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However, the pathway through which <jats:italic>Mycobacterium tuberculosis<\/jats:italic> responds INH remain largely unclear. In this study, we characterized a novel transcriptional factor, InbR, which is encoded by Rv0275c and belongs to the TetR family, that is directly responsive to INH. Disrupting <jats:italic>inbR<\/jats:italic> made mycobacteria more sensitive to INH, whereas overexpressing <jats:italic>inbR<\/jats:italic> decreased bacterial susceptibility to the drug. InbR could bind specifically to the upstream region of its own operon at two inverted repeats and act as an auto-repressor. Furthermore, InbR directly bind with INH and the binding reduced InbR\u2019s DNA-binding ability. Interestingly, susceptibilities were also changed by InbR for other anti-TB drugs, such as rifampin, implying that InbR may play a role in multi-drug resistance. Additionally, microarray analyses revealed a portion genes of the <jats:italic>inbR<\/jats:italic> regulon have similar expression patterns in <jats:italic>inbR<\/jats:italic>-overexpressing strain and INH-treated wild type strain, suggesting that these genes, for example <jats:italic>iniBAC<\/jats:italic>, may be responsible to the drug resistance of <jats:italic>inbR<\/jats:italic>-overexpressing strain. The regulation of these genes by InbR were further assessed by ChIP-seq assay. InbR may regulate multiple drug resistance of mycobacteria through the regulation of these genes.<\/jats:p>","DOI":"10.1038\/srep13969","type":"journal-article","created":{"date-parts":[[2015,9,10]],"date-time":"2015-09-10T09:15:58Z","timestamp":1441876558000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["InbR, a TetR family regulator, binds with isoniazid and influences multidrug resistance in Mycobacterium bovis BCG"],"prefix":"10.1038","volume":"5","author":[{"given":"Min","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Hui","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialing","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoyun","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng-Guo","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,9,10]]},"reference":[{"key":"BFsrep13969_CR1","doi-asserted-by":"publisher","first-page":"73","DOI":"10.2174\/187152607781001844","volume":"7","author":"RC Goldman","year":"2007","unstructured":"Goldman, R. 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