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However, little is known about the interaction between<jats:italic>C. parvum<\/jats:italic>and glycosaminoglycans. In this study, we assessed the inhibitory effect of sulfated polysaccharides on<jats:italic>C. parvum<\/jats:italic>invasion of host cells and identified the parasite ligands that interact with sulfated polysaccharides. Among five sulfated polysaccharides tested, heparin had the highest, dose-dependent inhibitory effect on parasite invasion. Heparan sulfate-deficient cells were less susceptible to<jats:italic>C. parvum<\/jats:italic>infection. We further identified 31 parasite proteins that potentially interact with heparin. Of these, we confirmed that<jats:italic>C. parvum<\/jats:italic>elongation factor 1\u03b1 (CpEF1\u03b1), which plays a role in<jats:italic>C. parvum<\/jats:italic>invasion, binds to heparin and to the surface of HCT-8 cells. 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