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MPEG-PCL-CS block polymer was synthesized by covalent coupling of MPEG-PCL with CS. The critical micelle concentration of the MPEG-PCL-CS block polymer was 0.000692\u2009g\/L. DIC\/MPEG-PCL-CS nanosuspension (mean particle size\u2009=\u2009105\u2009nm, zeta potential\u2009=\u20098\u2009mV) was prepared and characterized by Fourier transform infrared spectroscopy, X-ray diffraction and differential scanning calorimetry. The nanosuspension was very stable without apparent physical property changes after storage at 4\u2009\u00b0C or 25\u2009\u00b0C for 20 days, but it was unstable in the aqueous humor solution after 24\u2009h incubation. Sustained release of the encapsulated DIC from the nanosuspension occurred over 8\u2009h. Neither a blank MPEG-PCL-CS nanosuspension nor a 0.1% (mass fraction) DIC\/MPEG-PCL-CS nanosuspension caused ocular irritation after 24\u2009h of instillation. Enhanced penetration and retention in corneal tissue was achieved with a Nile red\/MPEG-PCL-CS nanosuspension compared with a Nile red aqueous solution. <jats:italic>In vivo<\/jats:italic> pharmacokinetics studies showed enhanced pre-corneal retention and penetration of the DIC\/MPEG-PCL-CS nanosuspension, which resulted in a higher concentration of DIC (C<jats:sub>max<\/jats:sub>) in the aqueous humor and better bioavailability compared with commercial DIC eye drops (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01).<\/jats:p>","DOI":"10.1038\/srep11337","type":"journal-article","created":{"date-parts":[[2015,6,12]],"date-time":"2015-06-12T13:33:49Z","timestamp":1434116029000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":80,"title":["Chitosan grafted methoxy poly(ethylene glycol)-poly(\u03b5-caprolactone) nanosuspension for ocular delivery of hydrophobic diclofenac"],"prefix":"10.1038","volume":"5","author":[{"given":"Shuai","family":"Shi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoliang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zichao","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renlong","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,6,12]]},"reference":[{"key":"BFsrep11337_CR1","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/j.ejpb.2012.02.014","volume":"81","author":"C Di Tommaso","year":"2012","unstructured":"Di Tommaso, C. et al. 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