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Transcription analyses were conducted to verify the roles of RDase subunit A genes (<jats:italic>rdhA<\/jats:italic>) in chloroethene respiration. Some interesting features of the strain MB draft genome include a large genome size, two CRISPR-<jats:italic>cas<\/jats:italic> type I systems and 38 <jats:italic>rdhA<\/jats:italic> genes. Strain 11a has a stream-lined genome with 11 <jats:italic>rdhA<\/jats:italic> genes, of which nine are distinct. Quantitative real-time PCR transcription analysis of RDase gene transcripts showed that a single RDase gene, designated <jats:italic>mbrA<\/jats:italic>, was up-regulated upon exposure to TCE and no other RDase genes were considerably expressed in strain MB. A single RDase gene, designated <jats:italic>vcrA<\/jats:italic>, was up-regulated upon exposure to TCE and expressed at a steady level until all chloroethenes were completely dechlorinated to ethene at 147\u2009h in strain 11a. Overall, this study reports the genomes of two distinct <jats:italic>Dhc<\/jats:italic> strains; both contain numerous uncharacterized RDase genes, but in each strain only one such gene was expressed highly during organohalide respiration.<\/jats:p>","DOI":"10.1038\/srep15204","type":"journal-article","created":{"date-parts":[[2015,11,6]],"date-time":"2015-11-06T09:47:25Z","timestamp":1446803245000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["A comparative genomics and reductive dehalogenase gene transcription study of two chloroethene-respiring bacteria, Dehalococcoides mccartyi strains MB and 11a"],"prefix":"10.1038","volume":"5","author":[{"given":"Adrian","family":"Low","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Rogers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick K. 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