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In this study, we investigated variations in NH<jats:sub>4<\/jats:sub><jats:sup>+<\/jats:sup> and NO<jats:sub>3<\/jats:sub><jats:sup>-<\/jats:sup> fluxes in the fine roots of wheat using a scanning ion-selective electrode technique in the presence of different nitrogen (N) forms, N concentrations and pH levels as well as under water stress. Our results show that the fine roots of wheat demonstrated maximum NH<jats:sub>4<\/jats:sub><jats:sup>+<\/jats:sup> and NO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> influxes at 20\u2005mm and 25\u2005mm from the root tip, respectively. The maximal net NH<jats:sub>4<\/jats:sub><jats:sup>+<\/jats:sup> and NO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> influxes were observed at pH 6.2 in the presence of a 1\/4\u2005N solution. We observed N efflux in two different cultivars following the exposure of roots to a 10% PEG-6000 solution. Furthermore, the drought-tolerant cultivar generally performed better than the drought-intolerant cultivar. Net NH<jats:sub>4<\/jats:sub><jats:sup>+<\/jats:sup> and NO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> fluxes may be determined by plant growth status, but environmental conditions can also affect the magnitude and direction of N flux. Interestingly, we found that NO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> was more sensitive to environmental changes than NH<jats:sub>4<\/jats:sub><jats:sup>+<\/jats:sup>. Our results may be used to guide future hydroponic experiments in wheat as well as to aid in the development of effective fertilisation protocols for this crop.<\/jats:p>","DOI":"10.1038\/srep07223","type":"journal-article","created":{"date-parts":[[2014,11,27]],"date-time":"2014-11-27T10:08:02Z","timestamp":1417082882000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Net ammonium and nitrate fluxes in wheat roots under different environmental conditions as assessed by scanning ion-selective electrode technique"],"prefix":"10.1038","volume":"4","author":[{"given":"Yangquanwei","family":"Zhong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiming","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhouping","family":"Shangguan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2014,11,27]]},"reference":[{"key":"BFsrep07223_CR1","doi-asserted-by":"publisher","first-page":"919","DOI":"10.1007\/s00425-012-1807-7","volume":"237","author":"J Luo","year":"2013","unstructured":"Luo, J. et al. 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