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In this study, it is demonstrated that \u03b2-BA ameliorates plasma coagulation parameters, protects endothelium from blood stasis induced injury and prevents blood stasis induced impairment of endothelium-dependent vasodilatation. Moreover, it is found that \u03b2-BA significantly increases nitric oxide (NO) and cyclic guanosine 3\u2019, 5\u2019-monophosphate (cGMP) levels in carotid aortas of blood stasis rats. To stimulate blood stasis-like conditions<jats:italic>in vitro<\/jats:italic>, human umbilical vein endothelial cells (HUVECs) were exposed to transient oxygen and glucose deprivation (OGD). Treatment of \u03b2-BA significantly increased intracellular NO level. Western blot and immunofluorescence as well as immunohistochemistry reveal that \u03b2-BA increases phosphorylation of enzyme nitric oxide synthase (eNOS) at Ser1177. In addition, \u03b2-BA mediated endothelium-dependent vasodilatation can be markedly blocked by eNOS inhibitor L-NAME in blood stasis rats. In OGD treated HUEVCs, the protective effect of \u03b2-BA is attenuated by knockdown of eNOS. In conclusion, the above findings provide convincing evidence for the protective effects of \u03b2-BA on blood stasis induced endothelial dysfunction by eNOS signaling pathway.<\/jats:p>","DOI":"10.1038\/srep15357","type":"journal-article","created":{"date-parts":[[2015,10,20]],"date-time":"2015-10-20T09:32:18Z","timestamp":1445333538000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Pretreatment with \u03b2-Boswellic Acid Improves Blood Stasis Induced Endothelial Dysfunction: Role of eNOS Activation"],"prefix":"10.1038","volume":"5","author":[{"given":"Mingming","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minchun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Ding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihui","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yikai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peifeng","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingwen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Qiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuwen","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aidong","family":"Wen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,10,20]]},"reference":[{"key":"BFsrep15357_CR1","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/j.cbi.2010.10.017","volume":"189","author":"G Chashoo","year":"2011","unstructured":"Chashoo, G. et al. 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