{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T05:32:08Z","timestamp":1785907928152,"version":"3.56.0"},"reference-count":88,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2014,9,2]],"date-time":"2014-09-02T00:00:00Z","timestamp":1409616000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"},{"start":{"date-parts":[[2014,9,2]],"date-time":"2014-09-02T00:00:00Z","timestamp":1409616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/srep06198","type":"journal-article","created":{"date-parts":[[2014,9,2]],"date-time":"2014-09-02T09:49:45Z","timestamp":1409651385000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["The vacuolar-sorting protein Snf7 is required for export of virulence determinants in members of the Cryptococcus neoformans complex."],"prefix":"10.1038","volume":"4","author":[{"given":"Rodrigo M.","family":"da C. Godinho","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Crestani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edvia","family":"Kmetzsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glauber","family":"de S. Araujo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Frases","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charley C.","family":"Staats","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augusto","family":"Schrank","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilene H.","family":"Vainstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcio L.","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2014,9,2]]},"reference":[{"key":"BFsrep06198_CR1","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1097\/QAD.0b013e328322ffac","volume":"23","author":"BJ Park","year":"2009","unstructured":"Park, B. J. et al. Estimation of the current global burden of cryptococcal meningitis among persons living with HIV\/AIDS. AIDS 23, 525\u201330 (2009).","journal-title":"AIDS"},{"key":"BFsrep06198_CR2","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1016\/j.idc.2006.07.001","volume":"20","author":"M Chayakulkeeree","year":"2006","unstructured":"Chayakulkeeree, M. & Perfect, J. R. Cryptococcosis. Infect Dis Clin North Am 20, 507\u201344, v-vi (2006).","journal-title":"Infect Dis Clin North Am"},{"key":"BFsrep06198_CR3","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1016\/j.micinf.2011.05.009","volume":"13","author":"EJ Byrnes 3rd","year":"2011","unstructured":"Byrnes, E. J., 3rd, Bartlett, K. H., Perfect, J. R. & Heitman, J. Cryptococcus gattii: an emerging fungal pathogen infecting humans and animals. Microbes Infect 13, 895\u2013907 (2011).","journal-title":"Microbes Infect"},{"key":"BFsrep06198_CR4","doi-asserted-by":"publisher","first-page":"667","DOI":"10.1016\/S1286-4579(03)00092-3","volume":"5","author":"JN Steenbergen","year":"2003","unstructured":"Steenbergen, J. N. & Casadevall, A. The origin and maintenance of virulence for the human pathogenic fungus Cryptococcus neoformans. Microbes Infect 5, 667\u201375 (2003).","journal-title":"Microbes Infect"},{"key":"BFsrep06198_CR5","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1128\/IAI.68.2.443-448.2000","volume":"68","author":"GM Cox","year":"2000","unstructured":"Cox, G. M., Mukherjee, J., Cole, G. T., Casadevall, A. & Perfect, J. R. Urease as a virulence factor in experimental cryptococcosis. Infect Immun 68, 443\u20138 (2000).","journal-title":"Infect Immun"},{"key":"BFsrep06198_CR6","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1038\/nrm2617","volume":"10","author":"W Nickel","year":"2009","unstructured":"Nickel, W. & Rabouille, C. Mechanisms of regulated unconventional protein secretion. Nat Rev Mol Cell Biol 10, 148\u201355 (2009).","journal-title":"Nat Rev Mol Cell Biol"},{"key":"BFsrep06198_CR7","doi-asserted-by":"publisher","first-page":"3781","DOI":"10.1091\/mbc.e10-05-0416","volume":"21","author":"R Schekman","year":"2010","unstructured":"Schekman, R. Charting the secretory pathway in a simple eukaryote. Mol Biol Cell 21, 3781\u20134 (2010).","journal-title":"Mol Biol Cell"},{"key":"BFsrep06198_CR8","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1016\/j.mib.2013.04.002","volume":"16","author":"ML Rodrigues","year":"2013","unstructured":"Rodrigues, M. L., Franzen, A. J., Nimrichter, L. & Miranda, K. Vesicular mechanisms of traffic of fungal molecules to the extracellular space. Curr Opin Microbiol 16, 414\u201320 (2013).","journal-title":"Curr Opin Microbiol"},{"key":"BFsrep06198_CR9","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1016\/j.tim.2008.12.005","volume":"17","author":"A Casadevall","year":"2009","unstructured":"Casadevall, A., Nosanchuk, J. D., Williamson, P. & Rodrigues, M. L. Vesicular transport across the fungal cell wall. Trends Microbiol 17, 158\u201362 (2009).","journal-title":"Trends Microbiol"},{"key":"BFsrep06198_CR10","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1080\/mmy.38.6.407.417","volume":"38","author":"A Vecchiarelli","year":"2000","unstructured":"Vecchiarelli, A. Immunoregulation by capsular components of Cryptococcus neoformans. Med Mycol 38, 407\u201317 (2000).","journal-title":"Med Mycol"},{"key":"BFsrep06198_CR11","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1128\/EC.2.4.655-663.2003","volume":"2","author":"I Bose","year":"2003","unstructured":"Bose, I., Reese, A. J., Ory, J. J., Janbon, G. & Doering, T. L. A yeast under cover: the capsule of Cryptococcus neoformans. Eukaryot Cell 2, 655\u201363 (2003).","journal-title":"Eukaryot Cell"},{"key":"BFsrep06198_CR12","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1111\/j.1574-695X.2011.00871.x","volume":"64","author":"R Garcia-Rodas","year":"2012","unstructured":"Garcia-Rodas, R. & Zaragoza, O. Catch me if you can: phagocytosis and killing avoidance by Cryptococcus neoformans. FEMS Immunol Med Microbiol 64, 147\u201361 (2012).","journal-title":"FEMS Immunol Med Microbiol"},{"key":"BFsrep06198_CR13","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/S0065-2164(09)01204-0","volume":"68","author":"O Zaragoza","year":"2009","unstructured":"Zaragoza, O. et al. The capsule of the fungal pathogen Cryptococcus neoformans. Adv Appl Microbiol 68, 133\u2013216 (2009).","journal-title":"Adv Appl Microbiol"},{"key":"BFsrep06198_CR14","doi-asserted-by":"publisher","first-page":"1274","DOI":"10.1111\/j.1462-5822.2008.01125.x","volume":"10","author":"SN Villena","year":"2008","unstructured":"Villena, S. N. et al. Capsular polysaccharides galactoxylomannan and glucuronoxylomannan from Cryptococcus neoformans induce macrophage apoptosis mediated by Fas ligand. Cell Microbiol 10, 1274\u201385 (2008).","journal-title":"Cell Microbiol"},{"key":"BFsrep06198_CR15","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1128\/EC.00318-06","volume":"6","author":"ML Rodrigues","year":"2007","unstructured":"Rodrigues, M. L. et al. Vesicular polysaccharide export in Cryptococcus neoformans is a eukaryotic solution to the problem of fungal trans-cell wall transport. Eukaryot Cell 6, 48\u201359 (2007).","journal-title":"Eukaryot Cell"},{"key":"BFsrep06198_CR16","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1007\/s11046-011-9468-9","volume":"173","author":"ML Rodrigues","year":"2012","unstructured":"Rodrigues, M. L. & Djordjevic, J. T. Unravelling secretion in Cryptococcus neoformans: more than one way to skin a cat. Mycopathologia 173, 407\u201318 (2012).","journal-title":"Mycopathologia"},{"key":"BFsrep06198_CR17","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1128\/EC.00370-07","volume":"7","author":"ML Rodrigues","year":"2008","unstructured":"Rodrigues, M. L. et al. Extracellular vesicles produced by Cryptococcus neoformans contain protein components associated with virulence. Eukaryot Cell 7, 58\u201367 (2008).","journal-title":"Eukaryot Cell"},{"key":"BFsrep06198_CR18","doi-asserted-by":"publisher","first-page":"9581","DOI":"10.3390\/ijms14059581","volume":"14","author":"D Oliveira","year":"2013","unstructured":"Oliveira, D., Rizzo, J., Joffe, L., Godinho, R. & Rodrigues, M. Where Do They Come from and Where Do They Go: Candidates for Regulating Extracellular Vesicle Formation in Fungi. Int J Mol Sci 14, 9581\u20139603 (2013).","journal-title":"Int J Mol Sci"},{"key":"BFsrep06198_CR19","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1042\/BJ20090957","volume":"424","author":"P Weiss","year":"2009","unstructured":"Weiss, P., Huppert, S. & Kolling, R. Analysis of the dual function of the ESCRT-III protein Snf7 in endocytic trafficking and in gene expression. Biochem J 424, 89\u201397 (2009).","journal-title":"Biochem J"},{"key":"BFsrep06198_CR20","doi-asserted-by":"publisher","first-page":"1059","DOI":"10.1091\/mbc.e09-07-0572","volume":"21","author":"A Shestakova","year":"2010","unstructured":"Shestakova, A. et al. Assembly of the AAA ATPase Vps4 on ESCRT-III. Mol Biol Cell 21, 1059\u201371 (2010).","journal-title":"Mol Biol Cell"},{"key":"BFsrep06198_CR21","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1042\/bj20031347","volume":"377","author":"JW Peck","year":"2004","unstructured":"Peck, J. W., Bowden, E. T. & Burbelo, P. D. Structure and function of human Vps20 and Snf7 proteins. Biochem J 377, 693\u2013700 (2004).","journal-title":"Biochem J"},{"key":"BFsrep06198_CR22","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1042\/BC20090145","volume":"102","author":"X Michelet","year":"2010","unstructured":"Michelet, X., Djeddi, A. & Legouis, R. Developmental and cellular functions of the ESCRT machinery in pluricellular organisms. Biol Cell 102, 191\u2013202 (2010).","journal-title":"Biol Cell"},{"key":"BFsrep06198_CR23","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1016\/j.ceb.2011.04.008","volume":"23","author":"M Babst","year":"2011","unstructured":"Babst, M. MVB vesicle formation: ESCRT-dependent, ESCRT-independent and everything in between. Curr Opin Cell Biol 23, 452\u20137 (2011).","journal-title":"Curr Opin Cell Biol"},{"key":"BFsrep06198_CR24","doi-asserted-by":"publisher","first-page":"1407","DOI":"10.1111\/j.1742-4658.2012.08548.x","volume":"279","author":"T Maeda","year":"2012","unstructured":"Maeda, T. The signaling mechanism of ambient pH sensing and adaptation in yeast and fungi. FEBS J 279, 1407\u201313 (2012).","journal-title":"FEBS J"},{"key":"BFsrep06198_CR25","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1534\/genetics.109.112029","volume":"184","author":"JM Wolf","year":"2010","unstructured":"Wolf, J. M. & Davis, D. A. Mutational analysis of Candida albicans SNF7 reveals genetically separable Rim101 and ESCRT functions and demonstrates divergence in bro1-domain protein interactions. Genetics 184, 673\u201394 (2010).","journal-title":"Genetics"},{"key":"BFsrep06198_CR26","first-page":"e01087","volume":"5","author":"Y Chen","year":"2014","unstructured":"Chen, Y. et al. The Cryptococcus neoformans Transcriptome at the Site of Human Meningitis. MBio 5, e01087\u201313 (2014).","journal-title":"MBio"},{"key":"BFsrep06198_CR27","doi-asserted-by":"publisher","first-page":"1651","DOI":"10.1172\/JCI27890","volume":"116","author":"PC Rittershaus","year":"2006","unstructured":"Rittershaus, P. C. et al. Glucosylceramide synthase is an essential regulator of pathogenicity of Cryptococcus neoformans. J Clin Invest 116, 1651\u20139 (2006).","journal-title":"J Clin Invest"},{"key":"BFsrep06198_CR28","doi-asserted-by":"publisher","first-page":"e12503","DOI":"10.1371\/journal.pone.0012503","volume":"5","author":"CD Chun","year":"2010","unstructured":"Chun, C. D. & Madhani, H. D. Ctr2 links copper homeostasis to polysaccharide capsule formation and phagocytosis inhibition in the human fungal pathogen Cryptococcus neoformans. PLoS One 5, e12503 (2010).","journal-title":"PLoS One"},{"key":"BFsrep06198_CR29","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1111\/j.1574-6968.2011.02386.x","volume":"324","author":"FD Silva","year":"2011","unstructured":"Silva, F. D. et al. Effects of microplusin, a copper-chelating antimicrobial peptide, against Cryptococcus neoformans. FEMS Microbiol Lett 324, 64\u201372 (2011).","journal-title":"FEMS Microbiol Lett"},{"key":"BFsrep06198_CR30","doi-asserted-by":"publisher","first-page":"1609","DOI":"10.1128\/EC.3.6.1609-1618.2004","volume":"3","author":"AL Kullas","year":"2004","unstructured":"Kullas, A. L., Li, M. & Davis, D. A. Snf7p, a component of the ESCRT-III protein complex, is an upstream member of the RIM101 pathway in Candida albicans. Eukaryot Cell 3, 1609\u201318 (2004).","journal-title":"Eukaryot Cell"},{"key":"BFsrep06198_CR31","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1534\/genetics.109.112029","volume":"184","author":"JM Wolf","year":"2010","unstructured":"Wolf, J. M. & Davis, D. A. Mutational analysis of Candida albicans SNF7 reveals genetically separable Rim101 and ESCRT functions and demonstrates divergence in bro1-domain protein interactions. Genetics 184, 673\u201394 (2010).","journal-title":"Genetics"},{"key":"BFsrep06198_CR32","doi-asserted-by":"publisher","first-page":"5528","DOI":"10.1091\/mbc.e04-08-0666","volume":"15","author":"W Xu","year":"2004","unstructured":"Xu, W., Smith, F. J., Jr, Subaran, R. & Mitchell, A. P. Multivesicular body-ESCRT components function in pH response regulation in Saccharomyces cerevisiae and Candida albicans. Mol Biol Cell 15, 5528\u201337 (2004).","journal-title":"Mol Biol Cell"},{"key":"BFsrep06198_CR33","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1111\/j.1600-0854.2004.00169.x","volume":"5","author":"K Bowers","year":"2004","unstructured":"Bowers, K. et al. Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae. Traffic 5, 194\u2013210 (2004).","journal-title":"Traffic"},{"key":"BFsrep06198_CR34","doi-asserted-by":"publisher","first-page":"1850","DOI":"10.1074\/jbc.M008381200","volume":"276","author":"TM Lamb","year":"2001","unstructured":"Lamb, T. M., Xu, W., Diamond, A. & Mitchell, A. P. Alkaline response genes of Saccharomyces cerevisiae and their relationship to the RIM101 pathway. J Biol Chem 276, 1850\u20136 (2001).","journal-title":"J Biol Chem"},{"key":"BFsrep06198_CR35","doi-asserted-by":"publisher","first-page":"e1000776","DOI":"10.1371\/journal.ppat.1000776","volume":"6","author":"TR O'Meara","year":"2010","unstructured":"O'Meara, T. R. et al. Interaction of Cryptococcus neoformans Rim101 and protein kinase A regulates capsule. PLoS Pathog 6, e1000776 (2010).","journal-title":"PLoS Pathog"},{"key":"BFsrep06198_CR36","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1128\/iai.65.2.405-411.1997","volume":"65","author":"SC Chen","year":"1997","unstructured":"Chen, S. C. et al. Identification of extracellular phospholipase B, lysophospholipase and acyltransferase produced by Cryptococcus neoformans. Infect Immun 65, 405\u201311 (1997).","journal-title":"Infect Immun"},{"key":"BFsrep06198_CR37","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1046\/j.1365-2958.2001.02236.x","volume":"39","author":"GM Cox","year":"2001","unstructured":"Cox, G. M. et al. Extracellular phospholipase activity is a virulence factor for Cryptococcus neoformans. Mol Microbiol 39, 166\u201375 (2001).","journal-title":"Mol Microbiol"},{"key":"BFsrep06198_CR38","doi-asserted-by":"publisher","first-page":"67","DOI":"10.5941\/MYCO.2013.41.2.67","volume":"41","author":"M Park","year":"2013","unstructured":"Park, M., Do, E. & Jung, W. H. Lipolytic enzymes involved in the virulence of human pathogenic fungi. Mycobiology 41, 67\u201372 (2013).","journal-title":"Mycobiology"},{"key":"BFsrep06198_CR39","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1038\/nrmicro2522","volume":"9","author":"JW Kronstad","year":"2011","unstructured":"Kronstad, J. W. et al. Expanding fungal pathogenesis: Cryptococcus breaks out of the opportunistic box. Nat Rev Microbiol 9, 193\u2013203 (2011).","journal-title":"Nat Rev Microbiol"},{"key":"BFsrep06198_CR40","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1128\/CMR.00001-12","volume":"25","author":"TR O'Meara","year":"2012","unstructured":"O'Meara, T. R. & Alspaugh, J. A. The Cryptococcus neoformans capsule: a sword and a shield. Clin Microbiol Rev 25, 387\u2013408 (2012).","journal-title":"Clin Microbiol Rev"},{"key":"BFsrep06198_CR41","doi-asserted-by":"publisher","first-page":"1371","DOI":"10.2217\/fmb.11.112","volume":"6","author":"ML Rodrigues","year":"2011","unstructured":"Rodrigues, M. L. et al. Vesicular transport systems in fungi. Future Microbiol 6, 1371\u201381 (2011).","journal-title":"Future Microbiol"},{"key":"BFsrep06198_CR42","doi-asserted-by":"publisher","first-page":"1538","DOI":"10.1128\/IAI.71.3.1538-1547.2003","volume":"71","author":"MC Noverr","year":"2003","unstructured":"Noverr, M. C., Cox, G. M., Perfect, J. R. & Huffnagle, G. B. Role of PLB1 in pulmonary inflammation and cryptococcal eicosanoid production. Infect Immun 71, 1538\u201347 (2003).","journal-title":"Infect Immun"},{"key":"BFsrep06198_CR43","doi-asserted-by":"publisher","first-page":"2229","DOI":"10.1128\/IAI.72.4.2229-2239.2004","volume":"72","author":"R Santangelo","year":"2004","unstructured":"Santangelo, R. et al. Role of extracellular phospholipases and mononuclear phagocytes in dissemination of cryptococcosis in a murine model. Infect Immun 72, 2229\u201339 (2004).","journal-title":"Infect Immun"},{"key":"BFsrep06198_CR44","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1042\/BJ20041079","volume":"384","author":"LC Wright","year":"2004","unstructured":"Wright, L. C. et al. Cryptococcal phospholipases: a novel lysophospholipase discovered in the pathogenic fungus Cryptococcus gattii. Biochem J 384, 377\u201384 (2004).","journal-title":"Biochem J"},{"key":"BFsrep06198_CR45","doi-asserted-by":"publisher","first-page":"3860","DOI":"10.1099\/mic.0.032854-0","volume":"155","author":"HC Eisenman","year":"2009","unstructured":"Eisenman, H. C., Frases, S., Nicola, A. M., Rodrigues, M. L. & Casadevall, A. Vesicle-associated melanization in Cryptococcus neoformans. Microbiology 155, 3860\u20137 (2009).","journal-title":"Microbiology"},{"key":"BFsrep06198_CR46","doi-asserted-by":"publisher","first-page":"1569","DOI":"10.1016\/j.bbagen.2006.07.002","volume":"1760","author":"KM Turner","year":"2006","unstructured":"Turner, K. M., Wright, L. C., Sorrell, T. C. & Djordjevic, J. T. N-linked glycosylation sites affect secretion of cryptococcal phospholipase B1, irrespective of glycosylphosphatidylinositol anchoring. Biochim Biophys Acta 1760, 1569\u201379 (2006).","journal-title":"Biochim Biophys Acta"},{"key":"BFsrep06198_CR47","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1146\/annurev-cellbio-092910-154152","volume":"28","author":"PI Hanson","year":"2012","unstructured":"Hanson, P. I. & Cashikar, A. Multivesicular body morphogenesis. Annu Rev Cell Dev Biol 28, 337\u201362 (2012).","journal-title":"Annu Rev Cell Dev Biol"},{"key":"BFsrep06198_CR48","doi-asserted-by":"publisher","first-page":"1165","DOI":"10.1111\/j.1365-2958.2008.06588.x","volume":"71","author":"J Panepinto","year":"2009","unstructured":"Panepinto, J. et al. Sec6-dependent sorting of fungal extracellular exosomes and laccase of Cryptococcus neoformans. Mol Microbiol 71, 1165\u201376 (2009).","journal-title":"Mol Microbiol"},{"key":"BFsrep06198_CR49","doi-asserted-by":"publisher","first-page":"5131","DOI":"10.1091\/mbc.e06-08-0701","volume":"17","author":"A Yoneda","year":"2006","unstructured":"Yoneda, A. & Doering, T. L. A eukaryotic capsular polysaccharide is synthesized intracellularly and secreted via exocytosis. Mol Biol Cell 17, 5131\u201340 (2006).","journal-title":"Mol Biol Cell"},{"key":"BFsrep06198_CR50","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1128\/IAI.01037-12","volume":"81","author":"G Hu","year":"2013","unstructured":"Hu, G. et al. Cryptococcus neoformans requires the ESCRT protein Vps23 for iron acquisition from heme, for capsule formation and for virulence. Infect Immun 81, 292\u2013302 (2013).","journal-title":"Infect Immun"},{"key":"BFsrep06198_CR51","doi-asserted-by":"publisher","first-page":"e11113","DOI":"10.1371\/journal.pone.0011113","volume":"5","author":"DL Oliveira","year":"2010","unstructured":"Oliveira, D. L. et al. Characterization of yeast extracellular vesicles: evidence for the participation of different pathways of cellular traffic in vesicle biogenesis. PLoS One 5, e11113 (2010).","journal-title":"PLoS One"},{"key":"BFsrep06198_CR52","doi-asserted-by":"publisher","first-page":"1695","DOI":"10.1111\/j.1462-5822.2008.01160.x","volume":"10","author":"PC Albuquerque","year":"2008","unstructured":"Albuquerque, P. C. et al. Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes. Cell Microbiol 10, 1695\u2013710 (2008).","journal-title":"Cell Microbiol"},{"key":"BFsrep06198_CR53","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1083\/jcb.200911154","volume":"188","author":"JM Duran","year":"2010","unstructured":"Duran, J. M., Anjard, C., Stefan, C., Loomis, W. F. & Malhotra, V. Unconventional secretion of Acb1 is mediated by autophagosomes. J Cell Biol 188, 527\u201336 (2010).","journal-title":"J Cell Biol"},{"key":"BFsrep06198_CR54","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1083\/jcb.200911149","volume":"188","author":"R Manjithaya","year":"2010","unstructured":"Manjithaya, R., Anjard, C., Loomis, W. F. & Subramani, S. Unconventional secretion of Pichia pastoris Acb1 is dependent on GRASP protein, peroxisomal functions and autophagosome formation. J Cell Biol 188, 537\u201346 (2010).","journal-title":"J Cell Biol"},{"key":"BFsrep06198_CR55","doi-asserted-by":"publisher","first-page":"533","DOI":"10.4161\/cib.3.6.12756","volume":"3","author":"DL Oliveira","year":"2010","unstructured":"Oliveira, D. L. et al. Biogenesis of extracellular vesicles in yeast: Many questions with few answers. Commun Integr Biol 3, 533\u20135 (2010).","journal-title":"Commun Integr Biol"},{"key":"BFsrep06198_CR56","doi-asserted-by":"publisher","first-page":"5143","DOI":"10.1242\/jcs.128561","volume":"126","author":"T Burgoyne","year":"2013","unstructured":"Burgoyne, T. et al. Expression of OA1 limits the fusion of a subset of MVBs with lysosomes - a mechanism potentially involved in the initial biogenesis of melanosomes. J Cell Sci 126, 5143\u201352 (2013).","journal-title":"J Cell Sci"},{"key":"BFsrep06198_CR57","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1093\/pcp\/pct197","volume":"55","author":"SJ Kim","year":"2014","unstructured":"Kim, S. J. & Brandizzi, F. The Plant Secretory Pathway: An Essential Factory for Building the Plant Cell Wall. Plant Cell Physiol 55, 687\u2013693 (2014).","journal-title":"Plant Cell Physiol"},{"key":"BFsrep06198_CR58","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1111\/j.1365-2958.2011.07686.x","volume":"81","author":"L Kmetzsch","year":"2011","unstructured":"Kmetzsch, L. et al. Role for Golgi reassembly and stacking protein (GRASP) in polysaccharide secretion and fungal virulence. Mol Microbiol 81, 206\u201318 (2011).","journal-title":"Mol Microbiol"},{"key":"BFsrep06198_CR59","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1128\/EC.00289-09","volume":"9","author":"G Hu","year":"2010","unstructured":"Hu, G. & Kronstad, J. W. A putative P-type ATPase, Apt1, is involved in stress tolerance and virulence in Cryptococcus neoformans. Eukaryot Cell 9, 74\u201383 (2010).","journal-title":"Eukaryot Cell"},{"key":"BFsrep06198_CR60","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1128\/EC.00273-13","volume":"13","author":"J Rizzo","year":"2014","unstructured":"Rizzo, J. et al. Role of the Apt1 Protein in Polysaccharide Secretion by Cryptococcus neoformans. Eukaryot Cell 13, 715\u201326 (2014).","journal-title":"Eukaryot Cell"},{"key":"BFsrep06198_CR61","doi-asserted-by":"publisher","first-page":"3519","DOI":"10.1128\/AAC.00545-06","volume":"50","author":"JD Nosanchuk","year":"2006","unstructured":"Nosanchuk, J. D. & Casadevall, A. Impact of melanin on microbial virulence and clinical resistance to antimicrobial compounds. Antimicrob Agents Chemother 50, 3519\u201328 (2006).","journal-title":"Antimicrob Agents Chemother"},{"key":"BFsrep06198_CR62","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1093\/genetics\/135.1.17","volume":"135","author":"J Tu","year":"1993","unstructured":"Tu, J., Vallier, L. G. & Carlson, M. Molecular and genetic analysis of the SNF7 gene in Saccharomyces cerevisiae. Genetics 135, 17\u201323 (1993).","journal-title":"Genetics"},{"key":"BFsrep06198_CR63","doi-asserted-by":"publisher","first-page":"5528","DOI":"10.1091\/mbc.e04-08-0666","volume":"15","author":"W Xu","year":"2004","unstructured":"Xu, W., Smith, F. J., Subaran, R. & Mitchell, A. P. Multivesicular body-ESCRT components function in pH response regulation in Saccharomyces cerevisiae and Candida albicans. Mol Biol Cell 15, 5528\u201337 (2004).","journal-title":"Mol Biol Cell"},{"key":"BFsrep06198_CR64","doi-asserted-by":"publisher","first-page":"1609","DOI":"10.1128\/EC.3.6.1609-1618.2004","volume":"3","author":"AL Kullas","year":"2004","unstructured":"Kullas, A. L., Li, M. & Davis, D. A. Snf7p, a component of the ESCRT-III protein complex, is an upstream member of the RIM101 pathway in Candida albicans. Eukaryot Cell 3, 1609\u201318 (2004).","journal-title":"Eukaryot Cell"},{"key":"BFsrep06198_CR65","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1534\/genetics.105.044644","volume":"171","author":"J Tilburn","year":"2005","unstructured":"Tilburn, J., S\u00e1nchez-Ferrero, J. C., Reoyo, E., Arst, H. N. & Pe\u00f1alva, M. A. Mutational analysis of the pH signal transduction component PalC of Aspergillus nidulans supports distant similarity to BRO1 domain family members. Genetics 171, 393\u2013401 (2005).","journal-title":"Genetics"},{"key":"BFsrep06198_CR66","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1128\/MMBR.66.3.426-446.2002","volume":"66","author":"MA Pe\u00f1alva","year":"2002","unstructured":"Pe\u00f1alva, M. A. & Arst, H. N. Regulation of gene expression by ambient pH in filamentous fungi and yeasts. Microbiol Mol Biol Rev 66, 426\u201346, table of contents (2002).","journal-title":"Microbiol Mol Biol Rev"},{"key":"BFsrep06198_CR67","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1128\/EC.00273-13","volume":"13","author":"J Rizzo","year":"2014","unstructured":"Rizzo, J. et al. Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans. Eukaryot Cell 13, 715\u2013726 (2014).","journal-title":"Eukaryot Cell"},{"key":"BFsrep06198_CR68","doi-asserted-by":"crossref","first-page":"4169","DOI":"10.1128\/IAI.66.9.4169-4175.1998","volume":"66","author":"ES Jacobson","year":"1998","unstructured":"Jacobson, E. S., Goodner, A. P. & Nyhus, K. J. Ferrous iron uptake in Cryptococcus neoformans. Infect Immun 66, 4169\u201375 (1998).","journal-title":"Infect Immun"},{"key":"BFsrep06198_CR69","doi-asserted-by":"publisher","first-page":"1452","DOI":"10.1111\/j.1365-2958.2004.04474.x","volume":"55","author":"T Lian","year":"2005","unstructured":"Lian, T. et al. Iron-regulated transcription and capsule formation in the fungal pathogen Cryptococcus neoformans. Mol Microbiol 55, 1452\u201372 (2005).","journal-title":"Mol Microbiol"},{"key":"BFsrep06198_CR70","doi-asserted-by":"publisher","first-page":"1036","DOI":"10.1128\/EC.2.5.1036-1045.2003","volume":"2","author":"JA Fraser","year":"2003","unstructured":"Fraser, J. A., Subaran, R. L., Nichols, C. B. & Heitman, J. Recapitulation of the sexual cycle of the primary fungal pathogen Cryptococcus neoformans var. gattii: implications for an outbreak on Vancouver Island, Canada. Eukaryot Cell 2, 1036\u201345 (2003).","journal-title":"Eukaryot Cell"},{"key":"BFsrep06198_CR71","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1128\/EC.3.2.420-429.2004","volume":"3","author":"A Idnurm","year":"2004","unstructured":"Idnurm, A., Reedy, J. L., Nussbaum, J. C. & Heitman, J. Cryptococcus neoformans virulence gene discovery through insertional mutagenesis. Eukaryot Cell 3, 420\u20139 (2004).","journal-title":"Eukaryot Cell"},{"key":"BFsrep06198_CR72","doi-asserted-by":"publisher","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","volume":"23","author":"MA Larkin","year":"2007","unstructured":"Larkin, M. A. et al. Clustal W and Clustal X version 2.0. Bioinformatics 23, 2947\u20138 (2007).","journal-title":"Bioinformatics"},{"key":"BFsrep06198_CR73","doi-asserted-by":"publisher","first-page":"2731","DOI":"10.1093\/molbev\/msr121","volume":"28","author":"K Tamura","year":"2011","unstructured":"Tamura, K. et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Mol Biol Evol 28, 2731\u20139 (2011).","journal-title":"Mol Biol Evol"},{"key":"BFsrep06198_CR74","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1016\/j.fgb.2007.11.001","volume":"45","author":"MD Garc\u00eda-Pedrajas","year":"2008","unstructured":"Garc\u00eda-Pedrajas, M. D. et al. DelsGate, a robust and rapid gene deletion construction method. Fungal Genet Biol 45, 379\u201388 (2008).","journal-title":"Fungal Genet Biol"},{"key":"BFsrep06198_CR75","doi-asserted-by":"publisher","first-page":"1798","DOI":"10.1128\/EC.00114-10","volume":"Vol. 9","author":"L Kmetzsch","year":"2010","unstructured":"Kmetzsch, L. et al. The vacuolar Ca(2)(+) exchanger Vcx1 is involved in calcineurin-dependent Ca(2)(+) tolerance and virulence in Cryptococcus neoformans. in Eukaryot Cell, Vol. 9, 1798\u2013805 (2010).","journal-title":"Eukaryot Cell,"},{"key":"BFsrep06198_CR76","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1016\/j.fgb.2010.07.011","volume":"48","author":"L Kmetzsch","year":"2011","unstructured":"Kmetzsch, L. et al. The GATA-type transcriptional activator Gat1 regulates nitrogen uptake and metabolism in the human pathogen Cryptococcus neoformans. Fungal Genet Biol 48, 192\u20139 (2011).","journal-title":"Fungal Genet Biol"},{"key":"BFsrep06198_CR77","doi-asserted-by":"publisher","first-page":"e43773","DOI":"10.1371\/journal.pone.0043773","volume":"7","author":"O Schneider Rde","year":"2012","unstructured":"Schneider Rde, O. et al. Zap1 Regulates Zinc Homeostasis and Modulates Virulence in Cryptococcus gattii. PLoS One 7, e43773 (2012).","journal-title":"PLoS One"},{"key":"BFsrep06198_CR78","doi-asserted-by":"publisher","first-page":"1405","DOI":"10.1128\/jb.175.5.1405-1411.1993","volume":"175","author":"DL Toffaletti","year":"1993","unstructured":"Toffaletti, D. L., Rude, T. H., Johnston, S. A., Durack, D. T. & Perfect, J. R. Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA. J Bacteriol 175, 1405\u201311 (1993).","journal-title":"J Bacteriol"},{"key":"BFsrep06198_CR79","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1111\/j.1365-2958.2011.07686.x","volume":"81","author":"L Kmetzsch","year":"2011","unstructured":"Kmetzsch, L. et al. Role for Golgi reassembly and stacking protein (GRASP) in polysaccharide secretion and fungal virulence. Mol Microbiol 81, 206\u201318 (2011).","journal-title":"Mol Microbiol"},{"key":"BFsrep06198_CR80","doi-asserted-by":"publisher","first-page":"855","DOI":"10.1128\/EC.00399-06","volume":"6","author":"LG Baker","year":"2007","unstructured":"Baker, L. G., Specht, C. A., Donlin, M. J. & Lodge, J. K. Chitosan, the deacetylated form of chitin, is necessary for cell wall integrity in Cryptococcus neoformans. Eukaryot Cell 6, 855\u201367 (2007).","journal-title":"Eukaryot Cell"},{"key":"BFsrep06198_CR81","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1128\/EC.00307-07","volume":"7","author":"ML Rodrigues","year":"2008","unstructured":"Rodrigues, M. L., Alvarez, M., Fonseca, F. L. & Casadevall, A. Binding of the wheat germ lectin to Cryptococcus neoformans suggests an association of chitinlike structures with yeast budding and capsular glucuronoxylomannan. Eukaryot Cell 7, 602\u20139 (2008).","journal-title":"Eukaryot Cell"},{"key":"BFsrep06198_CR82","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/0022-1759(92)90209-C","volume":"154","author":"A Casadevall","year":"1992","unstructured":"Casadevall, A., Mukherjee, J. & Scharff, M. D. Monoclonal antibody based ELISAs for cryptococcal polysaccharide. J Immunol Methods 154, 27\u201335 (1992).","journal-title":"J Immunol Methods"},{"key":"BFsrep06198_CR83","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1016\/j.fgb.2009.03.003","volume":"46","author":"FL Fonseca","year":"2009","unstructured":"Fonseca, F. L. et al. Structural and functional properties of the Trichosporon asahii glucuronoxylomannan. Fungal Genet Biol 46, 496\u2013505 (2009).","journal-title":"Fungal Genet Biol"},{"key":"BFsrep06198_CR84","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1080\/00362178285380031","volume":"20","author":"MF Price","year":"1982","unstructured":"Price, M. F., Wilkinson, I. D. & Gentry, L. O. Plate method for detection of phospholipase activity in Candida albicans. Sabouraudia 20, 7\u201314 (1982).","journal-title":"Sabouraudia"},{"key":"BFsrep06198_CR85","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1093\/infdis\/175.2.414","volume":"175","author":"SC Chen","year":"1997","unstructured":"Chen, S. C., Muller, M., Zhou, J. Z., Wright, L. C. & Sorrell, T. C. Phospholipase activity in Cryptococcus neoformans: a new virulence factor? J Infect Dis 175, 414\u201320 (1997).","journal-title":"J Infect Dis"},{"key":"BFsrep06198_CR86","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1128\/iai.55.8.1751-1754.1987","volume":"55","author":"KJ Kwon-Chung","year":"1987","unstructured":"Kwon-Chung, K. J., Wickes, B. L., Booth, J. L., Vishniac, H. S. & Bennett, J. E. Urease inhibition by EDTA in the two varieties of Cryptococcus neoformans. Infect Immun 55, 1751\u20134 (1987).","journal-title":"Infect Immun"},{"key":"BFsrep06198_CR87","doi-asserted-by":"publisher","first-page":"249","DOI":"10.5941\/MYCO.2011.39.4.249","volume":"39","author":"K Selvig","year":"2011","unstructured":"Selvig, K. & Alspaugh, J. A. pH Response Pathways in Fungi: Adapting to Host-derived and Environmental Signals. Mycobiology 39, 249\u201356 (2011).","journal-title":"Mycobiology"},{"key":"BFsrep06198_CR88","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402\u20138 (2001).","journal-title":"Methods"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep06198","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep06198.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep06198.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T08:01:15Z","timestamp":1672992075000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep06198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,2]]},"references-count":88,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2014,12,17]]}},"alternative-id":["BFsrep06198"],"URL":"https:\/\/doi.org\/10.1038\/srep06198","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,2]]},"assertion":[{"value":"23 June 2014","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 August 2014","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 September 2014","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"6198"}}