{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T20:36:40Z","timestamp":1767991000074,"version":"3.49.0"},"reference-count":58,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2015,2,2]],"date-time":"2015-02-02T00:00:00Z","timestamp":1422835200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2015,2,2]],"date-time":"2015-02-02T00:00:00Z","timestamp":1422835200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cell membrane proteins are believed to play a critical role in the pathogenesis of autoimmune diseases. However, few membrane autoantigens have been linked with Beh\u00e7et's disease. Here, a cell-chip was performed to identify autoantibody target cells and the suspected autoantigens were detected using immunoblotting. The amino acid sequences of the detected proteins were determined using LC-MALDI-TOF\/TOF. Putative proteins were recombinantly expressed and purified and a corresponding ELISA was developed and clinically validated using real clinical samples. It was found that a 36-kDa membrane protein - annexin A2 - was detected in approximately one-third of the patients' blood circulation. The immunohistochemistry results showed that annexin A2 was highly expressed in vascular endothelial cells. Moreover, vascular involvement was significantly higher in the anti-annexin A2 antibody-positive group versus the anti-annexin A2 antibody-negative group among all the clinical samples analyzed, indicating that annexin A2 is a novel endothelial cell membrane antigen involved in Beh\u00e7et's disease.<\/jats:p>","DOI":"10.1038\/srep08162","type":"journal-article","created":{"date-parts":[[2015,2,2]],"date-time":"2015-02-02T10:03:04Z","timestamp":1422871384000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Annexin A2 as a target endothelial cell membrane autoantigen in Beh\u00e7et's disease"],"prefix":"10.1038","volume":"5","author":[{"given":"Peng","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaping","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiping","family":"Xun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ran","family":"Bao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangyu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunhe","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shutao","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yabin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongqiang","family":"Wen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongzhong","family":"Bian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongwu","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,2,2]]},"reference":[{"key":"BFsrep08162_CR1","doi-asserted-by":"publisher","first-page":"1284","DOI":"10.1056\/NEJM199910213411707","volume":"341","author":"T Sakane","year":"1999","unstructured":"Sakane, T., Takeno, M., Suzuki, N. & Inaba, G. Beh\u00e7et's disease. New Engl. J. Med. 341, 1284\u20131291 (1999).","journal-title":"New Engl. J. Med."},{"key":"BFsrep08162_CR2","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1034\/j.1399-0039.1999.540301.x","volume":"54","author":"DH Verity","year":"1999","unstructured":"Verity, D. H. et al. Beh\u00e7et's disease, the Silk Road and HLA-B51: historical and geographical perspectives. Tissue Antigens 54, 213\u2013220 (1999).","journal-title":"Tissue Antigens"},{"key":"BFsrep08162_CR3","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1016\/j.autrev.2011.11.026","volume":"11","author":"M Pineton De Chambrun","year":"2012","unstructured":"Pineton De Chambrun, M. et al. New insights into the pathogenesis of Behcet's disease. Autoimmun. Rev. 11, 687\u2013698 (2012).","journal-title":"Autoimmun. Rev."},{"key":"BFsrep08162_CR4","first-page":"55","volume":"3","author":"S Hsieh","year":"2013","unstructured":"Hsieh, S. & Yu, C. Autoantibody profiling in systemic lupus erythematosus. Curr. Biomark. Find. 3, 55\u201356 (2013).","journal-title":"Curr. Biomark. Find."},{"key":"BFsrep08162_CR5","doi-asserted-by":"publisher","first-page":"1731","DOI":"10.1136\/ard.2005.035691","volume":"64","author":"V Nell","year":"2005","unstructured":"Nell, V. et al. Autoantibody profiling as early diagnostic and prognostic tool for rheumatoid arthritis. Ann. Rheum. Dis. 64, 1731\u20131736 (2005).","journal-title":"Ann. Rheum. Dis."},{"key":"BFsrep08162_CR6","doi-asserted-by":"publisher","first-page":"3067","DOI":"10.1167\/iovs.09-4313","volume":"51","author":"M Takeuchi","year":"2010","unstructured":"Takeuchi, M. et al. Immune responses to interphotoreceptor retinoid-binding protein and S-antigen in Behcet's patients with uveitis. Invest. Ophth. Vis. Sci. 51, 3067\u20133075 (2010).","journal-title":"Invest. Ophth. Vis. Sci."},{"key":"BFsrep08162_CR7","first-page":"S96","volume":"26","author":"E Birtas-Atesoglu","year":"2008","unstructured":"Birtas-Atesoglu, E. et al. Serum levels of free heat shock protein 70 and anti-HSP70 are elevated in Behcet's disease. Clin. Exp. Rheumatol. 26, S96\u2013S98 (2008).","journal-title":"Clin. Exp. Rheumatol."},{"key":"BFsrep08162_CR8","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1002\/1521-4141(200202)32:2<356::AID-IMMU356>3.0.CO;2-9","volume":"32","author":"F Mor","year":"2002","unstructured":"Mor, F., Weinberger, A. & Cohen, I. R. Identification of alpha-tropomyosin as a target self-antigen in Behcet's syndrome. Eur. J. Immunol. 32, 356\u2013365 (2002).","journal-title":"Eur. J. Immunol."},{"key":"BFsrep08162_CR9","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1016\/j.exer.2007.01.003","volume":"84","author":"Y Okunuki","year":"2007","unstructured":"Okunuki, Y. et al. Proteomic surveillance of autoimmunity in Behcet's disease with uveitis: selenium binding protein is a novel autoantigen in Behcet's disease. Exp. Eye Res. 84, 823\u2013831 (2007).","journal-title":"Exp. Eye Res."},{"key":"BFsrep08162_CR10","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.jneuroim.2013.08.007","volume":"263","author":"B Vural","year":"2013","unstructured":"Vural, B. et al. Mitochondrial carrier homolog 1 (Mtch1) antibodies in neuro-Beh\u00e7et's disease. J. Neuroimmunol. 263, 139\u2013144 (2013).","journal-title":"J. Neuroimmunol."},{"key":"BFsrep08162_CR11","doi-asserted-by":"publisher","first-page":"e124","DOI":"10.1111\/j.1756-185X.2012.01759.x","volume":"15","author":"AG Tamer","year":"2012","unstructured":"Tamer, A. G., Hatem, S. & Hani, H. Anti-annexin V antibodies in neuro- Beh\u00e7et patients: clinical significance and relation to disease activity. Int. J. Rheum. Dis. 15, e124\u2013e126 (2012).","journal-title":"Int. J. Rheum. Dis."},{"key":"BFsrep08162_CR12","first-page":"S27","volume":"21","author":"A Dinc","year":"2003","unstructured":"Dinc, A. et al. Anti-endothelial cell antibodies in Beh\u00e7et's disease. Clin. Exp. Rheumatol. 21, S27\u2013S30 (2003).","journal-title":"Clin. Exp. Rheumatol."},{"key":"BFsrep08162_CR13","first-page":"157","volume":"67","author":"AO Ayd\u0131ntug","year":"1993","unstructured":"Ayd\u0131ntug, A. O. et al. Antibodies to endothelial cells in patients with Beh\u00e7et's disease. Clin. Immunol. 67, 157\u2013162 (1993).","journal-title":"Clin. Immunol."},{"key":"BFsrep08162_CR14","doi-asserted-by":"publisher","first-page":"2025","DOI":"10.1002\/art.11074","volume":"48","author":"KH Lee","year":"2003","unstructured":"Lee, K. H. et al. Human \u03b1-enolase from endothelial cells as a target antigen of anti\u2013endothelial cell antibody in Beh\u00e7et's disease. Arthritis Rheumatol. 48, 2025\u20132035 (2003).","journal-title":"Arthritis Rheumatol."},{"key":"BFsrep08162_CR15","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1038\/jid.2011.397","volume":"132","author":"SB Cho","year":"2012","unstructured":"Cho, S. B. et al. Identification of HnRNP-A2\/B1 as a target antigen of anti-endothelial cell IgA antibody in Behcet's disease. J. Invest. Dermatol. 132, 601\u2013608 (2012).","journal-title":"J. Invest. Dermatol."},{"key":"BFsrep08162_CR16","doi-asserted-by":"publisher","first-page":"R71","DOI":"10.1186\/ar1940","volume":"8","author":"F Delunardo","year":"2006","unstructured":"Delunardo, F. et al. Identification and characterization of the carboxy-terminal region of Sip-1, a novel autoantigen in Beh\u00e7et's disease. Arthritis Res. Ther. 8, R71 (2006).","journal-title":"Arthritis Res. Ther."},{"key":"BFsrep08162_CR17","doi-asserted-by":"publisher","first-page":"4559","DOI":"10.1182\/blood-2007-05-092825","volume":"111","author":"P Margutti","year":"2008","unstructured":"Margutti, P. et al. Autoantibodies to the C-terminal subunit of RLIP76 induce oxidative stress and endothelial cell apoptosis in immune-mediated vascular diseases and atherosclerosis. Blood 111, 4559\u20134570 (2008).","journal-title":"Blood"},{"key":"BFsrep08162_CR18","doi-asserted-by":"publisher","first-page":"R1133","DOI":"10.1186\/ar1798","volume":"7","author":"Y Lu","year":"2005","unstructured":"Lu, Y., Ye, P., Tan, E. M. & Chan, E. K. Identification of kinectin as a novel Beh\u00e7et's disease autoantigen. Arthritis Res. Ther. 7, R1133\u2013R1139 (2005).","journal-title":"Arthritis Res. Ther."},{"key":"BFsrep08162_CR19","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1016\/j.bbrc.2014.07.126","volume":"451","author":"Y Xun","year":"2014","unstructured":"Xun, Y. et al. Identification of prohibitin as an antigen in Behcet's disease. Biochem. Biophys. Res. Commun. 451, 389\u2013393 (2014).","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"BFsrep08162_CR20","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1038\/nrrheum.2012.66","volume":"8","author":"HT Maecker","year":"2012","unstructured":"Maecker, H. T. et al. New tools for classification and monitoring of autoimmune diseases. Nat. Rev. Rheumatol. 8, 317\u2013328 (2012).","journal-title":"Nat. Rev. Rheumatol."},{"key":"BFsrep08162_CR21","doi-asserted-by":"publisher","first-page":"6985","DOI":"10.1007\/s11033-014-3585-8","volume":"41","author":"H Du","year":"2014","unstructured":"Du, H. et al. Multiple correlations of mRNA expression and protein abundance in human cytokine profile. Mol. Biol. Rep. 41, 6985\u20136993 (2014).","journal-title":"Mol. Biol. Rep."},{"key":"BFsrep08162_CR22","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1039\/b708581h","volume":"3","author":"BA Macher","year":"2007","unstructured":"Macher, B. A. & Yen, T. Proteins at membrane surfaces-a review of approaches. Mol. Biosyst. 3, 705\u2013713 (2007).","journal-title":"Mol. Biosyst."},{"key":"BFsrep08162_CR23","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1186\/1471-2164-12-340","volume":"12","author":"C Backes","year":"2011","unstructured":"Backes, C. et al. Immunogenicity of autoantigens. BMC genomics 12, 340 (2011).","journal-title":"BMC genomics"},{"key":"BFsrep08162_CR24","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1186\/gb-2003-4-9-117","volume":"4","author":"D Greenbaum","year":"2003","unstructured":"Greenbaum, D., Colangelo, C., Williams, K. & Gerstein, M. Comparing protein abundance and mRNA expression levels on a genomic scale. Genome Biol. 4, 117 (2003).","journal-title":"Genome Biol."},{"key":"BFsrep08162_CR25","doi-asserted-by":"publisher","first-page":"3734","DOI":"10.1073\/pnas.80.12.3734","volume":"80","author":"CJ Edgell","year":"1983","unstructured":"Edgell, C. J., McDonald, C. C. & Graham, J. B. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc. Natl. Acad. Sci. USA 80, 3734\u20133737 (1983).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFsrep08162_CR26","doi-asserted-by":"publisher","first-page":"1174","DOI":"10.1189\/jlb.0307154","volume":"82","author":"JF Swisher","year":"2007","unstructured":"Swisher, J. F., Khatri, U. & Feldman, G. M. Annexin A2 is a soluble mediator of macrophage activation. J. Leukoc. Biol. 82, 1174\u20131184 (2007).","journal-title":"J. Leukoc. Biol."},{"key":"BFsrep08162_CR27","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1016\/j.autrev.2011.04.007","volume":"10","author":"L Iaccarino","year":"2011","unstructured":"Iaccarino, L. et al. Anti-annexins autoantibodies: Their role as biomarkers of autoimmune diseases. Autoimmun. Rev. 10, 553\u2013558 (2011).","journal-title":"Autoimmun. Rev."},{"key":"BFsrep08162_CR28","first-page":"1","volume":"2014","author":"C Wang","year":"2014","unstructured":"Wang, C. & Lin, C. Annexin A2: Its Molecular Regulation and Cellular Expression in Cancer Development. Dis. Markers 2014, 1\u201310 (2014).","journal-title":"Dis. Markers"},{"key":"BFsrep08162_CR29","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1007\/s12307-011-0064-9","volume":"4","author":"NA Lokman","year":"2011","unstructured":"Lokman, N. A., Ween, M. P., Oehler, M. K. & Ricciardelli, C. The role of annexin A2 in tumorigenesis and cancer progression. Cancer Microenviron. 4, 199\u2013208 (2011).","journal-title":"Cancer Microenviron."},{"key":"BFsrep08162_CR30","doi-asserted-by":"crossref","first-page":"21191","DOI":"10.1016\/S0021-9258(17)31947-6","volume":"269","author":"KA Hajjar","year":"1994","unstructured":"Hajjar, K. A., Jacovina, A. T. & Chacko, J. An endothelial cell receptor for plasminogen\/tissue plasminogen activator. I. Identity with annexin II. J. Biol. Chem. 269, 21191\u201321197 (1994).","journal-title":"J. Biol. Chem."},{"key":"BFsrep08162_CR31","doi-asserted-by":"publisher","first-page":"1964","DOI":"10.1182\/blood-2004-05-1708","volume":"105","author":"J Zhang","year":"2005","unstructured":"Zhang, J. & McCrae, K. R. Annexin A2 mediates endothelial cell activation by antiphospholipid\/anti-\u03b22 glycoprotein I antibodies. Blood 105, 1964\u20131969 (2005).","journal-title":"Blood"},{"key":"BFsrep08162_CR32","doi-asserted-by":"publisher","first-page":"865","DOI":"10.1007\/s00296-010-1379-4","volume":"31","author":"W Ao","year":"2011","unstructured":"Ao, W. et al. Anti-annexin II antibody is associated with thrombosis and\/or pregnancy morbidity in antiphospholipid syndrome and systemic lupus erythematosus with thrombosis. Rheumatol. Int. 31, 865\u2013869 (2011).","journal-title":"Rheumatol. Int."},{"key":"BFsrep08162_CR33","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1007\/s10875-008-9188-1","volume":"28","author":"V Salle","year":"2008","unstructured":"Salle, V. et al. Anti-annexin II antibodies in systemic autoimmune diseases and antiphospholipid syndrome. J. Clin. Immunol. 28, 291\u2013297 (2008).","journal-title":"J. Clin. Immunol."},{"key":"BFsrep08162_CR34","doi-asserted-by":"publisher","first-page":"4375","DOI":"10.1182\/blood-2005-07-2636","volume":"107","author":"G Cesarman-Maus","year":"2006","unstructured":"Cesarman-Maus, G. et al. Autoantibodies against the fibrinolytic receptor, annexin 2, in antiphospholipid syndrome. Blood 107, 4375\u20134382 (2006).","journal-title":"Blood"},{"key":"BFsrep08162_CR35","doi-asserted-by":"publisher","first-page":"1000","DOI":"10.1016\/j.molimm.2009.11.019","volume":"47","author":"Y Fang","year":"2010","unstructured":"Fang, Y. et al. Annexin A2 on lung epithelial cell surface is recognized by severe acute respiratory syndrome-associated coronavirus spike domain 2 antibodies. Mol. Immunol. 47, 1000\u20131009 (2010).","journal-title":"Mol. Immunol."},{"key":"BFsrep08162_CR36","doi-asserted-by":"publisher","first-page":"5775","DOI":"10.1128\/JVI.02343-09","volume":"84","author":"P Backes","year":"2010","unstructured":"Backes, P. et al. Role of annexin A2 in the production of infectious hepatitis C virus particles. J. Virol. 84, 5775\u20135789 (2010).","journal-title":"J. Virol."},{"key":"BFsrep08162_CR37","doi-asserted-by":"publisher","first-page":"1174","DOI":"10.1189\/jlb.0307154","volume":"82","author":"JF Swisher","year":"2007","unstructured":"Swisher, J. F., Khatri, U. & Feldman, G. M. Annexin A2 is a soluble mediator of macrophage activation. J. Leuko. Biol. 82, 1174\u20131184 (2007).","journal-title":"J. Leuko. Biol."},{"key":"BFsrep08162_CR38","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/S0923-1811(01)00086-X","volume":"26","author":"S Sohn","year":"2001","unstructured":"Sohn, S., Lee, E. & Lee, S. The correlation of MHC haplotype and development of Behcet's disease-like symptoms induced by herpes simplex virus in several inbred mouse strains. J. Dermatol. Sci. 26, 173\u2013181 (2001).","journal-title":"J. Dermatol. Sci."},{"key":"BFsrep08162_CR39","first-page":"506","volume":"16","author":"Y Mizushima","year":"1989","unstructured":"Mizushima, Y. Skin hypersensitivity to streptococcal antigens and the induction of systemic symptoms by the antigens in Beh\u00e7et's disease-A multicenter study. J. Rheumatol. 16, 506\u2013511 (1989).","journal-title":"J. Rheumatol."},{"key":"BFsrep08162_CR40","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/0003-9969(90)90113-O","volume":"35","author":"E Isogai","year":"1990","unstructured":"Isogai, E. et al. Chemiluminescence of neutrophils from patients with Beh\u00e7et's disease and its correlation with an increased proportion of uncommon serotypes of Streptococcus sanguis in the oral flora. Arch. Oral Biol. 35, 43\u201348 (1990).","journal-title":"Arch. Oral Biol."},{"key":"BFsrep08162_CR41","first-page":"489","volume":"18","author":"F Kaneko","year":"2008","unstructured":"Kaneko, F. et al. The role of streptococcal hypersensitivity in the pathogenesis of Beh\u00e7et's disease. Eur. J. Dermatol. 18, 489\u2013498 (2008).","journal-title":"Eur. J. Dermatol."},{"key":"BFsrep08162_CR42","first-page":"1","volume":"2","author":"E Isogai","year":"2002","unstructured":"Isogai, E. et al. Antibody cross reactivity from sera of patients with Behcet's disease with synthetic peptides that have homologies with protein from Streptococcus sanguis. J. Appl. Res. 2, 1\u20137 (2002).","journal-title":"J. Appl. Res."},{"key":"BFsrep08162_CR43","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.1128\/iai.59.4.1434-1441.1991","volume":"59","author":"T Lehner","year":"1991","unstructured":"Lehner, T. et al. Association between the 65-kilodalton heat shock protein, Streptococcus sanguis and the corresponding antibodies in Behcet's syndrome. Infect. Immun. 59, 1434\u20131441 (1991).","journal-title":"Infect. Immun."},{"key":"BFsrep08162_CR44","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1016\/S0140-6736(03)14075-5","volume":"362","author":"AG Pockley","year":"2003","unstructured":"Pockley, A. G. Heat shock proteins as regulators of the immune response. Lancet 362, 469\u2013476 (2003).","journal-title":"Lancet"},{"key":"BFsrep08162_CR45","doi-asserted-by":"publisher","first-page":"478","DOI":"10.1016\/S0140-6736(88)90123-7","volume":"2","author":"PC Res","year":"1988","unstructured":"Res, P. C. et al. Synovial fluid T cell reactivity against 65 kD heat shock protein of mycobacteria in early chronic arthritis. Lancet 2, 478\u2013480 (1988).","journal-title":"Lancet"},{"key":"BFsrep08162_CR46","doi-asserted-by":"publisher","first-page":"831","DOI":"10.1084\/jem.171.3.831","volume":"171","author":"JS Gaston","year":"1990","unstructured":"Gaston, J. S. et al. Recognition of a mycobacteria-specific epitope in the 65-kD heat-shock protein by synovial fluid-derived T cell clones. J. Exp. Med. 171, 831\u2013841 (1990).","journal-title":"J. Exp. Med."},{"key":"BFsrep08162_CR47","doi-asserted-by":"publisher","first-page":"1368","DOI":"10.1016\/0140-6736(91)93057-G","volume":"337","author":"ER De Graeff-Meeder","year":"1991","unstructured":"De Graeff-Meeder, E. R. et al. Recognition of human 60 kD heat shock protein by mononuclear cells from patients with juvenile chronic arthritis. Lancet 337, 1368\u20131372 (1991).","journal-title":"Lancet"},{"key":"BFsrep08162_CR48","doi-asserted-by":"publisher","first-page":"4588","DOI":"10.1073\/pnas.89.10.4588","volume":"89","author":"KW Wucherpfennig","year":"1992","unstructured":"Wucherpfennig, K. W. et al. Gamma delta T-cell receptor repertoire in acute multiple sclerosis lesions. Proc. Natl. Acad. Sci. USA 89, 4588\u20134592 (1992).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFsrep08162_CR49","doi-asserted-by":"crossref","first-page":"4883","DOI":"10.4049\/jimmunol.154.9.4883","volume":"15","author":"P Stinissen","year":"1995","unstructured":"Stinissen, P. Increased frequency of gamma delta T cells in cerebrospinal fluid and peripheral blood of patients with multiple sclerosis. Reactivity, cytotoxicity and T cell receptor V gene rearrangements. J. Immunol. 15, 4883\u20134894 (1995).","journal-title":"J. Immunol."},{"key":"BFsrep08162_CR50","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1007\/s00296-012-2446-9","volume":"33","author":"Aktas","year":"2013","unstructured":"Aktas, Cetin, E. et al. Beh\u00e7et's disease: immunological relevance with arthritis of ankylosing spondylitis. Rheumatol. Int. 33, 733\u2013741 (2013).","journal-title":"Rheumatol. Int."},{"key":"BFsrep08162_CR51","doi-asserted-by":"crossref","unstructured":"International Study Group for Beh\u00e7et's Disease. Criteria for diagnosis of Beh\u00e7et's disease. Lancet 335, 1078\u20131080 (1990).","DOI":"10.1016\/0140-6736(90)92643-V"},{"key":"BFsrep08162_CR52","doi-asserted-by":"publisher","first-page":"6166","DOI":"10.1021\/ac049159a","volume":"76","author":"H Du","year":"2004","unstructured":"Du, H. et al. Preparation of steroid antibodies and parallel detection of multianabolic steroid abuse with conjugated hapten microarray. Anal. Chem. 76, 6166\u20136171 (2004).","journal-title":"Anal. Chem."},{"key":"BFsrep08162_CR53","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1373\/clinchem.2004.036665","volume":"51","author":"H Du","year":"2005","unstructured":"Du, H. et al. Development of Miniaturized Competitive Immunoassays on a Protein Chip as a Screening Tool for Drugs. Clin. Chem. 51, 368\u2013375 (2005).","journal-title":"Clin. Chem."},{"key":"BFsrep08162_CR54","doi-asserted-by":"publisher","first-page":"2269","DOI":"10.4155\/bio.13.195","volume":"5","author":"H Du","year":"2013","unstructured":"Du, H., Li, C., Jin, H., Chen, G. & Xun, Y. Generation and evaluation of antibodies against human MGF E-peptide by reverse phase protein microarray and reverse competitive ELISA. Bioanalysis 5, 2269\u20132275 (2013).","journal-title":"Bioanalysis"},{"key":"BFsrep08162_CR55","doi-asserted-by":"publisher","first-page":"1862","DOI":"10.1002\/rcm.5055","volume":"25","author":"S Sun","year":"2011","unstructured":"Sun, S. et al. Efficient enrichment and identification of phosphopeptides by cerium oxide using on-plate matrix-assisted laser desorption\/ionization time-of-flight mass spectrometric analysis. Rapid Commun. Mass Spectrom. 25, 1862\u20131868 (2011).","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"BFsrep08162_CR56","doi-asserted-by":"publisher","first-page":"2161","DOI":"10.4155\/bio.12.188","volume":"4","author":"H Du","year":"2012","unstructured":"Du, H. et al. Immunological screening and characterization of highly specific monoclonal antibodies against 20 kDa hGH. Bioanalysis 4, 2161\u20132168 (2012).","journal-title":"Bioanalysis"},{"key":"BFsrep08162_CR57","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1038\/cmi.2012.57","volume":"10","author":"C Zhang","year":"2012","unstructured":"Zhang, C. et al. ATF4 is directly recruited by TLR4 signaling and positively regulates TLR4-trigged cytokine production in human monocytes. Cell Mol. Immunol. 10, 84\u201394 (2012).","journal-title":"Cell Mol. Immunol."},{"key":"BFsrep08162_CR58","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1186\/1479-5876-8-77","volume":"8","author":"C Sun","year":"2010","unstructured":"Sun, C. et al. Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke. J. Transl. Med. 8, 77 (2010).","journal-title":"J. Transl. Med."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep08162","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep08162.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep08162.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T00:43:32Z","timestamp":1672965812000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep08162"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,2]]},"references-count":58,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2015,7,15]]}},"alternative-id":["BFsrep08162"],"URL":"https:\/\/doi.org\/10.1038\/srep08162","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,2]]},"assertion":[{"value":"3 October 2014","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 January 2015","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 February 2015","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"8162"}}