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There is no specific antiviral for treatment of patients infected with CHIKV and development of compounds with significant anti-CHIKV activity that can be further developed to a practical therapy is urgently required. Andrographolide is derived from <jats:italic>Andrographis paniculata<\/jats:italic>, a herb traditionally used to treat a number of conditions including infections. This study sought to determine the potential of andrographolide as an inhibitor of CHIKV infection. Andrographolide showed good inhibition of CHIKV infection and reduced virus production by approximately 3log<jats:sub>10<\/jats:sub> with a 50% effective concentration (EC<jats:sub>50<\/jats:sub>) of 77\u2009\u03bcM without cytotoxicity. Time-of-addition and RNA transfection studies showed that andrographolide affected CHIKV replication and the activity of andrographolide was shown to be cell type independent. 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