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The AmB resistance mechanisms reported so far mainly involve decrease inergosterol content or alterations in cell wall. In contrast, depletion ofsphingolipids sensitizes cells to AmB. Recently, overexpression of <jats:italic>PMP3<\/jats:italic> gene,encoding plasma membrane proteolipid 3 protein, was shown to increase and itsdeletion to decrease, AmB resistance. Here we have explored the mechanistic basis of<jats:italic>PMP3<\/jats:italic> effect on AmB resistance. It was found that ergosterol content andcell wall integrity are not related to modulation of AmB resistance by <jats:italic>PMP3<\/jats:italic>.A few prominent phenotypes of <jats:italic>PMP3<\/jats:italic> delete strain, namely, defective actinpolarity, impaired salt tolerance and reduced rate of endocytosis are also notrelated to its AmB-sensitivity. However, <jats:italic>PMP3<\/jats:italic> overexpression mediatedincrease in AmB resistance requires a functional sphingolipid pathway. Moreover, AmBsensitivity of strains deleted in <jats:italic>PMP3<\/jats:italic> can be suppressed by the addition ofphytosphingosine, a sphingolipid pathway intermediate, confirming the importance ofthis pathway in modulation of AmB resistance by <jats:italic>PMP3<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/srep09685","type":"journal-article","created":{"date-parts":[[2015,5,12]],"date-time":"2015-05-12T15:20:43Z","timestamp":1431444043000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Plasma Membrane Proteolipid 3 Protein Modulates Amphotericin B Resistance throughSphingolipid Biosynthetic Pathway"],"prefix":"10.1038","volume":"5","author":[{"given":"Vinay K.","family":"Bari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sushma","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md.","family":"Alfatah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alok K.","family":"Mondal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Ganesan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,5,12]]},"reference":[{"key":"BFsrep09685_CR1","doi-asserted-by":"publisher","first-page":"165rv113","DOI":"10.1126\/scitranslmed.3004404","volume":"4","author":"GD Brown","year":"2012","unstructured":"Brown, G. 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