{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T03:30:08Z","timestamp":1770348608981,"version":"3.49.0"},"reference-count":48,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2007,8,1]],"date-time":"2007-08-01T00:00:00Z","timestamp":1185926400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,10,7]],"date-time":"2016-10-07T00:00:00Z","timestamp":1475798400000},"content-version":"vor","delay-in-days":3355,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Therapy"],"published-print":{"date-parts":[[2007,8]]},"DOI":"10.1038\/sj.mt.6300192","type":"journal-article","created":{"date-parts":[[2007,5,23]],"date-time":"2007-05-23T15:13:27Z","timestamp":1179933207000},"page":"1543-1550","source":"Crossref","is-referenced-by-count":66,"title":["Mesenchymal Stem Cell-mediated Gene Delivery of Bone Morphogenetic Protein-2 in an Articular Fracture Model"],"prefix":"10.1016","volume":"15","author":[{"given":"Terri","family":"Zachos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alisha","family":"Diggs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Weisbrode","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey","family":"Bartlett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alicia","family":"Bertone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1038\/sj.mt.6300192_bib1","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1097\/01.TA.0000051934.96670.37","article-title":"The death of articular chondrocytes after intra-articular fracture in humans","volume":"56","author":"Murray","year":"2004","journal-title":"J Trauma"},{"key":"10.1038\/sj.mt.6300192_bib2","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1302\/0301-620X.74B3.1587902","article-title":"Experimentally produced fractures of articular cartilage and bone. The effects of shear forces on the pig knee","volume":"74","author":"Tomatsu","year":"1992","journal-title":"J Bone Joint Surg Br"},{"key":"10.1038\/sj.mt.6300192_bib3","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.2106\/00004623-199310000-00012","article-title":"Healing and remodeling of articular incongruities in a rabbit fracture model","volume":"75","author":"Llinas","year":"1993","journal-title":"J Bone Joint Surg Am"},{"key":"10.1038\/sj.mt.6300192_bib4","first-page":"49","article-title":"Femoral fracture repair and postoperative management in New Zealand white rabbits","volume":"41","author":"Reuter","year":"2002","journal-title":"Contemp Top Lab Anim Sci"},{"key":"10.1038\/sj.mt.6300192_bib5","doi-asserted-by":"crossref","DOI":"10.1080\/08941930701234687","article-title":"Rodent models for the study of articular fracture healing","author":"Zachos","year":"2007","journal-title":"J Investigative Surg"},{"key":"10.1038\/sj.mt.6300192_bib6","doi-asserted-by":"crossref","first-page":"4397","DOI":"10.1073\/pnas.052716199","article-title":"In vitro cartilage formation by human adult stem cells from bone marrow stroma defines the sequence of cellular and molecular events during chondrogenesis","volume":"99","author":"Sekiya","year":"2002","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/sj.mt.6300192_bib7","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.2106\/00004623-199812000-00004","article-title":"The chondrogenic potential of human bone-marrow-derived mesenchymal progenitor cells","volume":"80","author":"Yoo","year":"1998","journal-title":"J Bone Joint Surg Am"},{"key":"10.1038\/sj.mt.6300192_bib8","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1126\/science.284.5411.143","article-title":"Multilineage potential of adult human mesenchymal stem cells","volume":"284","author":"Pittenger","year":"1999","journal-title":"Science"},{"key":"10.1038\/sj.mt.6300192_bib9","doi-asserted-by":"crossref","first-page":"905","DOI":"10.2106\/00004623-199907000-00002","article-title":"The effect of regional gene therapy with bone morphogenetic protein-2-producing bone-marrow cells on the repair of segmental femoral defects in rats","volume":"81","author":"Lieberman","year":"1999","journal-title":"J Bone Joint Surg Am"},{"key":"10.1038\/sj.mt.6300192_bib10","first-page":"689","article-title":"Selection and amplification of a bone marrow cell population and its induction to the chondro-osteogenic lineage by rhOP-1: an in vitro and in vivo study","volume":"45","author":"Andrades","year":"2001","journal-title":"Int J Dev Biol"},{"key":"10.1038\/sj.mt.6300192_bib11","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1080\/03008200390229912","article-title":"A modified rhTGF-\u03b21 and rhBMP-2 are effective in initiating a chondro-osseous differentiation pathway in bone marrow cells cultured in vitro","volume":"44","author":"Andrades","year":"2003","journal-title":"Connect Tissue Res"},{"key":"10.1038\/sj.mt.6300192_bib12","doi-asserted-by":"crossref","first-page":"2949","DOI":"10.1242\/jcs.00527","article-title":"Formation of a chondro-osseous rudiment in micromass cultures of human bone-marrow stromal cells","volume":"116","author":"Muraglia","year":"2003","journal-title":"J Cell Sci"},{"key":"10.1038\/sj.mt.6300192_bib13","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1634\/stemcells.22-1-74","article-title":"Short-term BMP-2 expression is sufficient for in vivo osteochondral differentiation of mesenchymal stem cells","volume":"22","author":"No\u00ebl","year":"2004","journal-title":"Stem Cells"},{"key":"10.1038\/sj.mt.6300192_bib14","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1002\/(SICI)1521-2254(199903\/04)1:2<121::AID-JGM26>3.0.CO;2-J","article-title":"Engineered pluripotent mesenchymal cells integrate and differentiate in regenerating bone: a novel cell-mediated gene therapy","volume":"1","author":"Gazit","year":"1999","journal-title":"J Gene Med"},{"key":"10.1038\/sj.mt.6300192_bib15","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1038\/sj.gt.3302106","article-title":"Ex vivo gene therapy in autologous bone marrow stromal cells for tissue-engineered maxillofacial bone regeneration","volume":"10","author":"Chang","year":"2003","journal-title":"Gene Ther"},{"key":"10.1038\/sj.mt.6300192_bib16","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1006\/mthe.2001.0291","article-title":"Exogenously regulated stem cell-mediated gene therapy for bone regeneration","volume":"3","author":"Moutsatsos","year":"2001","journal-title":"Mol Ther"},{"key":"10.1038\/sj.mt.6300192_bib17","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1002\/jor.1100170108","article-title":"Gene therapy: adenovirus-mediated human bone morphogenetic protein-2 gene transfer induces mesenchymal progenitor cell proliferation and differentiation in vitro and bone formation in vivo","volume":"17","author":"Lou","year":"1999","journal-title":"J Orthop Res"},{"key":"10.1038\/sj.mt.6300192_bib18","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1002\/jor.20068","article-title":"Gene-mediated osteogenic differentiation of stem cells with bone morphogenetic proteins-2 or -6","volume":"24","author":"Zachos","year":"2006","journal-title":"J Orthop Res"},{"key":"10.1038\/sj.mt.6300192_bib19","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1111\/j.1525-1594.2006.00288.x","article-title":"Low-intensity ultrasound stimulation enhances chondrogenic differentiation in alginate culture of mesenchymal stem cells","volume":"30","author":"Lee","year":"2006","journal-title":"Artif Organs"},{"key":"10.1038\/sj.mt.6300192_bib20","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.joca.2005.02.006","article-title":"BMP-2 induces the expression of chondrocyte-specific genes in bovine synovium-derived progenitor cells cultured in three-dimensional alginate hydrogel","volume":"13","author":"Park","year":"2005","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1038\/sj.mt.6300192_bib21","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1002\/1097-4652(200010)185:1<98::AID-JCP9>3.0.CO;2-1","article-title":"Isolation, characterization, and chondrogenic potential of human bone marrow-derived multipotential stromal cells","volume":"185","author":"Majumdar","year":"2000","journal-title":"J Cell Physiol"},{"key":"10.1038\/sj.mt.6300192_bib22","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1002\/jcp.10025","article-title":"BMP-2 and BMP-9 promote chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1","volume":"189","author":"Majumdar","year":"2001","journal-title":"J Cell Physiol"},{"key":"10.1038\/sj.mt.6300192_bib23","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1002\/art.1780400605","article-title":"Bone morphogenetic protein 2 stimulates articular cartilage proteoglycan synthesis in vivo but does not counteract interleukin-1alpha effects on proteoglycan synthesis and content","volume":"40","author":"Glansbeek","year":"1997","journal-title":"Arthritis Rheum"},{"key":"10.1038\/sj.mt.6300192_bib24","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1002\/art.10759","article-title":"Articular cartilage repair by gene therapy using growth factor-producing mesenchymal cells","volume":"48","author":"Gelse","year":"2003","journal-title":"Arthritis Rheum"},{"key":"10.1038\/sj.mt.6300192_bib25","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1002\/jgm.826","article-title":"Transgene-activated mesenchymal cells for articular cartilage repair: a comparison of primary bone marrow-, perichondrium\/periosteum- and fat-derived cells","volume":"8","author":"Park","year":"2006","journal-title":"J Gene Med"},{"key":"10.1038\/sj.mt.6300192_bib26","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1290\/1071-2690(2002)038<0457:CDOHMS>2.0.CO;2","article-title":"Chondrogenic differentiation of human mesenchymal stem cells within an alginate layer culture system","volume":"38","author":"Kavalkovich","year":"2002","journal-title":"In Vitro Cell Dev Biol Anim"},{"key":"10.1038\/sj.mt.6300192_bib27","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1002\/jbm.a.10370","article-title":"Chondrogenesis of human mesenchymal stem cells encapsulated in alginate beads","volume":"64","author":"Ma","year":"2003","journal-title":"J Biomed Mater Res A"},{"key":"10.1038\/sj.mt.6300192_bib28","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1016\/S0142-9612(01)00329-5","article-title":"Formation of cartilage matrix proteins by BMP-transfected murine mesenchymal stem cells encapsulated in a novel class of alginates","volume":"23","author":"Weber","year":"2002","journal-title":"Biomaterials"},{"key":"10.1038\/sj.mt.6300192_bib29","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1016\/S0736-0266(03)00100-1","article-title":"Chondrogenic differentiation of mesenchymal progenitor cells encapsulated in ultrahigh-viscosity alginate","volume":"21","author":"Steinert","year":"2003","journal-title":"J Orthop Res"},{"key":"10.1038\/sj.mt.6300192_bib30","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1053\/jars.2000.4827","article-title":"Marrow stromal cells embedded in alginate for repair of osteochondral defects","volume":"16","author":"Diduch","year":"2000","journal-title":"Arthroscopy"},{"key":"10.1038\/sj.mt.6300192_bib31","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1002\/pat.231","article-title":"Polymer-encapsulated engineered adult mesenchymal stem cells secrete exogenously regulated rhBMP-2, and induce osteogenic and angiogenic tissue formation","volume":"13","author":"Zilberman","year":"2002","journal-title":"Polym Adv Technol"},{"key":"10.1038\/sj.mt.6300192_bib32","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/j.bone.2004.02.027","article-title":"Dual growth factor delivery and controlled scaffold degradation enhance in vivo bone formation by transplanted bone marrow stromal cells","volume":"35","author":"Simmons","year":"2004","journal-title":"Bone"},{"key":"10.1038\/sj.mt.6300192_bib33","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.jss.2003.08.003","article-title":"Cranial repair using BMP-2 gene engineered bone marrow stromal cells","volume":"119","author":"Chang","year":"2004","journal-title":"J Surg Res"},{"key":"10.1038\/sj.mt.6300192_bib34","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1002\/jor.1100160309","article-title":"Regional gene therapy with a BMP-2-producing murine stromal cell line induces heterotopic and orthotopic bone formation in rodents","volume":"16","author":"Lieberman","year":"1998","journal-title":"J Orthop Res"},{"key":"10.1038\/sj.mt.6300192_bib35","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1006\/mthe.2002.0691","article-title":"Ectopic osteogenesis using adenoviral bone morphogenetic protein (BMP)-4 and BMP-6 gene transfer","volume":"6","author":"Jane","year":"2002","journal-title":"Mol Ther"},{"key":"10.1038\/sj.mt.6300192_bib36","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1002\/1521-2254(200105\/06)3:3<240::AID-JGM181>3.0.CO;2-A","article-title":"Engineered human mesenchymal stem cells: a novel platform for skeletal cell mediated gene therapy","volume":"3","author":"Turgeman","year":"2001","journal-title":"J Gene Med"},{"key":"10.1038\/sj.mt.6300192_bib37","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1002\/art.10759","article-title":"Articular cartilage repair by gene therapy using growth factor-producing mesenchymal cells","volume":"48","author":"Gelse","year":"2003","journal-title":"Arthritis Rheum"},{"key":"10.1038\/sj.mt.6300192_bib38","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/S1063-4584(03)00004-9","article-title":"Localization of bone morphogenetic protein-2 in human osteoarthritic cartilage and osteophyte","volume":"11","author":"Nakase","year":"2003","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1038\/sj.mt.6300192_bib39","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1016\/j.orthres.2004.03.014","article-title":"Adenoviral-mediated transfer of human BMP-6 gene accelerates healing in a rabbit ulnar osteotomy model","volume":"22","author":"Bertone","year":"2004","journal-title":"J Orthop Res"},{"key":"10.1038\/sj.mt.6300192_bib40","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1038\/sj.gt.3301166","article-title":"Genetic enhancement of fracture repair: healing of an experimental segmental defect by adenoviral transfer of the BMP-2 gene","volume":"7","author":"Baltzer","year":"2000","journal-title":"Gene Ther"},{"key":"10.1038\/sj.mt.6300192_bib41","doi-asserted-by":"crossref","first-page":"355","DOI":"10.2106\/JBJS.E.00464","article-title":"Direct percutaneous gene delivery to enhance healing of segmental bone defects","volume":"88","author":"Betz","year":"2006","journal-title":"J Bone Joint Surg Am"},{"key":"10.1038\/sj.mt.6300192_bib42","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1089\/hum.2006.17.507","article-title":"Direct adenoviral transfer of bone morphogenetic protein-2 cDNA enhances fracture healing in osteoporotic sheep","volume":"17","author":"Egermann","year":"2006","journal-title":"Hum Gene Ther"},{"key":"10.1038\/sj.mt.6300192_bib43","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/S0736-0266(03)00129-3","article-title":"Evaluation of Ad-BMP-2 for enhancing fracture healing in an infected defect fracture rabbit model","volume":"22","author":"Southwood","year":"2004","journal-title":"J Orthop Res"},{"key":"10.1038\/sj.mt.6300192_bib44","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1163\/156856298X00550","article-title":"Ectopic bone formation induced by biodegradable hydrogels incorporating bone morphogenetic protein","volume":"9","author":"Yamamoto","year":"1998","journal-title":"J Biomater Sci Polymer Ed"},{"key":"10.1038\/sj.mt.6300192_bib45","unstructured":"AT Zachos, 2006 Gene-augmented mesenchymal stem cells in bone repair PhD Dissertation, The Ohio State Univ. Ch. 4 81-90"},{"key":"10.1038\/sj.mt.6300192_bib46","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1002\/jbm.a.30152","article-title":"Carrier dependent cell differentiation of bone morphogenetic protein-2 induced osteogenesis and chondrogenesis during the early implantation stage in rats","volume":"71A","author":"Takita","year":"2004","journal-title":"J Biomed Mater Res"},{"key":"10.1038\/sj.mt.6300192_bib47","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1359\/jbmr.2003.18.3.570","article-title":"Molecular imaging of the skeleton: quantitative real-time bioluminescence monitoring gene expression in bone repair and development","volume":"18","author":"Iris","year":"2003","journal-title":"J Bone Miner Res"},{"key":"10.1038\/sj.mt.6300192_bib48","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/j.ymthe.2003.12.009","article-title":"Gene therapy platform for bone regeneration using an exogenously regulated, AAV-2-based gene expression system","volume":"9","author":"Gafni","year":"2004","journal-title":"Mol Ther"}],"container-title":["Molecular Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525001616324509?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525001616324509?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,3,11]],"date-time":"2021-03-11T03:52:24Z","timestamp":1615434744000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1525001616324509"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8]]},"references-count":48,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2007,8]]}},"alternative-id":["S1525001616324509"],"URL":"https:\/\/doi.org\/10.1038\/sj.mt.6300192","relation":{},"ISSN":["1525-0016"],"issn-type":[{"value":"1525-0016","type":"print"}],"subject":[],"published":{"date-parts":[[2007,8]]}}}