{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T13:57:22Z","timestamp":1758981442841},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2013,10,21]],"date-time":"2013-10-21T00:00:00Z","timestamp":1382313600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"},{"start":{"date-parts":[[2013,10,21]],"date-time":"2013-10-21T00:00:00Z","timestamp":1382313600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/srep03003","type":"journal-article","created":{"date-parts":[[2013,10,21]],"date-time":"2013-10-21T09:02:32Z","timestamp":1382346152000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Single domain intrabodies against WASP inhibit TCR-induced immune responses in transgenic mice T cells"],"prefix":"10.1038","volume":"3","author":[{"given":"Mitsuru","family":"Sato","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryoko","family":"Sawahata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chisato","family":"Sakuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takato","family":"Takenouchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Kitani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2013,10,21]]},"reference":[{"key":"BFsrep03003_CR1","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1126\/science.3140379","volume":"242","author":"RE Bird","year":"1988","unstructured":"Bird, R. E. et al. Single-chain antigen-binding proteins. Science 242, 423\u2013426 (1988).","journal-title":"Science"},{"key":"BFsrep03003_CR2","doi-asserted-by":"publisher","first-page":"5879","DOI":"10.1073\/pnas.85.16.5879","volume":"85","author":"JS Huston","year":"1988","unstructured":"Huston, J. S. et al. Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc Natl Acad Sci USA 85, 5879\u20135883 (1988).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep03003_CR3","doi-asserted-by":"publisher","first-page":"7889","DOI":"10.1073\/pnas.90.16.7889","volume":"90","author":"WA Marasco","year":"1993","unstructured":"Marasco, W. A., Haseltine, W. A. & Chen, S. Y. Design, intracellular expression and activity of a human anti-human immunodeficiency virus type 1 gp120 single-chain antibody. Proc Natl Acad Sci USA 90, 7889\u20137893 (1993).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep03003_CR4","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1073\/pnas.97.2.805","volume":"97","author":"P Steinberger","year":"2000","unstructured":"Steinberger, P., Andris-Widhopf, J., Buhler, B., Torbett, B. E. & Barbas, C. F. III Functional deletion of the CCR5 receptor by intracellular immunization produces cells that are refractory to CCR5-dependent HIV-1 infection and cell fusion. Proc Natl Acad Sci USA 97, 805\u2013810 (2000).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep03003_CR5","first-page":"3081","volume":"6","author":"RD Alvarez","year":"2000","unstructured":"Alvarez, R. D. et al. A cancer gene therapy approach utilizing an anti-erbB-2 single-chain antibody-encoding adenovirus (AD21): a phase I trial. Clin Cancer Res 6, 3081\u20133087 (2000).","journal-title":"Clin Cancer Res"},{"key":"BFsrep03003_CR6","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1093\/emboj\/cdg106","volume":"22","author":"T Tanaka","year":"2003","unstructured":"Tanaka, T. & Rabbitts, T. H. Intrabodies based on intracellular capture frameworks that bind the RAS protein with high affinity and impair oncogenic transformation. EMBO J 22, 1025\u20131035 (2003).","journal-title":"EMBO J"},{"key":"BFsrep03003_CR7","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1038\/sj.onc.1202338","volume":"18","author":"C Caron de Fromentel","year":"1999","unstructured":"Caron de Fromentel, C. et al. Restoration of transcriptional activity of p53 mutants in human tumour cells by intracellular expression of anti-p53 single chain Fv fragments. Oncogene 18, 551\u2013557 (1999).","journal-title":"Oncogene"},{"key":"BFsrep03003_CR8","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/S0167-7799(98)01268-2","volume":"17","author":"A Cattaneo","year":"1999","unstructured":"Cattaneo, A. & Biocca, S. The selection of intracellular antibodies. Trends Biotechnol 17, 115\u2013121 (1999).","journal-title":"Trends Biotechnol"},{"key":"BFsrep03003_CR9","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1016\/S1471-4914(03)00163-1","volume":"9","author":"MN Lobato","year":"2003","unstructured":"Lobato, M. N. & Rabbitts, T. H. Intracellular antibodies and challenges facing their use as therapeutic agents. Trends Mol Med 9, 390\u2013396 (2003).","journal-title":"Trends Mol Med"},{"key":"BFsrep03003_CR10","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1038\/363446a0","volume":"363","author":"C Hamers-Casterman","year":"1993","unstructured":"Hamers-Casterman, C. et al. Naturally occurring antibodies devoid of light chains. Nature 363, 446\u2013448 (1993).","journal-title":"Nature"},{"key":"BFsrep03003_CR11","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1016\/j.tibtech.2003.08.007","volume":"21","author":"LJ Holt","year":"2003","unstructured":"Holt, L. J., Herring, C., Jespers, L. S., Woolven, B. P. & Tomlinson, I. M. Domain antibodies: proteins for therapy. Trends Biotechnol 21, 484\u2013490 (2003).","journal-title":"Trends Biotechnol"},{"key":"BFsrep03003_CR12","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1182\/blood.V55.2.243.243","volume":"55","author":"HD Ochs","year":"1980","unstructured":"Ochs, H. D. et al. The Wiskott\u2013Aldrich syndrome: studies of lymphocytes, granulocytes and platelets. Blood 55, 243\u2013252 (1980).","journal-title":"Blood"},{"key":"BFsrep03003_CR13","doi-asserted-by":"publisher","first-page":"2621","DOI":"10.1182\/blood.V87.7.2621.bloodjournal8772621","volume":"87","author":"E Remold-O'Donnell","year":"1996","unstructured":"Remold-O'Donnell, E., Rosen, F. S. & Kenney, D. M. Defects in Wiskott\u2013Aldrich syndrome blood cells. Blood 87, 2621\u20132631 (1996).","journal-title":"Blood"},{"key":"BFsrep03003_CR14","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/S1074-7613(00)80590-7","volume":"9","author":"SB Snapper","year":"1998","unstructured":"Snapper, S. B. et al. Wiskott\u2013Aldrich syndrome protein-deficient mice reveal a role for WASP in T but not B cell activation. Immunity 9, 81\u201391 (1998).","journal-title":"Immunity"},{"key":"BFsrep03003_CR15","doi-asserted-by":"publisher","first-page":"1329","DOI":"10.1084\/jem.190.9.1329","volume":"190","author":"J Zhang","year":"1999","unstructured":"Zhang, J. et al. Antigen receptor-induced activation and cytoskeletal rearrangement are impaired in Wiskott\u2013Aldrich syndrome protein-deficient lymphocytes. J Exp Med 190, 1329\u20131342 (1999).","journal-title":"J Exp Med"},{"key":"BFsrep03003_CR16","doi-asserted-by":"crossref","first-page":"4383","DOI":"10.4049\/jimmunol.151.8.4383","volume":"151","author":"IJ Molina","year":"1993","unstructured":"Molina, I. J., Sancho, J., Terhorst, C., Rosen, F. S. & Remold-O'Donnell, E. T cells of patients with the Wiskott\u2013Aldrich syndrome have a restricted defect in proliferative responses. J Immunol 151, 4383\u20134390 (1993).","journal-title":"J Immunol"},{"key":"BFsrep03003_CR17","doi-asserted-by":"publisher","first-page":"2680","DOI":"10.1182\/blood.V90.7.2680","volume":"90","author":"Q Zhu","year":"1997","unstructured":"Zhu, Q. et al. Wiskott\u2013Aldrich syndrome\/X-linked thrombocytopenia: WASP gene mutations, protein expression and phenotype. Blood 90, 2680\u20132689 (1997).","journal-title":"Blood"},{"key":"BFsrep03003_CR18","doi-asserted-by":"publisher","first-page":"4701","DOI":"10.4049\/jimmunol.167.8.4701","volume":"167","author":"M Sato","year":"2001","unstructured":"Sato, M. et al. Overexpression of the Wiskott\u2013Aldrich syndrome protein N-terminal domain in transgenic mice inhibits T cell proliferative responses via TCR signaling without affecting cytoskeletal rearrangements. J Immunol 167, 4701\u20134709 (2001).","journal-title":"J Immunol"},{"key":"BFsrep03003_CR19","doi-asserted-by":"publisher","first-page":"6131","DOI":"10.1111\/j.1742-4658.2005.05011.x","volume":"272","author":"M Sato","year":"2005","unstructured":"Sato, M. et al. Intrabodies against the EVH1 domain of Wiskott\u2013Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells. FEBS J 272, 6131\u20136144 (2005).","journal-title":"FEBS J"},{"key":"BFsrep03003_CR20","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.yexmp.2008.11.005","volume":"86","author":"R Sawahata","year":"2009","unstructured":"Sawahata, R., Sato, M. & Kitani, H. Cytoplasmic expression and specific binding of the VH\/VL single domain intrabodies in transfected NIH3T3 cells. Exp Mol Pathol 86, 51\u201356 (2009).","journal-title":"Exp Mol Pathol"},{"key":"BFsrep03003_CR21","doi-asserted-by":"publisher","first-page":"5326","DOI":"10.1002\/j.1460-2075.1996.tb00917.x","volume":"15","author":"H Miki","year":"1996","unstructured":"Miki, H., Miura, K. & Takenawa, T. N-WASP, a novel actin-polymerizing protein, regulates the cortical cytoskeletal rearrangement in PIP2-dependent manner downstream of tyrosine kinases. EMBO J. 15, 5326\u20135335 (1996).","journal-title":"EMBO J."},{"key":"BFsrep03003_CR22","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1093\/intimm\/dxr042","volume":"23","author":"M Sato","year":"2011","unstructured":"Sato, M., Sawahata, R., Takenouchi, T. & Kitani, H. Identification of Fyn as the binding partner for the WASP N-terminal domain in T cells. Int Immunol 23, 493\u2013502 (2011).","journal-title":"Int Immunol"},{"key":"BFsrep03003_CR23","doi-asserted-by":"publisher","first-page":"14671","DOI":"10.1073\/pnas.94.26.14671","volume":"94","author":"N Ramesh","year":"1997","unstructured":"Ramesh, N., Anton, I. M., Hartwig, J. H. & Geha, R. S. WIP, a protein associated with Wiskott\u2013Aldrich syndrome protein, induces actin polymerization and redistribution in lymphoid cells. Proc Natl Acad Sci USA 94, 14671\u201314676 (1997).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep03003_CR24","doi-asserted-by":"publisher","first-page":"926","DOI":"10.1073\/pnas.0610275104","volume":"104","author":"MA de la Fuente","year":"2007","unstructured":"de la Fuente, M. A. et al. WIP is a chaperone for Wiskott\u2013Aldrich syndrome protein (WASP). Proc Natl Acad Sci USA 104, 926\u2013931 (2007).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep03003_CR25","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1016\/0952-7915(95)80107-3","volume":"7","author":"J Jain","year":"1995","unstructured":"Jain, J., Loh, C. & Rao, A. Transcriptional regulation of the IL-2 gene. Curr Opin Immunol 7, 333\u2013342 (1995).","journal-title":"Curr Opin Immunol"},{"key":"BFsrep03003_CR26","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1146\/annurev.immunol.15.1.707","volume":"15","author":"A Rao","year":"1997","unstructured":"Rao, A., Luo, C. & Hogan, P. G. Transcriptional factors of the NFAT family: regulation and function. Annu Rev Immunol 15, 707\u2013747 (1997).","journal-title":"Annu Rev Immunol"},{"key":"BFsrep03003_CR27","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1016\/j.jmb.2007.11.085","volume":"376","author":"T Tanaka","year":"2008","unstructured":"Tanaka, T. & Rabbits, T. H. Functional intracellular antibody fragments do not require invariant intra-domain disulfide bonds. J Mol Biol 376, 749\u2013757 (2008).","journal-title":"J Mol Biol"},{"key":"BFsrep03003_CR28","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1016\/0092-8674(86)90746-4","volume":"46","author":"S Munro","year":"1986","unstructured":"Munro, S. & Pelham, H. R. An Hsp70-like protein in the ER: identity with the 78\u2005kd glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell 46, 291\u2013300 (1986).","journal-title":"Cell"},{"key":"BFsrep03003_CR29","doi-asserted-by":"publisher","first-page":"726","DOI":"10.1038\/353726a0","volume":"353","author":"GC Flynn","year":"1991","unstructured":"Flynn, G. C., Pohl, J., Flocco, M. T. & Rothman, J. E. Peptide-binding specificity of the molecular chaperone Bip. Nature 353, 726\u2013730 (1991).","journal-title":"Nature"},{"key":"BFsrep03003_CR30","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1084\/jem.20030976","volume":"199","author":"K Badour","year":"2004","unstructured":"Badour, K. et al. Fyn and PTP\u2013PEST-mediated regulation of Wiskott\u2013Aldrich syndrome protein (WASp) tyrosine phosphorylation is required for coupling T cell antigen receptor engagement to WASp effector function and T cell activation. J Exp Med 199, 99\u2013112 (2004).","journal-title":"J Exp Med"},{"key":"BFsrep03003_CR31","doi-asserted-by":"publisher","first-page":"21450","DOI":"10.1074\/jbc.M010729200","volume":"276","author":"C Silvin","year":"2001","unstructured":"Silvin, C., Belisle, B. & Abo, A. A role for Wiskott\u2013Aldrich syndrome protein in T-cell receptor-mediated transcriptional activation independent of actin polymerization. J Biol Chem 276, 21450\u201321457 (2001).","journal-title":"J Biol Chem"},{"key":"BFsrep03003_CR32","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1016\/j.cbpa.2005.06.003","volume":"9","author":"M Stocks","year":"2005","unstructured":"Stocks, M. Intrabodies as drug discovery tools and therapeutics. Curr Opin Chem Biol 9, 359\u2013365 (2005).","journal-title":"Curr Opin Chem Biol"},{"key":"BFsrep03003_CR33","doi-asserted-by":"publisher","first-page":"1105","DOI":"10.1016\/j.mehy.2005.01.011","volume":"64","author":"BC Heng","year":"2005","unstructured":"Heng, B. C. & Cao, T. Making cell-permeable antibodies (Transbody) through fusion of protein transduction domain (PTD) with single chain variable fragment (scFv) antibodies: potential advantages over antibodies expressed within the intracellular environment (Intrabody). Med Hypotheses 64, 1105\u20131108 (2005).","journal-title":"Med Hypotheses"},{"key":"BFsrep03003_CR34","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/S0022-1759(01)00410-0","volume":"254","author":"Y Zhao","year":"2001","unstructured":"Zhao, Y., Lou, D., Burkett, J. & Kohler, H. Chemical engineering of cell penetrating antibodies. J Immunol Methods 254, 137\u2013145 (2001).","journal-title":"J Immunol Methods"},{"key":"BFsrep03003_CR35","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.ab.2005.06.011","volume":"344","author":"VV Didenko","year":"2005","unstructured":"Didenko, V. V., Ngo, H. & Baskin, D. S. Polyethyleneimine as a transmembrane carrier of fluorescently labeled proteins and antibodies. Anal Biochem 344, 168\u2013173 (2005).","journal-title":"Anal Biochem"},{"key":"BFsrep03003_CR36","doi-asserted-by":"publisher","first-page":"1493","DOI":"10.1021\/nn901586e","volume":"4","author":"SS Bale","year":"2010","unstructured":"Bale, S. S. et al. Nanoparticle-mediated cytoplasmic delivery of proteins to target cellular machinery. ACS NANO 4, 1493\u20131500 (2010).","journal-title":"ACS NANO"},{"key":"BFsrep03003_CR37","doi-asserted-by":"publisher","first-page":"3210","DOI":"10.1016\/j.biomaterials.2010.11.077","volume":"32","author":"DA Shah","year":"2011","unstructured":"Shah, D. A. et al. Regulation of stem cell signaling by nanoparticle-mediated intracellular protein delivery. Biomaterials 32, 3210\u20133219 (2011).","journal-title":"Biomaterials"},{"key":"BFsrep03003_CR38","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1084\/jem.158.2.303","volume":"158","author":"R Simonkevitz","year":"1983","unstructured":"Simonkevitz, R., Kappler, J., Marrack, P. & Grey, H. Antigen recognition by H-2-restricted T cells. I. Cell-free antigen processing. J Exp Med 158, 303\u2013316 (1983).","journal-title":"J Exp Med"},{"key":"BFsrep03003_CR39","doi-asserted-by":"publisher","first-page":"1699","DOI":"10.1093\/intimm\/8.11.1699","volume":"8","author":"Y Takemoto","year":"1996","unstructured":"Takemoto, Y., Sato, M., Furuta, M. & Hashimoto, Y. Distinct binding pattern of HS1 to the Src SH2 and SH3 domains reflect possible mechanisms of recruitment and activation of downstream molecules. Int Immunol 8, 1699\u20131705 (1996).","journal-title":"Int Immunol"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep03003","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep03003.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep03003.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T04:48:24Z","timestamp":1672980504000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep03003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10,21]]},"references-count":39,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2013,12,20]]}},"alternative-id":["BFsrep03003"],"URL":"https:\/\/doi.org\/10.1038\/srep03003","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,21]]},"assertion":[{"value":"21 May 2013","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 October 2013","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 October 2013","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"3003"}}