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We sought to establish and evaluate a reporter gene system using <jats:italic>TYR<\/jats:italic> under the control of the Tet-on gene expression system (gene expression induced by doxycycline [Dox]) as a multimodality imaging agent. We transfected <jats:italic>TYR<\/jats:italic> into human breast cancer cells (MDA-MB-231), naming the resulting cell line 231-TYR. Using non-transfected MDA-MB-231 cells as a control, we verified successful expression of <jats:italic>TYR<\/jats:italic> by 231-TYR after incubation with Dox using western blot, cellular tyrosinase activity, Masson-Fontana silver staining and a cell immunofluorescence study, while the control cells and 231-TYR cells without Dox exposure revealed no <jats:italic>TYR<\/jats:italic> expression. Detected by its absorbance at 405\u2009nm, increasing concentrations of melanin correlated positively with Dox concentration and incubation time. <jats:italic>TYR<\/jats:italic> expression by Dox-induced transfected cells shortened MRI T1 and T2 relaxation times. Photoacoustic signals were easily detected in these cells. <jats:sup>18<\/jats:sup>F-5-fluoro-N-(2-[diethylamino]ethyl)picolinamide (<jats:sup>18<\/jats:sup>F-5-FPN), which targets melanin, quickly accumulated in Dox-induced 231-TYR cells. These show that <jats:italic>TYR<\/jats:italic> induction of melanin production is regulated by the Tet-on system and TYR-containing indicator cells may have utility in multimodality imaging.<\/jats:p>","DOI":"10.1038\/srep15502","type":"journal-article","created":{"date-parts":[[2015,10,20]],"date-time":"2015-10-20T09:43:16Z","timestamp":1445334196000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["TYR as a multifunctional reporter gene regulated by the Tet-on system for multimodality imaging: an in vitro study"],"prefix":"10.1038","volume":"5","author":[{"given":"Hongyan","family":"Feng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaotian","family":"Xia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chongjiao","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiling","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunxia","family":"Qin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongxue","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Lan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,10,20]]},"reference":[{"key":"BFsrep15502_CR1","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1101\/gad.1047403","volume":"17","author":"TF Massoud","year":"2003","unstructured":"Massoud, T. 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