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Specifically, we studied the influence of cation species and concentrations on DNA adsorption properties. Our results indicated that divalent cations (Mg<jats:sup>2+<\/jats:sup> and Co<jats:sup>2+<\/jats:sup>) preferred to form uniform and moderately dense DNA layers on a mica substrate. By measuring the interactions between DNA-coated mica and bare mica in an aqueous solution, obvious adhesion was observed in a cobalt chloride solution, possibly due to the ion-correlation attraction between negatively charged DNA and the mica surface. Furthermore, the interaction differences that were observed with MgCl<jats:sub>2<\/jats:sub> and CoCl<jats:sub>2<\/jats:sub> solutions reveal that the specific adsorption behaviors of DNA molecules on a mica substrate were mediated by these two salts. 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