binding in these vessels. A new member of this peptide family, <jats:italic>Dendroaspis<\/jats:italic> natriuretic peptide (DNP) identified from snake venom, has been proposed to be present in human plasma and endothelial cells. Also, recently a novel radioligand, [<jats:sup>125<\/jats:sup>I]\u2010DNP, has been characterized as selective for NPR\u2010A in human heart.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental approach:<\/jats:title><jats:p>Our aims were to investigate expression and function of NPR\u2010A receptors in human mammary artery using [<jats:sup>125<\/jats:sup>I]\u2010DNP to quantify receptor density, immunocytochemistry to delineate the cellular distribution of the receptor and <jats:italic>in vitro<\/jats:italic> pharmacology to compare DNP induced vasodilatation to that of ANP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key results:<\/jats:title><jats:p>Saturable, sub\u2010nanomolar affinity [<jats:sup>125<\/jats:sup>I]\u2010DNP binding was detected to smooth muscle of mammary artery, with receptor density of \u223c2\u2003fmol\u2003mg<jats:sup>\u20131<\/jats:sup> protein, comparable to that of other vasoactive peptides. NPR\u2010A immunoreactivity was localised to vascular smooth muscle cells and this was confirmed with fluorescence dual labelling. NPR\u2010A expression was not detected in the endothelium. Like ANP, DNP fully reversed the constrictor response to ET\u20101 in endothelium intact or denuded mammary artery, with comparable nanomolar potencies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications:<\/jats:title><jats:p>This is the first characterization of NPR\u2010A in human mammary artery using [<jats:sup>125<\/jats:sup>I]\u2010DNP and we provide evidence for the presence of receptor protein on vascular smooth muscle cells, but not endothelial cells. This implies that the observed vasodilatation is predominantly mediated via direct activation of smooth muscle NPR\u2010A.<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2006) <jats:bold>149<\/jats:bold>, 838\u2013844. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0706924\">10.1038\/sj.bjp.0706924<\/jats:ext-link><\/jats:p><\/jats:sec>","DOI":"10.1038\/sj.bjp.0706924","type":"journal-article","created":{"date-parts":[[2006,10,17]],"date-time":"2006-10-17T12:22:29Z","timestamp":1161087749000},"page":"838-844","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Characterization of the snake venom ligand [<sup>125<\/sup>I]\u2010DNP binding to natriuretic peptide receptor\u2010A in human artery and potent DNP mediated vasodilatation"],"prefix":"10.1111","volume":"149","author":[{"given":"G","family":"Singh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J J","family":"Maguire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R E","family":"Kuc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J N","family":"Skepper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Fidock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A 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