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Batf3-dependent dendritic cells specialize in cross-presentation of necrotic tissue-derived epitopes to directly activate cytolytic CD8 Tcells. The mature plaque (necrotic, containing dendritic cells and CD8 Tcells) could offer the ideal environment for cross-presentation, resulting in cytotoxic immunity and plaque destabilization. <jats:italic>Ldlr<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice were transplanted with <jats:italic>batf3<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> or wt bone marrow and put on a western type diet. Hematopoietic batf3 deficiency sharply decreased CD8\u03b1<jats:sup>+<\/jats:sup> DC numbers in spleen and lymph nodes (&gt;80%; P\u2009&lt;\u20090,001). Concordantly, <jats:italic>batf3<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> chimeras had a 75% reduction in OT-I cross-priming capacity <jats:italic>in vivo<\/jats:italic>. <jats:italic>Batf3<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> chimeric mice did not show lower Tcell or other leukocyte subset numbers. Despite dampened cross-presentation capacity, <jats:italic>batf3<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> chimeras had equal atherosclerosis burden in aortic arch and root. Likewise, <jats:italic>batf3<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> chimeras and wt mice revealed no differences in parameters of plaque stability: plaque Tcell infiltration, cell death, collagen composition and macrophage and vascular smooth muscle cell content were unchanged. These results show that CD8\u03b1<jats:sup>+<\/jats:sup> DC loss in hyperlipidemic mice profoundly reduces cross-priming ability, nevertheless it does not influence lesion development. Taken together, we clearly demonstrate that CD8\u03b1<jats:sup>+<\/jats:sup> DC-mediated cross-presentation does not significantly contribute to atherosclerotic plaque formation and stability.<\/jats:p>","DOI":"10.1038\/srep15414","type":"journal-article","created":{"date-parts":[[2015,10,21]],"date-time":"2015-10-21T09:04:47Z","timestamp":1445418287000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Ablation of CD8\u03b1+ dendritic cell mediated cross-presentation does not impact atherosclerosis in hyperlipidemic mice"],"prefix":"10.1038","volume":"5","author":[{"given":"Bart","family":"Legein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edith M.","family":"Janssen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas L.","family":"Theelen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion J.","family":"Gijbels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joep","family":"Walraven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jared S.","family":"Klarquist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassandra M.","family":"Hennies","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristiaan","family":"Wouters","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom T.P.","family":"Seijkens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erwin","family":"Wijnands","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith C.","family":"Sluimer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Lutgens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Zenke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Hildner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik A.L.","family":"Biessen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lieve","family":"Temmerman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,10,21]]},"reference":[{"key":"BFsrep15414_CR1","doi-asserted-by":"publisher","first-page":"508","DOI":"10.1038\/nri1882","volume":"6","author":"GK Hansson","year":"2006","unstructured":"Hansson, G. 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