{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T07:24:32Z","timestamp":1777793072561,"version":"3.51.4"},"reference-count":34,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:p>\n                    The short- and long-term neuroprotective effects of 2-iminobiotin, a selective inhibitor of neuronal and inducible nitric oxide synthase, were studied in 12-day-old rats following hypoxia-ischemia. Hypoxia-ischemia was induced by occlusion of the right carotid artery followed by 90 minutes of hypoxia (FiO\n                    <jats:sub>2<\/jats:sub>\n                    0.08). Immediately on reoxygenation, 12 and 24 hours later the rats were treated with vehicle or 2-iminobiotin at a dose of 5.5, 10, 30, or 60 mg\/kg per day. Histologic analysis of brain damage was performed at 6 weeks after hypoxia-ischemia. To assess early changes of cerebral tissue, levels of HSP70, nitrotyrosine, and cytochrome c were determined 24 hours after reoxygenation. Significant neuroprotection was obtained using a dose of 30 mg\/kg per day of 2-iminobiotin. Levels of HSP70 were increased in the ipsilateral hemisphere in both groups ( P&lt;0.05), but the increase was significantly ( P&lt;0.05) less in the rats receiving the optimal dose of 2-iminobiotin (30 mg\/kg per day). Hypoxia-ischemia did not lead to increased levels of nitrotyrosine, nor did 2-iminobiotin influence levels of nitrotyrosine. In contrast, hypoxia-ischemia induced an increase in cytochrome c level that was prevented by 2-iminobiotin. In conclusion, 2-iminobiotin administered after hypoxia-ischemia provides long-term neuroprotection. This neuroprotection is obtained by mechanisms other than a reduction of nitrotyrosine formation in proteins.\n                  <\/jats:p>","DOI":"10.1038\/sj.jcbfm.9600007","type":"journal-article","created":{"date-parts":[[2005,1,27]],"date-time":"2005-01-27T11:32:31Z","timestamp":1106825551000},"page":"67-74","source":"Crossref","is-referenced-by-count":49,"title":["Long-Term Neuroprotection with 2-Iminobiotin, An Inhibitor of Neuronal and Inducible Nitric Oxide Synthase, after Cerebral Hypoxia-Ischemia in Neonatal Rats"],"prefix":"10.1177","volume":"25","author":[{"given":"Evelyn RW van den","family":"Tweel","sequence":"first","affiliation":[{"name":"Department of Neonatology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, The Netherlands"},{"name":"Laboratory for Psycho-Neuro-Immunology and Perinatology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"van Bel","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annemieke","family":"Kavelaars","sequence":"additional","affiliation":[{"name":"Laboratory for Psycho-Neuro-Immunology and Perinatology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cacha MPCD","family":"Peeters-Scholte","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johan","family":"Haumann","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cora HA","family":"Nijboer","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, The Netherlands"},{"name":"Laboratory for Psycho-Neuro-Immunology and Perinatology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cobi J","family":"Heijnen","sequence":"additional","affiliation":[{"name":"Laboratory for Psycho-Neuro-Immunology and Perinatology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Floris","family":"Groenendaal","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Wilhelmina Children's 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