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Therefore, we examined the suitability of three anesthetic regimes for sufficient sedation of the chick embryo. Medetomidine at a dosage of 0.3 mg\/kg, was compared to thiopental at 100\u2009mg\/kg and ketamine\/midazolam at 50\u2009mg\/kg and 1\u2009mg\/kg, respectively. These soluble anesthetics were applied by dropping a total volume of 0.3\u2009mL onto the surface of the CAM. Motion was videotaped through the window of the eggshell and scored semi-quantitatively. Medetomidine performed best in terms of reduced motion; onset of anesthesia occurred within 10\u2009minutes and for the following 30\u2009minutes, allowing proper <jats:italic>in vivo<\/jats:italic> MRI measurements. The other regimen were not sedating deep enough (ketamine\/midazolam) and not long enough (thiopental). In sum, medetomidine allows proper sedation for MRI assessment of the perfusion capacity in a tissue engineered construct placed on the CAM.<\/jats:p>","DOI":"10.1038\/srep15536","type":"journal-article","created":{"date-parts":[[2015,10,23]],"date-time":"2015-10-23T09:32:03Z","timestamp":1445592723000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Comparison of medetomidine, thiopental and ketamine\/midazolam anesthesia in chick embryos for in ovo Magnetic Resonance Imaging free of motion artifacts"],"prefix":"10.1038","volume":"5","author":[{"given":"Conny","family":"Waschkies","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flora","family":"Nicholls","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Buschmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,10,23]]},"reference":[{"key":"BFsrep15536_CR1","doi-asserted-by":"publisher","first-page":"1244","DOI":"10.1016\/j.mri.2011.07.022","volume":"29","author":"IE Chesnick","year":"2011","unstructured":"Chesnick, I. 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