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A rapid and accurate molecular detection assay is essential in populations where the deletions are common. In this study, gap-polymerase chain reaction (PCR) with high resolution melting (HRM) analysis was developed to detect both the large deletions. Melting curves at 86.9\u2009\u00b1\u20090.1\u2009\u00b0C were generated by normal individuals without the \u03b1-SEA deletion, 84.7\u2009\u00b1\u20090.1\u2009\u00b0C by homozygous \u03b1-SEA deletion individuals and two melting curves at 84.7\u2009\u00b1\u20090.1\u2009\u00b0C and 86.9\u2009\u00b1\u20090.1\u2009\u00b0C by \u03b1-SEA deletion carriers. Normal individuals without the \u03b2-FIL deletion produce amplicons with a melting temperature (Tm) at 74.6\u2009\u00b1\u20090.1\u2009\u00b0C, homozygous \u03b2-FIL individuals produce amplicons with Tm at 73.6\u2009\u00b1\u20090.1\u2009\u00b0C and heterozygous \u03b2-FIL individuals generate two amplicons with Tm at 73.6\u2009\u00b1\u20090.1\u2009\u00b0C and 74.6\u2009\u00b1\u20090.1\u2009\u00b0C. Evaluation using blinded tests on 220 DNA samples showed 100% sensitivity and specificity. The developed assays are sensitive and specific for rapid molecular and prenatal diagnosis for the \u03b1-SEA and \u03b2-FIL deletions.<\/jats:p>","DOI":"10.1038\/srep13937","type":"journal-article","created":{"date-parts":[[2015,9,14]],"date-time":"2015-09-14T09:08:36Z","timestamp":1442221716000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A novel gap-PCR with high resolution melting analysis for the detection of \u03b1-thalassaemia Southeast Asian and Filipino \u03b20-thalassaemia deletion"],"prefix":"10.1038","volume":"5","author":[{"given":"Siew","family":"Leng Kho","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kek","family":"Heng Chua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"George","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin Ai Mary Anne","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,9,14]]},"reference":[{"key":"BFsrep13937_CR1","doi-asserted-by":"crossref","unstructured":"Weatherall, D. 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