{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:18:09Z","timestamp":1777335489748,"version":"3.51.4"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"},{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/srep02104","type":"journal-article","created":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T09:09:48Z","timestamp":1372669788000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["In Vivo and Real-time Monitoring of Secondary Metabolites of Living Organisms by Mass Spectrometry"],"prefix":"10.1038","volume":"3","author":[{"given":"Bin","family":"Hu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-Cai","family":"Ye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong-Ping","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2013,7,1]]},"reference":[{"key":"BFsrep02104_CR1","doi-asserted-by":"publisher","first-page":"15287","DOI":"10.1073\/pnas.0911451107","volume":"107","author":"J Roepke","year":"2010","unstructured":"Roepke, J. et al. Vinca drug components accumulate exclusively in leaf exudates of Madagascar periwinkle. Proc Natl Acad Sci USA 107, 15287\u201315292 (2010).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep02104_CR2","doi-asserted-by":"publisher","first-page":"2141","DOI":"10.1126\/science.291.5511.2141","volume":"291","author":"A Kessler","year":"2001","unstructured":"Kessler, A. & Baldwin, I. T. Defensive function of herbivore-induced plant volatile emissions in nature. Science 291, 2141\u20132144 (2001).","journal-title":"Science"},{"key":"BFsrep02104_CR3","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1002\/mas.20063","volume":"25","author":"M Glinski","year":"2006","unstructured":"Glinski, M. & Weckwerth, W. The role of mass spectrometry in plant systems biology. Mass Spectrom Rev 25, 173\u2013214 (2006).","journal-title":"Mass Spectrom Rev"},{"key":"BFsrep02104_CR4","doi-asserted-by":"publisher","first-page":"3237","DOI":"10.1021\/cr100012c","volume":"110","author":"K Chughtai","year":"2010","unstructured":"Chughtai, K. & Heeren, R. M. A. Mass spectrometric imaging for biomedical tissue analysis. Chem Rev 110, 3237\u20133277 (2010).","journal-title":"Chem Rev"},{"key":"BFsrep02104_CR5","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1039\/B906712B","volume":"40","author":"WB Dunn","year":"2011","unstructured":"Dunn, W. B., Broadhurst, D. I., Atherton, H. J., Goodacre, R. & Griffin, J. L. Systems level studies of mammalian metabolomes: the roles of mass spectrometry and nuclear magnetic resonance spectroscopy. Chem Soc Rev 40, 387\u2013426 (2011).","journal-title":"Chem Soc Rev"},{"key":"BFsrep02104_CR6","doi-asserted-by":"publisher","first-page":"1566","DOI":"10.1126\/science.1119426","volume":"311","author":"RG Cooks","year":"2006","unstructured":"Cooks, R. G., Ouyang, Z., Takats, Z. & Wiseman, J. M. Ambient mass spectrometry. Science 311, 1566\u20131570 (2006).","journal-title":"Science"},{"key":"BFsrep02104_CR7","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1007\/s00216-010-3808-3","volume":"398","author":"RM Alberici","year":"2010","unstructured":"Alberici, R. M. et al. Ambient mass spectrometry: bringing MS into the \"real world\". Anal Bioanal Chem 398, 265\u2013294 (2010).","journal-title":"Anal Bioanal Chem"},{"key":"BFsrep02104_CR8","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1146\/annurev.anchem.111808.073702","volume":"3","author":"MZ Huang","year":"2010","unstructured":"Huang, M. Z., Yuan, C. H., Cheng, S. C., Cho, Y. T. & Shiea, J. Ambient Ionization Mass Spectrometry. Annu Rev Anal Chem 3, 43\u201365 (2010).","journal-title":"Annu Rev Anal Chem"},{"key":"BFsrep02104_CR9","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1002\/mas.20346","volume":"31","author":"ZP Yao","year":"2012","unstructured":"Yao, Z. P. Characterization of proteins by ambient mass spectrometry. Mass Spectrom Rev 31, 437\u2013447 (2012).","journal-title":"Mass Spectrom Rev"},{"key":"BFsrep02104_CR10","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1126\/science.1104404","volume":"306","author":"Z Takats","year":"2004","unstructured":"Takats, Z., Wiseman, J. M., Gologan, B. & Cooks, R. G. Mass spectrometry sampling under ambient conditions with desorption electrospray ionization. Science 306, 471\u2013473 (2004).","journal-title":"Science"},{"key":"BFsrep02104_CR11","doi-asserted-by":"publisher","first-page":"1624","DOI":"10.1039\/b511161g","volume":"130","author":"RG Cooks","year":"2005","unstructured":"Cooks, R. G., Talaty, N. & Takats, Z. Rapid in situ detection of alkaloids in plant tissue under ambient conditions using desorption electrospray ionization. Analyst 130, 1624\u20131633 (2005).","journal-title":"Analyst"},{"key":"BFsrep02104_CR12","doi-asserted-by":"publisher","first-page":"7135","DOI":"10.1073\/pnas.0802692105","volume":"105","author":"JY Yew","year":"2008","unstructured":"Yew, J. Y., Cody, R. B. & Kravitz, E. A. Cuticular hydrocarbon analysis of an awake behaving fly using direct analysis in real-time time-of-flight mass spectrometry. Proc Natl Acad Sci USA 105, 7135\u20137140 (2008).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep02104_CR13","doi-asserted-by":"publisher","first-page":"7591","DOI":"10.1002\/anie.200702200","volume":"46","author":"H Chen","year":"2007","unstructured":"Chen, H., Yang, S., Wortmann, A. & Zenobi, R. Neutral desorption sampling of living objects for rapid analysis by extractive electrospray ionization mass spectrometry. Angew Chem Int Ed 46, 7591\u20137594 (2007).","journal-title":"Angew Chem Int Ed"},{"key":"BFsrep02104_CR14","doi-asserted-by":"publisher","first-page":"1467","DOI":"10.1038\/nprot.2008.109","volume":"3","author":"HW Chen","year":"2008","unstructured":"Chen, H. W. & Zenobi, R. Neutral desorption sampling of biological surfaces for rapid chemical characterization by extractive electrospray ionization mass spectrometry. Nat Protocols 3, 1467\u20131475 (2008).","journal-title":"Nat Protocols"},{"key":"BFsrep02104_CR15","doi-asserted-by":"publisher","first-page":"3613","DOI":"10.1039\/c2an16223g","volume":"137","author":"B Hu","year":"2012","unstructured":"Hu, B., Lai, Y. H., So, P. K., Chen, H. W. & Yao, Z. P. Direct ionization of biological tissue for mass spectrometric analysis. Analyst 137, 3613\u20133619 (2012).","journal-title":"Analyst"},{"key":"BFsrep02104_CR16","doi-asserted-by":"publisher","first-page":"7608","DOI":"10.1021\/ac2020273","volume":"83","author":"JJ Liu","year":"2011","unstructured":"Liu, J. J., Wang, H., Cooks, R. G. & Ouyang, Z. Leaf Spray: Direct Chemical Analysis of Plant Material and Living Plants by Mass Spectrometry. Anal Chem 83, 7608\u20137613 (2011).","journal-title":"Anal Chem"},{"key":"BFsrep02104_CR17","doi-asserted-by":"publisher","first-page":"2837","DOI":"10.1002\/rcm.5177","volume":"25","author":"SLF Chan","year":"2011","unstructured":"Chan, S. L. F., Wong, M. Y. M., Tang, H. W., Che, C. M. & Ng, K. M. Tissue-spray ionization mass spectrometry for raw herb analysis. Rapid Commun Mass Spectrom 25, 2837\u20132843 (2011).","journal-title":"Rapid Commun Mass Spectrom"},{"key":"BFsrep02104_CR18","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1111\/j.1432-1033.1997.00457.x","volume":"245","author":"R RomiLebrun","year":"1997","unstructured":"RomiLebrun, R. et al. Characterization of four toxins from Buthus martensi scorpion venom, which act on apamin-sensitive Ca2+-activated K+ channels. Eur J Biochem 245, 457\u2013464 (1997).","journal-title":"Eur J Biochem"},{"key":"BFsrep02104_CR19","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1046\/j.1432-1327.1999.00152.x","volume":"260","author":"O Hassani","year":"1999","unstructured":"Hassani, O. et al. Role of lysine and tryptophan residues in the biological activity of toxin VII (Ts gamma) from the scorpion Tityus serrulatus. Eur J Biochem 260, 76\u201386 (1999).","journal-title":"Eur J Biochem"},{"key":"BFsrep02104_CR20","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1006\/abbi.2001.2363","volume":"391","author":"SA Ali","year":"2001","unstructured":"Ali, S. A., Stoeva, S., Grossmann, J. G., Abbasi, A. & Voelter, W. Purification, characterization and primary structure of four depressant insect-selective neurotoxin analogs from scorpion (Buthus sindicus) venom. Arch Biochem Biophys 391, 197\u2013206 (2001).","journal-title":"Arch Biochem Biophys"},{"key":"BFsrep02104_CR21","doi-asserted-by":"publisher","first-page":"683","DOI":"10.1007\/s00359-004-0526-3","volume":"190","author":"A Weisel-Eichler","year":"2004","unstructured":"Weisel-Eichler, A. & Libersat, F. Venom effects on monoaminergic systems. J Comp Physiol A -Neuroethol Sens Neural Behav Physiol 190, 683\u2013690 (2004).","journal-title":"J Comp Physiol A -Neuroethol Sens Neural Behav Physiol"},{"key":"BFsrep02104_CR22","doi-asserted-by":"publisher","first-page":"1745","DOI":"10.1016\/j.peptides.2006.01.012","volume":"27","author":"XC Zeng","year":"2006","unstructured":"Zeng, X. C., Luo, F. & Li, W. X. Molecular dissection of venom from Chinese scorpion Mesobuthus martensii: Identification and characterization of four novel disulfide-bridged venom peptides. Peptides 27, 1745\u20131754 (2006).","journal-title":"Peptides"},{"key":"BFsrep02104_CR23","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/0041-0101(76)90098-2","volume":"14","author":"ZA Marie","year":"1976","unstructured":"Marie, Z. A. & Ibrahim, S. A. Lipid-content of scorpion (Leiurus-Quinquestriatus, H and E) venom. Toxicon 14, 93\u2013966 (1976).","journal-title":"Toxicon"},{"key":"BFsrep02104_CR24","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/0020-1790(77)90061-0","volume":"7","author":"NF Hadley","year":"1977","unstructured":"Hadley, N. F. & Jackson, L. L. Chemical composition of epicuticular lipids of scorpion, Paruroctonus-Mesaensis. Insect Biochem 7, 85\u201389 (1977).","journal-title":"Insect Biochem"},{"key":"BFsrep02104_CR25","first-page":"101","volume":"234","author":"G Delfino","year":"1995","unstructured":"Delfino, G., Brizzi, R. & Feri, L. Chemical skin defence in Bufo bufo: An ultrastructural study during ontogenesis. Zool Anz 234, 101\u2013111 (1995).","journal-title":"Zool Anz"},{"key":"BFsrep02104_CR26","doi-asserted-by":"publisher","first-page":"3058","DOI":"10.1021\/ac300748h","volume":"84","author":"YE Peng","year":"2012","unstructured":"Peng, Y. E. et al. In vivo nanoelectrospray for the localization of bioactive molecules in plants by mass spectrometry. Anal Chem 84, 3058\u20133062 (2012).","journal-title":"Anal Chem"},{"key":"BFsrep02104_CR27","doi-asserted-by":"publisher","first-page":"10989","DOI":"10.1002\/chem.201000847","volume":"16","author":"HY Tian","year":"2010","unstructured":"Tian, H. Y. et al. Bufogargarizins A and B: two novel 19-norbufadienolides with unprecedented skeletons from the venom of Bufo bufo gargarizans. Chem-Eur J 16, 10989\u201310993 (2010).","journal-title":"Chem-Eur J"},{"key":"BFsrep02104_CR28","doi-asserted-by":"publisher","first-page":"953","DOI":"10.1039\/c0np00032a","volume":"28","author":"HM Gao","year":"2011","unstructured":"Gao, H. M., Popescu, R., Kopp, B. & Wang, Z. M. Bufadienolides and their antitumor activity. Nat Prod Rep 28, 953\u2013969 (2011).","journal-title":"Nat Prod Rep"},{"key":"BFsrep02104_CR29","doi-asserted-by":"publisher","first-page":"2137","DOI":"10.1021\/ja0177049","volume":"124","author":"T Fukuyama","year":"2002","unstructured":"Fukuyama, T. et al. Stereocontrolled total synthesis of (+)-vinblastine. J Am Chem Soc 124, 2137\u20132139 (2002).","journal-title":"J Am Chem Soc"},{"key":"BFsrep02104_CR30","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1051\/agro:2008021","volume":"29","author":"M Farooq","year":"2009","unstructured":"Farooq, M., Wahid, A., Kobayashi, N., Fujita, D. & Basra, S. M. A. Plant drought stress: effects, mechanisms and management. Agron Sustain Dev 29, 185\u2013212 (2009).","journal-title":"Agron Sustain Dev"},{"key":"BFsrep02104_CR31","first-page":"1","volume":"2","author":"A Ibrahim","year":"2011","unstructured":"Ibrahim, A. et al. The alphabet of galactolipids in Arabidopsis thaliana. Plant Sci 2, 1\u201324 (2011).","journal-title":"Plant Sci"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep02104","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep02104.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep02104.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T05:15:12Z","timestamp":1672982112000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep02104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,1]]},"references-count":31,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2013,12,20]]}},"alternative-id":["BFsrep02104"],"URL":"https:\/\/doi.org\/10.1038\/srep02104","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,1]]},"assertion":[{"value":"17 April 2013","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 June 2013","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 July 2013","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"2104"}}