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However, methicillin-susceptible <jats:italic>S. aureus<\/jats:italic> (MSSA) can also form biofilm <jats:italic>in vitro<\/jats:italic> and such biofilms are resistant to vancomycin. Hence, researching the possible mechanisms of MSSA biofilm formation is urgent and necessary. Here, we used <jats:italic>S. aureus<\/jats:italic> ATCC25923 as the model strain and studied gene expression profiles in biofilms after the treatment of ursolic acid and resveratrol using RNA-seq technology. The results showed that only ursolic acid could inhibit biofilm formation, which differed from their applied on the multiple clinical drugs resistant MRSA biofilm. RNA-seq data was validated by examining the expression of six genes involved in biofilm formation by qRT-PCR. These data analysis indicated that the mechanism of the MSSA biofilm formation was different from that of the MRSA, due to absence of accessory gene regulator (<jats:italic>agr<\/jats:italic>) function. These findings suggest that biofilms of <jats:italic>S. aureus<\/jats:italic> with <jats:italic>agr<\/jats:italic> dysfunction may be more resistant than those with <jats:italic>agr<\/jats:italic> function. Therefore, the infection from clinical MSSA may be recalcitrant once forming biofilm. Further study is necessary to uncover the mechanisms of biofilm formation in other clinical <jats:italic>S. aureus.<\/jats:italic><\/jats:p>","DOI":"10.1038\/srep11997","type":"journal-article","created":{"date-parts":[[2015,7,7]],"date-time":"2015-07-07T11:13:47Z","timestamp":1436267627000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Transcriptome analysis of the biofilm formed by methicillin-susceptible Staphylococcus aureus"],"prefix":"10.1038","volume":"5","author":[{"given":"Xiaojuan","family":"Tan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyang","family":"Sheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beiwen","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanshan","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhua","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiqun","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,7,7]]},"reference":[{"key":"BFsrep11997_CR1","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1099\/jmm.0.46804-0","volume":"56","author":"A Nostro","year":"2007","unstructured":"Nostro, A. et al. 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