{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T02:57:48Z","timestamp":1784429868965,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":790,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:sec><jats:title>Background and purpose.<\/jats:title><jats:p>The aim of this study was to investigate, in vascular smooth muscle cells, the mechanical and electrophysiological effects of (+\/\u2212)\u2010naringenin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental approach.<\/jats:title><jats:p>Aorta ring preparations and single tail artery myocytes were employed for functional and patch\u2010clamp experiments, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key results.<\/jats:title><jats:p>(+\/\u2212)\u2010Naringenin induced concentration\u2010dependent relaxation in endothelium\u2010denuded rat aortic rings pre\u2010contracted with either 20 mM KCl or noradrenaline (pIC<jats:sub>50<\/jats:sub> values of 4.74 and 4.68, respectively). Tetraethylammonium, iberiotoxin, 4\u2010aminopyridine and 60 mM KCl antagonised (+\/\u2212)\u2010naringenin\u2010induced vasorelaxation, while glibenclamide did not produce any significant antagonism. Naringin [(+\/\u2212)\u2010naringenin 7\u2010\u03b2\u2010neohesperidoside] caused a concentration\u2010dependent relaxation of rings pre\u2010contracted with 20 mM KCl, although its potency and efficacy were significantly lower than those of (+\/\u2212)\u2010naringenin. In rat tail artery myocytes, (+\/\u2212)\u2010naringenin increased large conductance Ca<jats:sup>2+<\/jats:sup>\u2010activated K<jats:sup>+<\/jats:sup> (BK<jats:sub>Ca<\/jats:sub>) currents in a concentration\u2010dependent manner; this stimulation was iberiotoxin\u2010sensitive and fully reversible upon drug wash\u2010out. (+\/\u2212)\u2010Naringenin accelerated the activation kinetics of BK<jats:sub>Ca<\/jats:sub> current, shifted, by 22 mV, the voltage dependence of the activation curve to more negative potentials, and decreased the slope of activation. (+\/\u2212)\u2010Naringenin\u2010induced stimulation of BK<jats:sub>Ca<\/jats:sub> current was insensitive either to changes in the intracellular Ca<jats:sup>2+<\/jats:sup> concentration or to the presence, in the pipette solution, of the fast Ca<jats:sup>2+<\/jats:sup> chelator BAPTA. However, such stimulation was diminished when the K<jats:sup>+<\/jats:sup> gradient across the membrane was reduced.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications.<\/jats:title><jats:p>The vasorelaxant effect of the naturally\u2010occurring flavonoid (+\/\u2212)\u2010naringenin on endothelium\u2010denuded vessels was due to the activation of BK<jats:sub>Ca<\/jats:sub> channels in myocytes.<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2006) <jats:bold>149<\/jats:bold>, 1013\u20131021. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0706951\">10.1038\/sj.bjp.0706951<\/jats:ext-link><\/jats:p><\/jats:sec>","DOI":"10.1038\/sj.bjp.0706951","type":"journal-article","created":{"date-parts":[[2006,11,6]],"date-time":"2006-11-06T15:30:26Z","timestamp":1162827026000},"page":"1013-1021","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":112,"title":["(+\/\u2212)\u2010Naringenin as large conductance Ca<sup>2+<\/sup>\u2010activated K<sup>+<\/sup> (BK<sub>Ca<\/sub>) channel opener in vascular smooth muscle 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