{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T12:39:48Z","timestamp":1793450388280,"version":"4.5.0"},"reference-count":46,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2015,9,9]],"date-time":"2015-09-09T00:00:00Z","timestamp":1441756800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2015,9,9]],"date-time":"2015-09-09T00:00:00Z","timestamp":1441756800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mass spectrometry (MS) in Selected Reaction Monitoring (SRM) mode is proposed for in-depth characterisation of microorganisms in a multiplexed analysis. Within 60\u201380\u2009minutes, the SRM method performs microbial identification (I), antibiotic-resistance detection (R), virulence assessment (V) and it provides epidemiological typing information (T). This SRM application is illustrated by the analysis of the human pathogen <jats:italic>Staphylococcus aureus,<\/jats:italic> demonstrating its promise for rapid characterisation of bacteria from positive blood cultures of sepsis patients.<\/jats:p>","DOI":"10.1038\/srep13944","type":"journal-article","created":{"date-parts":[[2015,9,9]],"date-time":"2015-09-09T09:55:51Z","timestamp":1441792551000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["Rapid Bacterial Identification, Resistance, Virulence and Type Profiling using Selected Reaction Monitoring Mass Spectrometry"],"prefix":"10.1038","volume":"5","author":[{"given":"Yannick","family":"Charretier","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Dauwalder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Franceschi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Degout-Charmette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Zambardi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiphaine","family":"Cecchini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chloe","family":"Bardet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Lacoux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Dufour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Veron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Rostaing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronique","family":"Lanet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanguy","family":"Fortin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Beaulieu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Perrot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Dechaume","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Pons","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Girard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Salvador","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9raldine","family":"Durand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Mallard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Theretz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Broyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Chatellier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaspard","family":"Gervasi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Van Nuenen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn","family":"Ann Roitsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Van Belkum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Lemoine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Vandenesch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Philippe","family":"Charrier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,9,9]]},"reference":[{"key":"BFsrep13944_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3109\/10408418109104485","volume":"9","author":"RF D\u2019Amato","year":"1981","unstructured":"D\u2019Amato, R. F., Holmes, B. & Bottone, E. J. The systems approach to diagnostic microbiology. Crit Rev. Microbiol. 9, 1\u201344 (1981).","journal-title":"Crit Rev. Microbiol."},{"key":"BFsrep13944_CR2","doi-asserted-by":"publisher","first-page":"4085","DOI":"10.1128\/JCM.00614-06","volume":"44","author":"MG Menozzi","year":"2006","unstructured":"Menozzi, M. G. et al. Two-center collaborative evaluation of performance of the BD phoenix automated microbiology system for identification and antimicrobial susceptibility testing of gram-negative bacteria. J. Clin. Microbiol. 44, 4085\u20134094 (2006).","journal-title":"J. Clin. Microbiol."},{"key":"BFsrep13944_CR3","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1021\/ac60352a007","volume":"47","author":"JP Anhalt","year":"1975","unstructured":"Anhalt, J. P. & Fenselau, C. Identification of bacteria using mass spectrometry. Anal. Chem. 47, 219\u2013225 (1975).","journal-title":"Anal. Chem."},{"key":"BFsrep13944_CR4","doi-asserted-by":"publisher","first-page":"3143","DOI":"10.1002\/pmic.201100049","volume":"11","author":"M Welker","year":"2011","unstructured":"Welker, M. Proteomics for routine identification of microorganisms. Proteomics. 11, 3143\u20133153 (2011).","journal-title":"Proteomics."},{"key":"BFsrep13944_CR5","doi-asserted-by":"publisher","first-page":"2018","DOI":"10.1128\/JCM.00313-13","volume":"51","author":"A Van Belkum","year":"2013","unstructured":"Van Belkum, A. & Dunne, W. M., Jr. Next-generation antimicrobial susceptibility testing. J. Clin. Microbiol. 51, 2018\u20132024 (2013).","journal-title":"J. Clin. Microbiol."},{"key":"BFsrep13944_CR6","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.5858\/arpa.2012-0651-OA","volume":"137","author":"KK Perez","year":"2012","unstructured":"Perez, K. K. et al. Integrating Rapid Pathogen Identification and Antimicrobial Stewardship Significantly Decreases Hospital Costs. Arch. Pathol. Lab Med. 137, 1247\u20131254 (2012).","journal-title":"Arch. Pathol. Lab Med."},{"key":"BFsrep13944_CR7","doi-asserted-by":"publisher","first-page":"e87870","DOI":"10.1371\/journal.pone.0087870","volume":"9","author":"A Machen","year":"2014","unstructured":"Machen, A., Drake, T. & Wang, Y. F. Same day identification and full panel antimicrobial susceptibility testing of bacteria from positive blood culture bottles made possible by a combined lysis-filtration method with MALDI-TOF VITEK mass spectrometry and the VITEK2 system. PLoS. One. 9, e87870 (2014).","journal-title":"PLoS. One."},{"key":"BFsrep13944_CR8","doi-asserted-by":"publisher","first-page":"949","DOI":"10.1007\/s10096-013-2031-5","volume":"33","author":"JS Jung","year":"2014","unstructured":"Jung, J. S. et al. Rapid detection of antibiotic resistance based on mass spectrometry and stable isotopes. Eur. J. Clin. Microbiol. Infect. Dis. 33, 949\u2013955 (2014).","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"BFsrep13944_CR9","doi-asserted-by":"publisher","first-page":"924","DOI":"10.1128\/JCM.02691-13","volume":"52","author":"JS Jung","year":"2014","unstructured":"Jung, J. S. et al. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid detection of beta-lactam resistance in Enterobacteriaceae derived from blood cultures. J. Clin. Microbiol. 52, 924\u2013930 (2014).","journal-title":"J. Clin. Microbiol."},{"key":"BFsrep13944_CR10","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1021\/pr200858r","volume":"11","author":"GP Hooff","year":"2012","unstructured":"Hooff, G. P. et al. Characterization of beta-lactamase enzyme activity in bacterial lysates using MALDI-mass spectrometry. J. Proteome. Res. 11, 79\u201384 (2012).","journal-title":"J. Proteome. Res."},{"key":"BFsrep13944_CR11","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1086\/421946","volume":"39","author":"H Wisplinghoff","year":"2004","unstructured":"Wisplinghoff, H. et al. Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study. Clin. Infect. Dis. 39, 309\u2013317 (2004).","journal-title":"Clin. Infect. Dis."},{"key":"BFsrep13944_CR12","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1016\/S1473-3099(02)00227-X","volume":"2","author":"DC Oliveira","year":"2002","unstructured":"Oliveira, D. C., Tomasz, A. & De Lencastre, H. Secrets of success of a human pathogen: molecular evolution of pandemic clones of meticillin-resistant Staphylococcus aureus. Lancet Infect. Dis. 2, 180\u2013189 (2002).","journal-title":"Lancet Infect. Dis."},{"key":"BFsrep13944_CR13","doi-asserted-by":"publisher","first-page":"4997","DOI":"10.1128\/AAC.01199-12","volume":"56","author":"T Ito","year":"2012","unstructured":"Ito, T. et al. Guidelines for reporting novel mecA gene homologues. Antimicrob. Agents Chemother. 56, 4997\u20134999 (2012).","journal-title":"Antimicrob. Agents Chemother."},{"key":"BFsrep13944_CR14","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.ijmm.2013.11.010","volume":"304","author":"JA Lindsay","year":"2014","unstructured":"Lindsay, J. A. Staphylococcus aureus genomics and the impact of horizontal gene transfer. Int. J. Med. Microbiol. 304, 103\u2013109 (2014).","journal-title":"Int. J. Med. Microbiol."},{"key":"BFsrep13944_CR15","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1016\/j.ijantimicag.2011.05.003","volume":"38","author":"Y Gillet","year":"2011","unstructured":"Gillet, Y. et al. Pragmatic management of Panton-Valentine leukocidin-associated staphylococcal diseases. Int. J. Antimicrob. Agents 38, 457\u2013464 (2011).","journal-title":"Int. J. Antimicrob. Agents"},{"key":"BFsrep13944_CR16","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/S1473-3099(09)70066-0","volume":"9","author":"E Lappin","year":"2009","unstructured":"Lappin, E. & Ferguson, A. J. Gram-positive toxic shock syndromes. Lancet Infect. Dis. 9, 281\u2013290 (2009).","journal-title":"Lancet Infect. Dis."},{"key":"BFsrep13944_CR17","doi-asserted-by":"publisher","first-page":"1128","DOI":"10.1086\/313461","volume":"29","author":"G Lina","year":"1999","unstructured":"Lina, G. et al. Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin. Infect. Dis. 29, 1128\u20131132 (1999).","journal-title":"Clin. Infect. Dis."},{"key":"BFsrep13944_CR18","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1016\/S0140-6736(02)07877-7","volume":"359","author":"Y Gillet","year":"2002","unstructured":"Gillet, Y. et al. Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patients. Lancet 359, 753\u2013759 (2002).","journal-title":"Lancet"},{"key":"BFsrep13944_CR19","doi-asserted-by":"publisher","first-page":"978","DOI":"10.3201\/eid0908.030089","volume":"9","author":"F Vandenesch","year":"2003","unstructured":"Vandenesch, F. et al. Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: worldwide emergence. Emerg. Infect. Dis. 9, 978\u2013984 (2003).","journal-title":"Emerg. Infect. Dis."},{"key":"BFsrep13944_CR20","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1038\/nmeth.2291","volume":"10","author":"P Picotti","year":"2013","unstructured":"Picotti, P., Bodenmiller, B. & Aebersold, R. Proteomics meets the scientific method. Nat. Methods 10, 24\u201327 (2013).","journal-title":"Nat. Methods"},{"key":"BFsrep13944_CR21","doi-asserted-by":"publisher","first-page":"1134","DOI":"10.1002\/pmic.201200042","volume":"12","author":"MS Bereman","year":"2012","unstructured":"Bereman, M. S., Maclean, B., Tomazela, D. M., Liebler, D. C. & Maccoss, M. J. The development of selected reaction monitoring methods for targeted proteomics via empirical refinement. Proteomics. 12, 1134\u20131141 (2012).","journal-title":"Proteomics."},{"key":"BFsrep13944_CR22","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1016\/j.ymeth.2004.08.018","volume":"35","author":"DS Kirkpatrick","year":"2005","unstructured":"Kirkpatrick, D. S., Gerber, S. A. & Gygi, S. P. The absolute quantification strategy: a general procedure for the quantification of proteins and post-translational modifications. Methods 35, 265\u2013273 (2005).","journal-title":"Methods"},{"key":"BFsrep13944_CR23","doi-asserted-by":"publisher","first-page":"534","DOI":"10.1111\/j.1469-0691.2008.01986.x","volume":"14","author":"S Monecke","year":"2008","unstructured":"Monecke, S., Jatzwauk, L., Weber, S., Slickers, P. & Ehricht, R. DNA microarray-based genotyping of methicillin-resistant Staphylococcus aureus strains from Eastern Saxony. Clin. Microbiol. Infect. 14, 534\u2013545 (2008).","journal-title":"Clin. Microbiol. Infect."},{"key":"BFsrep13944_CR24","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1007\/978-1-60327-999-4_15","volume":"551","author":"M Hallin","year":"2009","unstructured":"Hallin, M., Friedrich, A. W. & Struelens, M. J. spa typing for epidemiological surveillance of Staphylococcus aureus. Methods Mol. Biol. 551, 189\u2013202 (2009).","journal-title":"Methods Mol. Biol."},{"key":"BFsrep13944_CR25","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.5858\/arpa.2012-0651-OA","volume":"137","author":"KK Perez","year":"2013","unstructured":"Perez, K. K. et al. Integrating rapid pathogen identification and antimicrobial stewardship significantly decreases hospital costs. Arch. Pathol. Lab Med. 137, 1247\u20131254 (2013).","journal-title":"Arch. Pathol. Lab Med."},{"key":"BFsrep13944_CR26","doi-asserted-by":"publisher","first-page":"2452","DOI":"10.1128\/JCM.00409-12","volume":"50","author":"JL Wimmer","year":"2012","unstructured":"Wimmer, J. L. et al. Strategy for rapid identification and antibiotic susceptibility testing of gram-negative bacteria directly recovered from positive blood cultures using the Bruker MALDI Biotyper and the BD Phoenix system. J. Clin. Microbiol. 50, 2452\u20132454 (2012).","journal-title":"J. Clin. Microbiol."},{"key":"BFsrep13944_CR27","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1099\/jmm.0.035550-0","volume":"61","author":"S Klein","year":"2012","unstructured":"Klein, S. et al. Integration of matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry in blood culture diagnostics: a fast and effective approach. J. Med. Microbiol. 61, 323\u2013331 (2012).","journal-title":"J. Med. Microbiol."},{"key":"BFsrep13944_CR28","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1021\/pr034086h","volume":"3","author":"NL Anderson","year":"2004","unstructured":"Anderson, N. L. et al. Mass spectrometric quantitation of peptides and proteins using Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA). J. Proteome. Res. 3, 235\u2013244 (2004).","journal-title":"J. Proteome. Res."},{"key":"BFsrep13944_CR29","doi-asserted-by":"publisher","first-page":"6940","DOI":"10.1073\/pnas.0832254100","volume":"100","author":"SA Gerber","year":"2003","unstructured":"Gerber, S. A., Rush, J., Stemman, O., Kirschner, M. W. & Gygi, S. P. Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS. Proc. Natl. Acad. Sci. USA 100, 6940\u20136945 (2003).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFsrep13944_CR30","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1038\/nmeth774","volume":"2","author":"RJ Beynon","year":"2005","unstructured":"Beynon, R. J., Doherty, M. K., Pratt, J. M. & Gaskell, S. J. Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides. Nat. Methods 2, 587\u2013589 (2005).","journal-title":"Nat. Methods"},{"key":"BFsrep13944_CR31","doi-asserted-by":"publisher","first-page":"4633","DOI":"10.1002\/pmic.200800326","volume":"8","author":"A Dupuis","year":"2008","unstructured":"Dupuis, A., Hennekinne, J. A., Garin, J. & Brun, V. Protein Standard Absolute Quantification (PSAQ) for improved investigation of staphylococcal food poisoning outbreaks. Proteomics. 8, 4633\u20134636 (2008).","journal-title":"Proteomics."},{"key":"BFsrep13944_CR32","doi-asserted-by":"publisher","first-page":"2623","DOI":"10.1074\/mcp.M112.027078","volume":"12","author":"SE Abbatiello","year":"2013","unstructured":"Abbatiello, S. E. et al. Design, implementation and multisite evaluation of a system suitability protocol for the quantitative assessment of instrument performance in liquid chromatography-multiple reaction monitoring-MS (LC-MRM-MS). Mol. Cell Proteomics. 12, 2623\u20132639 (2013).","journal-title":"Mol. Cell Proteomics."},{"key":"BFsrep13944_CR33","doi-asserted-by":"publisher","first-page":"1602","DOI":"10.1038\/nprot.2013.091","volume":"8","author":"S Surinova","year":"2013","unstructured":"Surinova, S. et al. Automated selected reaction monitoring data analysis workflow for large-scale targeted proteomic studies. Nat. Protoc. 8, 1602\u20131619 (2013).","journal-title":"Nat. Protoc."},{"key":"BFsrep13944_CR34","doi-asserted-by":"publisher","first-page":"2513","DOI":"10.1074\/mcp.M113.031591","volume":"13","author":"J Cox","year":"2014","unstructured":"Cox, J. et al. Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ. Mol. Cell Proteomics. 13, 2513\u20132526 (2014).","journal-title":"Mol. Cell Proteomics."},{"key":"BFsrep13944_CR35","doi-asserted-by":"publisher","first-page":"1041","DOI":"10.1038\/nmeth.3072","volume":"11","author":"JD Worboys","year":"2014","unstructured":"Worboys, J. D., Sinclair, J., Yuan, Y. & Jorgensen, C. Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome. Nat. Methods 11, 1041\u20131044 (2014).","journal-title":"Nat. Methods"},{"key":"BFsrep13944_CR36","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1021\/pr5010955","volume":"14","author":"Y Mohammed","year":"2015","unstructured":"Mohammed, Y., Percy, A. J., Chambers, A. G. & Borchers, C. H. Qualis-SIS: Automated Standard Curve Generation and Quality Assessment for Multiplexed Targeted Quantitative Proteomic Experiments with Labeled Standards. J. Proteome. Res. 14, 1137\u20131146 (2015).","journal-title":"J. Proteome. Res."},{"key":"BFsrep13944_CR37","doi-asserted-by":"publisher","first-page":"81","DOI":"10.3389\/fmicb.2015.00081","volume":"6","author":"Y Charretier","year":"2015","unstructured":"Charretier, Y. et al. Label-free SRM-based relative quantification of antibiotic resistance mechanisms in Pseudomonas aeruginosa clinical isolates. Front Microbiol. 6, 81 (2015).","journal-title":"Front Microbiol."},{"key":"BFsrep13944_CR38","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1586\/erm.12.32","volume":"12","author":"J Lemoine","year":"2012","unstructured":"Lemoine, J. et al. The current status of clinical proteomics and the use of MRM and MRM(3) for biomarker validation. Expert. Rev. Mol. Diagn. 12, 333\u2013342 (2012).","journal-title":"Expert. Rev. Mol. Diagn."},{"key":"BFsrep13944_CR39","doi-asserted-by":"publisher","first-page":"1006","DOI":"10.1074\/mcp.M800238-MCP200","volume":"8","author":"T Fortin","year":"2009","unstructured":"Fortin, T. et al. Clinical quantitation of prostate-specific antigen biomarker in the low nanogram\/milliliter range by conventional bore liquid chromatography-tandem mass spectrometry (multiple reaction monitoring) coupling and correlation with ELISA tests. Mol. Cell Proteomics. 8, 1006\u20131015 (2009).","journal-title":"Mol. Cell Proteomics."},{"key":"BFsrep13944_CR40","doi-asserted-by":"publisher","first-page":"1589","DOI":"10.1097\/01.CCM.0000217961.75225.E9","volume":"34","author":"A Kumar","year":"2006","unstructured":"Kumar, A. et al. Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med. 34, 1589\u20131596 (2006).","journal-title":"Crit Care Med."},{"key":"BFsrep13944_CR41","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1128\/JCM.41.1.254-260.2003","volume":"41","author":"P Francois","year":"2003","unstructured":"Francois, P. et al. Rapid detection of methicillin-resistant Staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assay. J. Clin. Microbiol. 41, 254\u2013260 (2003).","journal-title":"J. Clin. Microbiol."},{"key":"BFsrep13944_CR42","doi-asserted-by":"publisher","first-page":"3309","DOI":"10.1128\/JCM.42.7.3309-3312.2004","volume":"42","author":"P Francois","year":"2004","unstructured":"Francois, P. et al. A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements. J. Clin. Microbiol. 42, 3309\u20133312 (2004).","journal-title":"J. Clin. Microbiol."},{"key":"BFsrep13944_CR43","doi-asserted-by":"publisher","first-page":"304","DOI":"10.3201\/eid1402.070229","volume":"14","author":"P Francois","year":"2008","unstructured":"Francois, P. et al. Methicillin-resistant Staphylococcus aureus, Geneva, Switzerland, 1993-2005. Emerg. Infect. Dis. 14, 304\u2013307 (2008).","journal-title":"Emerg. Infect. Dis."},{"key":"BFsrep13944_CR44","doi-asserted-by":"publisher","first-page":"5442","DOI":"10.1128\/JCM.41.12.5442-5448.2003","volume":"41","author":"D Harmsen","year":"2003","unstructured":"Harmsen, D. et al. Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J. Clin. Microbiol. 41, 5442\u20135448 (2003).","journal-title":"J. Clin. Microbiol."},{"key":"BFsrep13944_CR45","doi-asserted-by":"publisher","first-page":"1159","DOI":"10.1007\/s10096-009-0752-2","volume":"28","author":"S Monecke","year":"2009","unstructured":"Monecke, S., Luedicke, C., Slickers, P. & Ehricht, R. Molecular epidemiology of Staphylococcus aureus in asymptomatic carriers. Eur. J. Clin. Microbiol. Infect. Dis. 28, 1159\u20131165 (2009).","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"BFsrep13944_CR46","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1128\/JCM.38.3.1008-1015.2000","volume":"38","author":"MC Enright","year":"2000","unstructured":"Enright, M. C., Day, N. P., Davies, C. E., Peacock, S. J. & Spratt, B. G. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J. Clin. Microbiol. 38, 1008\u20131015 (2000).","journal-title":"J. Clin. Microbiol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep13944","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep13944.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep13944.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T00:09:39Z","timestamp":1672963779000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep13944"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,9]]},"references-count":46,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2015,11,26]]}},"alternative-id":["BFsrep13944"],"URL":"https:\/\/doi.org\/10.1038\/srep13944","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,9]]},"assertion":[{"value":"29 September 2014","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 August 2015","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 September 2015","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Y.C., C.F., E.D.C., G.Z., T.C., C.Ba., X.L., P.D., L.V., H.R., V.L., T.F., C.Be., N.P., D.D., S.P., V.G., G.D., F.M., A.T., P.B., S.C., G.G., M.V.N., C. R., A.V.B. and J.P.C. are, or were, employed by bioM\u00e9rieux. Y.C., C.F., P.D., C.Be., S.P., V.G., S.C. and J.P.C. have filed a patent assigned to bioM\u00e9rieux. O. D., A.S., J.L. and F.V. report research grants from bioM\u00e9rieux.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"13944"}}