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We have used Phage Display to select scFv (single-chain variable fragment) clones from a combinatorial library against total proteins of<jats:italic>Strongyloides venezuelensis<\/jats:italic>. After scFv characterization, further analysis demonstrated that this recombinant fragment of antibody was able to bind to an<jats:italic>S. venezuelensis<\/jats:italic>antigenic fraction of ~65 kDa, present in the body periphery and digestive system of infective larvae (L3), as demonstrated by immunofluorescence. Mass spectrometry results followed by bioinformatics analysis showed that this antigenic fraction was a heat shock protein 60 (HSP60) of<jats:italic>Strongyloides<\/jats:italic>sp. The selected scFv was applied in serodiagnosis by immune complexes detection in serum samples from individuals with strongyloidiasis using a sandwich enzyme-linked immunosorbent assay (ELISA), showing sensitivity of 97.5% (86.84\u201399.94), specificity of 98.81 (93.54\u201399.97), positive likelihood ratio of 81.60 and an area under the curve of 0.9993 (0.9973\u20131.000). Our study provided a novel monoclonal scFv antibody fragment which specifically bound to HSP60 of<jats:italic>Strongyloides<\/jats:italic>sp. and was applied in the development of an innovative serodiagnosis method for the human strongyloidiasis.<\/jats:p>","DOI":"10.1038\/srep10447","type":"journal-article","created":{"date-parts":[[2015,5,21]],"date-time":"2015-05-21T11:09:19Z","timestamp":1432206559000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Structural and functional characterization of a novel scFv anti-HSP60 of Strongyloides sp."],"prefix":"10.1038","volume":"5","author":[{"given":"Marcelo Arantes","family":"Levenhagen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiana","family":"de Almeida Ara\u00fajo Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patr\u00edcia Tiemi","family":"Fujimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Paula","family":"Carneiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia Maria","family":"Costa-Cruz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Ricardo","family":"Goulart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,5,21]]},"reference":[{"key":"BFsrep10447_CR1","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1016\/j.trstmh.2009.02.013","volume":"103","author":"A Olsen","year":"2009","unstructured":"Olsen, A. et al. 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