{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T01:02:32Z","timestamp":1789779752362,"version":"4.0.1"},"reference-count":18,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2013,11,15]],"date-time":"2013-11-15T00:00:00Z","timestamp":1384473600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"},{"start":{"date-parts":[[2013,11,15]],"date-time":"2013-11-15T00:00:00Z","timestamp":1384473600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/srep03224","type":"journal-article","created":{"date-parts":[[2013,11,15]],"date-time":"2013-11-15T10:45:56Z","timestamp":1384512356000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["Establishment of a Novel Lingual Organoid Culture System: Generation of Organoids Having Mature Keratinized Epithelium from Adult Epithelial Stem Cells"],"prefix":"10.1038","volume":"3","author":[{"given":"Hiroko","family":"Hisha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiro","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shohei","family":"Kanno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Tokuyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Komai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuichi","family":"Ohe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirotsugu","family":"Yanai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taichi","family":"Omachi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroo","family":"Ueno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,11,15]]},"reference":[{"key":"BFsrep03224_CR1","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1093\/chemse\/bjn025","volume":"33","author":"DJ Reiner","year":"2008","unstructured":"Reiner, D. J. et al. Genetic analysis of tongue size and taste papillae number and size in recombinant inbred strains of mice. Chem Senses 33, 693\u2013707 (2008).","journal-title":"Chem Senses"},{"key":"BFsrep03224_CR2","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1002\/(SICI)1096-9861(19970120)377:3<324::AID-CNE2>3.0.CO;2-4","volume":"377","author":"J-P Mbiene","year":"1997","unstructured":"Mbiene, J.-P., Maccallum, D. K. & Mistretta, C. M. Organ cultures of embryonic rat tongue support tongue and gustatory papilla morphogenesis in vitro without intact sensory ganglia. J Comp Neurol 377, 324\u2013340 (1997).","journal-title":"J Comp Neurol"},{"key":"BFsrep03224_CR3","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1016\/j.ydbio.2006.05.022","volume":"297","author":"Y Zhou","year":"2006","unstructured":"Zhou, Y., Liu, H.-X. & Mistretta, C. M. Bone morphogenic proteins and noggin: Inhibiting and inducing fungiform taste papilla development. Develop Biol 297, 198\u2013213 (2006).","journal-title":"Develop Biol"},{"key":"BFsrep03224_CR4","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1290\/1071-2690(2002)038<0365:FAEGFM>2.0.CO;2","volume":"38","author":"T Ookura","year":"2002","unstructured":"Ookura, T. et al. Fibroblast and epidermal growth factors modulate proliferation and neural cell adhesion molecule expression in epithelial cells derived from the adult mouse tongue. In Vitro Cell Dev Biol Anim 38, 365\u2013372 (2002).","journal-title":"In Vitro Cell Dev Biol Anim"},{"key":"BFsrep03224_CR5","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1007\/s11626-008-9149-2","volume":"45","author":"X Luo","year":"2009","unstructured":"Luo, X., Okubo, T., Randell, S. & Hogan, B. L. M. Culture of endodermal stem\/progenitor cells of the mouse tongue. In Vitro Cell Dev Biol Anim 45, 44\u201354 (2009).","journal-title":"In Vitro Cell Dev Biol Anim"},{"key":"BFsrep03224_CR6","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1038\/ncb2719","volume":"15","author":"T Tanaka","year":"2013","unstructured":"Tanaka, T. et al. Identification of stem cells that maintain and regenerate lingual keratinized epithelial cells. Nat Cell Biol 15, 511\u2013518 (2013).","journal-title":"Nat Cell Biol"},{"key":"BFsrep03224_CR7","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1038\/nature08873","volume":"464","author":"K Red-Horse","year":"2010","unstructured":"Red-Horse, K. et al. Coronary arteries form by developmental reprogramming of venous cells. Nature 464, 549\u2013553 (2010).","journal-title":"Nature"},{"key":"BFsrep03224_CR8","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1038\/nature10346","volume":"476","author":"Y Rinkevich","year":"2011","unstructured":"Rinkevich, Y. et al. Germ-layer and lineage-restricted stem\/progenitors regenerate the mouse digit tip. Nature 476, 409\u2013413 (2011).","journal-title":"Nature"},{"key":"BFsrep03224_CR9","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1038\/nature01587","volume":"423","author":"I-K Park","year":"2003","unstructured":"Park, I. -K. et al. Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells. Nature 423, 302\u2013305 (2003).","journal-title":"Nature"},{"key":"BFsrep03224_CR10","doi-asserted-by":"publisher","first-page":"962","DOI":"10.1038\/nature02060","volume":"425","author":"AV Molofsky","year":"2003","unstructured":"Molofsky, A. V. et al. Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation. Nature 425, 962\u2013967 (2003).","journal-title":"Nature"},{"key":"BFsrep03224_CR11","doi-asserted-by":"publisher","first-page":"2012","DOI":"10.1038\/nprot.2006.323","volume":"1","author":"A Dongari-Bagtzoglou","year":"2006","unstructured":"Dongari-Bagtzoglou, A. & Kashleva, H. Development of a highly reproducible three-demensional organotypic model of the oral mucosa. Nat Protoc 1, 2012\u20132018 (2006).","journal-title":"Nat Protoc"},{"key":"BFsrep03224_CR12","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1038\/nature09637","volume":"469","author":"T Sato","year":"2011","unstructured":"Sato, T. et al. Paneth cell constitute the niche for Lgr5 stem cells in intestinal crypts. Nature 469, 415\u2013418 (2011).","journal-title":"Nature"},{"key":"BFsrep03224_CR13","doi-asserted-by":"publisher","first-page":"618","DOI":"10.1038\/nm.2695","volume":"18","author":"S Yui","year":"2012","unstructured":"Yui, S. et al. Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5+ stem cell. Nat Med 18, 618\u2013623 (2012).","journal-title":"Nat Med"},{"key":"BFsrep03224_CR14","doi-asserted-by":"publisher","first-page":"404","DOI":"10.2353\/ajpath.2010.091079","volume":"177","author":"FT Schneider","year":"2010","unstructured":"Schneider, F. T. et al. Sonic hedgehog acts as a negative regulator of \u03b2-catenin signaling in the adult tongue epithelium. Am J Pathol 177, 404\u2013414 (2010).","journal-title":"Am J Pathol"},{"key":"BFsrep03224_CR15","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1042\/bj2800331","volume":"280","author":"N Flint","year":"1991","unstructured":"Flint, N. et al. A low-temperature method for the isolation of small-intestinal epithelium along the crypt-villus axis. Biochem J 280, 331\u2013334 (1991).","journal-title":"Biochem J"},{"key":"BFsrep03224_CR16","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1634\/stemcells.2008-0611","volume":"27","author":"T Okubo","year":"2009","unstructured":"Okubo, T. et al. Cell lineage mapping of taste bud cells and keratinocytes in the mouse tongue and soft palate. Stem Cells 27, 442\u2013450 (2009).","journal-title":"Stem Cells"},{"key":"BFsrep03224_CR17","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1158\/1078-0432.CCR-0999-3","volume":"10","author":"X-H Tang","year":"2004","unstructured":"Tang, X.-H. et al. Oral cavity and esophageal carcinogenesis modeled in carcinogen-treated mice. Clin Cancer Res 10, 301\u2013313 (2004).","journal-title":"Clin Cancer Res"},{"key":"BFsrep03224_CR18","doi-asserted-by":"publisher","first-page":"e126","DOI":"10.1016\/j.joms.2012.08.012","volume":"71","author":"J Takeuchi","year":"2013","unstructured":"Takeuchi, J. et al. Clinical evaluation of application of polyglycolic acid sheet and fibrin glue spray for partial glossectomy. J Oral Maxillofac Surg 71, e126\u2013e131 (2013).","journal-title":"J Oral Maxillofac Surg"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep03224","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep03224.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep03224.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T04:53:12Z","timestamp":1672980792000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep03224"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,15]]},"references-count":18,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2013,12,20]]}},"alternative-id":["BFsrep03224"],"URL":"https:\/\/doi.org\/10.1038\/srep03224","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,15]]},"assertion":[{"value":"28 August 2013","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 October 2013","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 November 2013","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"3224"}}