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The aggregation of A\u03b2 leads to diverse oligomeric states, each of which may be potential targets for intervention. Obtaining insight into A\u03b2 oligomers at the atomic level has been a major challenge to most techniques. Here, we use magic angle spinning recoupling\n                    <jats:sup>1<\/jats:sup>\n                    H-\n                    <jats:sup>1<\/jats:sup>\n                    H NMR experiments to overcome many of these limitations. Using\n                    <jats:sup>1<\/jats:sup>\n                    H-\n                    <jats:sup>1<\/jats:sup>\n                    H dipolar couplings as a NMR spectral filter to remove both high and low molecular weight species, we provide atomic-level characterization of a non-fibrillar aggregation product of the A\u03b2\n                    <jats:sub>1-40<\/jats:sub>\n                    peptide using non-frozen samples without isotopic labeling. Importantly, this spectral filter allows the detection of the specific oligomer signal without a separate purification procedure. In comparison to other solid-state NMR techniques, the experiment is extraordinarily selective and sensitive. A resolved 2D spectra could be acquired of a small population of oligomers (6 micrograms, 7% of the total) amongst a much larger population of monomers and fibers (93% of the total). By coupling real-time\n                    <jats:sup>1<\/jats:sup>\n                    H-\n                    <jats:sup>1<\/jats:sup>\n                    H NMR experiments with other biophysical measurements, we show that a stable, primarily disordered A\u03b2\n                    <jats:sub>1-40<\/jats:sub>\n                    oligomer 5\u201315\u2009nm in diameter can form and coexist in parallel with the well-known cross-\u03b2-sheet fibrils.\n                  <\/jats:p>","DOI":"10.1038\/srep11811","type":"journal-article","created":{"date-parts":[[2015,7,3]],"date-time":"2015-07-03T05:10:40Z","timestamp":1435900240000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":116,"title":["High-resolution NMR characterization of low abundance oligomers of amyloid-\u03b2 without purification"],"prefix":"10.1038","volume":"5","author":[{"given":"Samuel A.","family":"Kotler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey R.","family":"Brender","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subramanian","family":"Vivekanandan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuta","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazutoshi","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martine","family":"Monette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janarthanan","family":"Krishnamoorthy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Walsh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meagan","family":"Cauble","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark M. 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