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These anti-icing substances protect cells and tissues from freezing by inhibiting ice formation. In this study, we developed a method for coating an industrial metal material (aluminum, Al) with AFP from the Antarctic marine diatom, <jats:italic>Chaetoceros neogracile<\/jats:italic> (<jats:italic>Cn<\/jats:italic>-AFP), to prevent or delay ice formation. To coat Al with <jats:italic>Cn<\/jats:italic>-AFP, we used an Al-binding peptide (ABP) as a conjugator and fused it with <jats:italic>Cn<\/jats:italic>-AFP. The ABP bound well to the Al and did not considerably change the functional properties of AFP. <jats:italic>Cn<\/jats:italic>-AFP-coated Al (<jats:italic>Cn<\/jats:italic>-AFP-Al) showed a sufficiently low supercooling point. Additional trehalose coating of <jats:italic>Cn<\/jats:italic>-AFP-Al considerably delayed AFP denaturation on the Al without affecting its antifreeze activity. This metal surface\u2013coating method using trehalose-fortified AFP can be applied to other metals important in the aircraft and cold storage fields where anti-icing materials are critical.<\/jats:p>","DOI":"10.1038\/srep12019","type":"journal-article","created":{"date-parts":[[2015,7,8]],"date-time":"2015-07-08T11:08:50Z","timestamp":1436353730000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["Creating Anti-icing Surfaces via the Direct Immobilization of Antifreeze Proteins on Aluminum"],"prefix":"10.1038","volume":"5","author":[{"given":"Yunho","family":"Gwak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-in","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minjae","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong Suk","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myong Jong","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Jin","family":"Oh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young-Pil","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"EonSeon","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,7,8]]},"reference":[{"key":"BFsrep12019_CR1","doi-asserted-by":"crossref","unstructured":"Gent, R. 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