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We report here that miR-125a-3p and miR-483-5p are upregulated in the SAT of MSL patients, promoting adipogenesis through suppressing the RhoA\/ROCK1\/ERK1\/2 pathway. TaqMan microRNA (miR) array analysis revealed that 18\u2009miRs were upregulated in the SAT of MSL patients. Transfection of human adipose-derived mesenchymal stem cells (hADSCs) with the individual agomirs of these 18\u2009miRs showed that miR-125a-3p and miR-483-5p significantly promoted adipogenesis. A dual-luciferase assay showed that RhoA and ERK1 were the targets of miR-125a-3p and miR-483-5p, respectively. Moreover, transfection of hADSCs with mimics of miR-125a-3p and miR-483-5p resulted in a pronounced decrease of ERK1\/2 phosphorylation in the nucleus; conversely, transfection of hADSCs with inhibitors of miR-125a-3p and miR-483-5p led to a significant increase of ERK1\/2 phosphorylation in the nucleus. Most importantly, we found that miR-125a-3p and miR-483-5p promoted<jats:italic>de novo<\/jats:italic>adipose tissue formation in nude mice. These results demonstrated that miR-125a-3p and miR-483-5p coordinately promoted adipogenesis through suppressing the RhoA\/ROCK1\/ERK1\/2 pathway. Our findings may provide novel strategies for the management and treatment of MSL or obesity.<\/jats:p>","DOI":"10.1038\/srep11909","type":"journal-article","created":{"date-parts":[[2015,7,7]],"date-time":"2015-07-07T10:08:15Z","timestamp":1436263695000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["miR-125a-3p and miR-483-5p promote adipogenesis via suppressing the RhoA\/ROCK1\/ERK1\/2 pathway in multiple symmetric lipomatosis"],"prefix":"10.1038","volume":"5","author":[{"given":"Ke","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honghui","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhong","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liling","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoli","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxing","family":"Wan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjun","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaohui","family":"Mo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,7,7]]},"reference":[{"key":"BFsrep11909_CR1","first-page":"211","volume":"38","author":"D Rendina","year":"2013","unstructured":"Rendina, D., De Filippo, G., Nazzaro, A. & Strazzullo, P. 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