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Pneumococcal serotype 14 sequence type (ST) 46 had been the most prevalent clone causing pneumonia in children in Taiwan. A microarray was constructed using the genomic DNA of a clinical strain (NTUH-P15) of serotype 14 ST46. Using DNA hybridization, genomic variations in NTUH-P15 were compared to those of 3 control strains. Microarray analysis identified 7 genomic regions that had significant increases in hybridization signals in the NTUH-P15 strain compared to control strains. One of these regions encoded PblB, a phage-encoded virulence factor implicated (in\n                    <jats:italic>Streptococcus mitis<\/jats:italic>\n                    ) in infective endocarditis. The isogenic\n                    <jats:italic>pblB<\/jats:italic>\n                    mutant decreased adherence to A549 human lung epithelial cell compared to wild-type NTUH-P15 strain (P\u2009=\u20090.01). Complementation with\n                    <jats:italic>pblB<\/jats:italic>\n                    restored the adherence. PblB is predicted to contain a galactose-binding domain-like region. Preincubation of NTUH-P15 with D-galactose resulted in decreases of adherence to A549 cell in a dose-dependent manner. Challenge of mice with NTUH-P15, isogenic\n                    <jats:italic>pblB<\/jats:italic>\n                    mutant and\n                    <jats:italic>pblB<\/jats:italic>\n                    complementation strains determined that PblB was required for bacterial persistence in the nasopharynx and lung. PblB, as an adhesin mediating the galactose-specific adhesion activity of pneumococci, promote pneumococcal clonal success.\n                  <\/jats:p>","DOI":"10.1038\/srep12265","type":"journal-article","created":{"date-parts":[[2015,7,21]],"date-time":"2015-07-21T07:52:56Z","timestamp":1437465176000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Identification of PblB mediating galactose-specific adhesion in a successful Streptococcus pneumoniae clone"],"prefix":"10.1038","volume":"5","author":[{"given":"Yu-Chia","family":"Hsieh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu-Lung","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Che-Ming","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Town","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,7,21]]},"reference":[{"key":"BFsrep12265_CR1","doi-asserted-by":"publisher","first-page":"5513","DOI":"10.1016\/j.vaccine.2009.06.091","volume":"27","author":"YC Hsieh","year":"2009","unstructured":"Hsieh, Y. 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