{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T22:40:24Z","timestamp":1772923224371,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":759,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:sec><jats:title>Background and purpose:<\/jats:title><jats:p>Kinins have an important role in inflammatory cystitis and in animal pathophysiological models, by acting on epithelium, fibroblasts, sensory innervation and smooth muscle. The aim of this study was to characterize the receptors responsible for direct motor responses induced by kinins on human detrusor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental approach:<\/jats:title><jats:p>Human detrusor cells from biopsies were isolated and mantained in culture. B<jats:sub>1<\/jats:sub>and B<jats:sub>2<\/jats:sub>kinin receptors were characterized by means of radioligand and functional experiments (PI accumulation and PGE<jats:sub>2<\/jats:sub>release).<\/jats:p><\/jats:sec><jats:sec><jats:title>Key results:<\/jats:title><jats:p>[<jats:sup>3<\/jats:sup>H]\u2010[desArg<jats:sup>9<\/jats:sup>]\u2010Lys\u2010BK and [<jats:sup>3<\/jats:sup>H]\u2010BK saturation studies indicated receptor density (B<jats:sub>max<\/jats:sub>) and<jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub>values of 19 or 113\u2003fmol\u2003mg<jats:sup>\u22121<\/jats:sup>, and 0.16 or 0.11 nM for the B<jats:sub>1<\/jats:sub>or B<jats:sub>2<\/jats:sub>receptors, respectively. Inhibition binding studies indicated the selectivity of the B<jats:sub>1<\/jats:sub>receptor antagonist [desArg<jats:sup>9<\/jats:sup>Leu<jats:sup>8<\/jats:sup>]\u2010Lys\u2010BK and of the B<jats:sub>2<\/jats:sub>receptor antagonists Icatibant and MEN16132. [DesArg<jats:sup>9<\/jats:sup>]\u2010Lys\u2010BK and BK induced PI accumulation with an EC<jats:sub>50<\/jats:sub>of 1.6 and 1.4 nM and different maximal responses (E<jats:sub>max<\/jats:sub>of [desArg<jats:sup>9<\/jats:sup>]\u2010Lys\u2010BK was 10% of BK). BK also induced prostaglandin E<jats:sub>2<\/jats:sub>release (EC<jats:sub>50<\/jats:sub>2.3 nM), whereas no response was detected with the B<jats:sub>1<\/jats:sub>receptor agonist. The incubation of detrusor smooth muscle cells with interleukin 1\u03b2 (IL\u20101\u03b2) or tumour necrosis factor\u2010<jats:italic>\u03b1<\/jats:italic>(TNF\u2010<jats:italic>\u03b1<\/jats:italic>) (10 ng ml<jats:sup>\u22121<\/jats:sup>) induced a time\u2010dependent increase in radioligand\u2010specific binding, which was greater for the B<jats:sub>1<\/jats:sub>than for the B<jats:sub>2<\/jats:sub>receptor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications:<\/jats:title><jats:p>Human detrusor smooth muscle cells in culture retain kinin receptors, and represent a suitable model to investigate the mechanisms and changes that occur under chronic inflammatory conditions.<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic>(2007)<jats:bold>150<\/jats:bold>, 192\u2013199. doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" 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