{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:38:38Z","timestamp":1790818718992,"version":"4.1.0"},"reference-count":60,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2015,10,23]],"date-time":"2015-10-23T00:00:00Z","timestamp":1445558400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2015,10,23]],"date-time":"2015-10-23T00:00:00Z","timestamp":1445558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The Lin28\/let-7 system, which includes the RNA-binding proteins, Lin28a\/Lin28b and let-7 miRNAs, has emerged as putative regulator of puberty and male gametogenesis; yet, its expression pattern and regulation in postnatal testis remain ill defined. We report herein expression profiles of Lin28 and let-7 members and related mir-145 and mir-132, in rat testis during postnatal maturation and in models of altered puberty and hormonal deregulation. Neonatal expression of Lin28a and Lin28b was low and rose markedly during the infantile period; yet, expression patterns diverged thereafter, with persistently elevated levels only for Lin28b, which peaked at puberty. Let-7a, let-7b, mir-132 and mir-145 showed profiles opposite to Lin28b. In fact, let-7b and mir-145 were abundant in pachytene spermatocytes, but absent in elongating spermatids, where high expression of Lin28b was previously reported. Perturbation of puberty by neonatal estrogenization reverted the Lin28\/let-7 expression ratio; expression changes were also detected in other models of delayed puberty, due to early photoperiod or nutritional manipulations. In addition, hypophysectomy or growth hormone (GH) deficiency revealed regulation of this system by gonadotropins and GH. Our data document the expression profiles of the Lin28\/let-7 system in rat testis along postnatal\/pubertal maturation and their perturbation in models of pubertal and hormonal manipulation.<\/jats:p>","DOI":"10.1038\/srep15683","type":"journal-article","created":{"date-parts":[[2015,10,23]],"date-time":"2015-10-23T06:14:08Z","timestamp":1445580848000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Testicular expression of the Lin28\/let-7 system: Hormonal regulation and changes during postnatal maturation and after manipulations of puberty"],"prefix":"10.1038","volume":"5","author":[{"given":"S.","family":"Sangiao-Alvarellos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Manfredi-Lozano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Ruiz-Pino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Le\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Morales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Cordido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Gayt\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Pinilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Tena-Sempere","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,10,23]]},"reference":[{"key":"BFsrep15683_CR1","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1126\/science.6494891","volume":"226","author":"V Ambros","year":"1984","unstructured":"Ambros, V. & Horvitz, H. R. Heterochronic mutants of the nematode Caenorhabditis elegans. Science 226, 409\u2013416 (1984).","journal-title":"Science"},{"key":"BFsrep15683_CR2","doi-asserted-by":"publisher","first-page":"432","DOI":"10.1016\/S0012-1606(03)00126-X","volume":"258","author":"EG Moss","year":"2003","unstructured":"Moss, E. G. & Tang, L. Conservation of the heterochronic regulator Lin-28, its developmental expression and microRNA complementary sites. Dev Biol 258, 432\u2013442 (2003).","journal-title":"Dev Biol"},{"key":"BFsrep15683_CR3","doi-asserted-by":"publisher","first-page":"16","DOI":"10.4161\/rna.4.1.4364","volume":"4","author":"E Balzer","year":"2007","unstructured":"Balzer, E. & Moss, E. G. Localization of the developmental timing regulator Lin28 to mRNP complexes, P-bodies and stress granules. RNA Biol 4, 16\u201325 (2007).","journal-title":"RNA Biol"},{"key":"BFsrep15683_CR4","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.gene.2006.07.011","volume":"384","author":"Y Guo","year":"2006","unstructured":"Guo, Y. et al. Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma. Gene 384, 51\u201361 (2006).","journal-title":"Gene"},{"key":"BFsrep15683_CR5","doi-asserted-by":"publisher","first-page":"372","DOI":"10.1046\/j.1525-142X.2003.03044.x","volume":"5","author":"AE Pasquinelli","year":"2003","unstructured":"Pasquinelli, A. E. et al. Expression of the 22 nucleotide let-7 heterochronic RNA throughout the Metazoa: a role in life history evolution? Evol Dev 5, 372\u2013378 (2003).","journal-title":"Evol Dev"},{"key":"BFsrep15683_CR6","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1016\/j.cell.2010.02.007","volume":"140","author":"SR Viswanathan","year":"2010","unstructured":"Viswanathan, S. R. & Daley, G. Q. Lin28: A microRNA regulator with a macro role. Cell 140, 445\u2013449 (2010).","journal-title":"Cell"},{"key":"BFsrep15683_CR7","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1016\/j.ceb.2009.04.009","volume":"21","author":"M Chekulaeva","year":"2009","unstructured":"Chekulaeva, M., Filipowicz, W. Mechanisms of miRNA-mediated post-transcriptional regulation in animal cells. Curr Opin Cell Biol 21, 452\u2013460 (2009).","journal-title":"Curr Opin Cell Biol"},{"key":"BFsrep15683_CR8","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","volume":"136","author":"DP Bartel","year":"2009","unstructured":"Bartel, D. P. MicroRNAs: target recognition and regulatory functions. Cell 136, 215\u2013233 (2009).","journal-title":"Cell"},{"key":"BFsrep15683_CR9","doi-asserted-by":"crossref","unstructured":"Leppert, U., Henke, W., Huang, X., Muller, J. M. & Dubiel, W. Post-Transcriptional Fine-tuning of COP9 Signalosome Subunit Biosynthesis is Regulated by the c-Myc\/Lin28B\/let-7 Pathway. J Mol Biol (2011).","DOI":"10.1016\/j.jmb.2011.04.041"},{"key":"BFsrep15683_CR10","doi-asserted-by":"crossref","unstructured":"Nadiminty, N. et al. MicroRNA let-7c suppresses androgen receptor expression and activity via regulation of Myc expression in prostate cancer cells. J Biol Chem (2011).","DOI":"10.1074\/jbc.M111.278705"},{"key":"BFsrep15683_CR11","doi-asserted-by":"publisher","first-page":"3384","DOI":"10.1073\/pnas.0808300106","volume":"106","author":"TC Chang","year":"2009","unstructured":"Chang, T. C. et al. Lin-28B transactivation is necessary for Myc-mediated let-7 repression and proliferation. Proc Natl Acad Sci USA 106, 3384\u20133389 (2009).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep15683_CR12","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1038\/emboj.2008.294","volume":"28","author":"S Dangi-Garimella","year":"2009","unstructured":"Dangi-Garimella, S. et al. Raf kinase inhibitory protein suppresses a metastasis signalling cascade involving LIN28 and let-7. EMBO J 28, 347\u2013358 (2009).","journal-title":"EMBO J"},{"key":"BFsrep15683_CR13","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1016\/j.tcb.2008.07.007","volume":"18","author":"S Roush","year":"2008","unstructured":"Roush, S. & Slack, F. J. The let-7 family of microRNAs. Trends Cell Biol 18, 505\u2013516 (2008).","journal-title":"Trends Cell Biol"},{"key":"BFsrep15683_CR14","first-page":"170","volume":"2","author":"M Sachdeva","year":"2010","unstructured":"Sachdeva, M. & Mo, Y. Y. miR-145-mediated suppression of cell growth, invasion and metastasis. Am J Transl Res 2, 170\u2013180 (2010).","journal-title":"Am J Transl Res"},{"key":"BFsrep15683_CR15","doi-asserted-by":"publisher","first-page":"3207","DOI":"10.1073\/pnas.0808042106","volume":"106","author":"M Sachdeva","year":"2009","unstructured":"Sachdeva, M. et al. p53 represses c-Myc through induction of the tumor suppressor miR-145. Proc Natl Acad Sci USA 106, 3207\u20133212 (2009).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep15683_CR16","doi-asserted-by":"publisher","first-page":"942","DOI":"10.1210\/en.2012-2006","volume":"154","author":"S Sangiao-Alvarellos","year":"2013","unstructured":"Sangiao-Alvarellos, S. et al. Changes in Hypothalamic Expression of the Lin28\/let-7 System and Related MicroRNAs During Postnatal Maturation and After Experimental Manipulations of Puberty. Endocrinology 154, 942\u2013955 (2013).","journal-title":"Endocrinology"},{"key":"BFsrep15683_CR17","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1038\/ng.125","volume":"40","author":"G Lettre","year":"2008","unstructured":"Lettre, G. et al. Identification of ten loci associated with height highlights new biological pathways in human growth. Nat Genet 40, 584\u2013591 (2008).","journal-title":"Nat Genet"},{"key":"BFsrep15683_CR18","doi-asserted-by":"publisher","first-page":"773","DOI":"10.1016\/j.ajhg.2010.03.010","volume":"86","author":"E Widen","year":"2010","unstructured":"Widen, E. et al. Distinct Variants at LIN28B Influence Growth in Height from Birth to Adulthood. Am J Hum Genet 86, 773\u2013782 (2010).","journal-title":"Am J Hum Genet"},{"key":"BFsrep15683_CR19","doi-asserted-by":"publisher","first-page":"3063","DOI":"10.1210\/jc.2009-2344","volume":"95","author":"J Tommiska","year":"2010","unstructured":"Tommiska, J. et al. LIN28B in Constitutional Delay of Growth and Puberty. J Clin Endocrinol Metab 95, 3063\u20133066 (2010).","journal-title":"J Clin Endocrinol Metab"},{"key":"BFsrep15683_CR20","doi-asserted-by":"publisher","first-page":"724","DOI":"10.1038\/ng.385","volume":"41","author":"C He","year":"2009","unstructured":"He, C. et al. Genome-wide association studies identify loci associated with age at menarche and age at natural menopause. Nat Genet 41, 724\u2013728 (2009).","journal-title":"Nat Genet"},{"key":"BFsrep15683_CR21","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1038\/ng.382","volume":"41","author":"KK Ong","year":"2009","unstructured":"Ong, K. K. et al. Genetic variation in LIN28B is associated with the timing of puberty. Nat Genet 41, 729\u2013733 (2009).","journal-title":"Nat Genet"},{"key":"BFsrep15683_CR22","doi-asserted-by":"publisher","first-page":"648","DOI":"10.1038\/ng.386","volume":"41","author":"JR Perry","year":"2009","unstructured":"Perry, J. R. et al. Meta-analysis of genome-wide association data identifies two loci influencing age at menarche. Nat Genet 41, 648\u2013650 (2009).","journal-title":"Nat Genet"},{"key":"BFsrep15683_CR23","doi-asserted-by":"publisher","first-page":"734","DOI":"10.1038\/ng.383","volume":"41","author":"P Sulem","year":"2009","unstructured":"Sulem, P. et al. Genome-wide association study identifies sequence variants on 6q21 associated with age at menarche. Nat Genet 41, 734\u2013738 (2009).","journal-title":"Nat Genet"},{"key":"BFsrep15683_CR24","doi-asserted-by":"publisher","first-page":"626","DOI":"10.1038\/ng.593","volume":"42","author":"H Zhu","year":"2010","unstructured":"Zhu, H. et al. Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies. Nat Genet 42, 626\u2013630 (2010).","journal-title":"Nat Genet"},{"key":"BFsrep15683_CR25","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.cell.2011.08.033","volume":"147","author":"H Zhu","year":"2011","unstructured":"Zhu, H. et al. The Lin28\/let-7 Axis Regulates Glucose Metabolism. Cell 147, 81\u201394 (2011).","journal-title":"Cell"},{"key":"BFsrep15683_CR26","doi-asserted-by":"publisher","first-page":"1563","DOI":"10.1002\/stem.1423","volume":"31","author":"G Shinoda","year":"2013","unstructured":"Shinoda, G. et al. Fetal deficiency of lin28 programs life-long aberrations in growth and glucose metabolism. Stem Cells 31, 1563\u20131573 (2013).","journal-title":"Stem Cells"},{"key":"BFsrep15683_CR27","doi-asserted-by":"crossref","unstructured":"Ojeda, S. R. & Skinner, M. K. Puberty in the rat. In: The Physiology of Reproduction (ed^(eds Neill JD ). Academic Press\/Elsevier, pp 2061\u20132126 (2006).","DOI":"10.1016\/B978-012515400-0\/50043-9"},{"key":"BFsrep15683_CR28","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1210\/er.2002-0019","volume":"24","author":"AS Parent","year":"2003","unstructured":"Parent, A. S. et al. The timing of normal puberty and the age limits of sexual precocity: variations around the world, secular trends and changes after migration. Endocr Rev 24, 668\u2013693 (2003).","journal-title":"Endocr Rev"},{"key":"BFsrep15683_CR29","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1159\/000262527","volume":"17","author":"SR Ojeda","year":"2010","unstructured":"Ojeda, S. R., Lomniczi, A., Sandau, U. & Matagne, V. New Concepts on the Control of the Onset of Puberty. Endocr Dev 17, 44\u201351 (2010).","journal-title":"Endocr Dev"},{"key":"BFsrep15683_CR30","doi-asserted-by":"publisher","first-page":"1552","DOI":"10.1016\/j.fertnstert.2014.04.025","volume":"101","author":"N Kotaja","year":"2014","unstructured":"Kotaja, N. MicroRNAs and spermatogenesis. Fertil Steril 101, 1552\u20131562 (2014).","journal-title":"Fertil Steril"},{"key":"BFsrep15683_CR31","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1530\/REP-08-0494","volume":"137","author":"Z He","year":"2009","unstructured":"He, Z., Kokkinaki, M., Pant, D., Gallicano, G. I. & Dym, M. Small R. N. A. molecules in the regulation of spermatogenesis. Reproduction 137, 901\u2013911 (2009).","journal-title":"Reproduction"},{"key":"BFsrep15683_CR32","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1007\/s10815-009-9305-y","volume":"26","author":"N Yan","year":"2009","unstructured":"Yan, N. et al. Microarray profiling of microRNAs expressed in testis tissues of developing primates. J Assist Reprod Genet 26, 179\u2013186 (2009).","journal-title":"J Assist Reprod Genet"},{"key":"BFsrep15683_CR33","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1186\/1471-213X-9-38","volume":"9","author":"K Zheng","year":"2009","unstructured":"Zheng, K., Wu, X., Kaestner, K. H. & Wang, P. J. The pluripotency factor LIN28 marks undifferentiated spermatogonia in mouse. BMC Dev Biol 9, 38 (2009).","journal-title":"BMC Dev Biol"},{"key":"BFsrep15683_CR34","doi-asserted-by":"publisher","first-page":"909","DOI":"10.1038\/nature08210","volume":"460","author":"JA West","year":"2009","unstructured":"West, J. A. et al. A role for Lin28 in primordial germ-cell development and germ-cell malignancy. Nature 460, 909\u2013913 (2009).","journal-title":"Nature"},{"key":"BFsrep15683_CR35","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1093\/molehr\/gas025","volume":"18","author":"N Aeckerle","year":"2012","unstructured":"Aeckerle, N. et al. The pluripotency factor LIN28 in monkey and human testes: a marker for spermatogonial stem cells? Mol Hum Reprod 18, 477\u2013488 (2012).","journal-title":"Mol Hum Reprod"},{"key":"BFsrep15683_CR36","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.1002\/stem.1343","volume":"31","author":"G Shinoda","year":"2013","unstructured":"Shinoda, G. et al. Lin28a regulates germ cell pool size and fertility. Stem Cells 31, 1001\u20131009 (2013).","journal-title":"Stem Cells"},{"key":"BFsrep15683_CR37","doi-asserted-by":"publisher","first-page":"860","DOI":"10.1002\/stem.1584","volume":"32","author":"P Chakraborty","year":"2014","unstructured":"Chakraborty, P. et al. LIN28A marks the spermatogonial progenitor population and regulates its cyclic expansion. Stem Cells 32, 860\u2013873 (2014).","journal-title":"Stem Cells"},{"key":"BFsrep15683_CR38","doi-asserted-by":"publisher","first-page":"5164","DOI":"10.1210\/en.2005-0455","volume":"146","author":"ML Barreiro","year":"2005","unstructured":"Barreiro, M. L. et al. Pattern of orexin expression and direct biological actions of orexin-a in rat testis. Endocrinology 146, 5164\u20135175 (2005).","journal-title":"Endocrinology"},{"key":"BFsrep15683_CR39","doi-asserted-by":"publisher","first-page":"2359","DOI":"10.1210\/en.2008-0580","volume":"150","author":"VM Navarro","year":"2009","unstructured":"Navarro, V. M. et al. Persistent impairment of hypothalamic KiSS-1 system after exposures to estrogenic compounds at critical periods of brain sex differentiation. Endocrinology 150, 2359\u20132367 (2009).","journal-title":"Endocrinology"},{"key":"BFsrep15683_CR40","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1677\/joe.0.1650345","volume":"165","author":"M Tena-Sempere","year":"2000","unstructured":"Tena-Sempere, M. et al. Neonatal exposure to estrogen differentially alters estrogen receptor alpha and beta mRNA expression in rat testis during postnatal development. J Endocrinol 165, 345\u2013357 (2000).","journal-title":"J Endocrinol"},{"key":"BFsrep15683_CR41","first-page":"671","volume":"3","author":"M Tena-Sempere","year":"2000","unstructured":"Tena-Sempere, M., Pinilla, L., Gonzalez, L. C. & Aguilar, E. Reproductive disruption by exposure to exogenous estrogenic compounds during sex differentiation: Lessons from the neonatally estrogenized male rat. Curr Top Steroid Res 3, 671\u2013678 (2000).","journal-title":"Curr Top Steroid Res"},{"key":"BFsrep15683_CR42","first-page":"208","volume":"117","author":"PC Sizonenko","year":"1985","unstructured":"Sizonenko, P. C., Lang, U., Rivest, R. W. & Aubert, M. L. The pineal and pubertal development. Ciba Found Symp 117, 208\u2013230 (1985).","journal-title":"Ciba Found Symp"},{"key":"BFsrep15683_CR43","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1159\/000122527","volume":"21","author":"JA Ramaley","year":"1976","unstructured":"Ramaley, J. A. Entrainment of the adrenal rhythm to photoperiod prior to puberty: effects of early experience on the adrenal rhythm and puberty. Neuroendocrinology 21, 225\u2013235 (1976).","journal-title":"Neuroendocrinology"},{"key":"BFsrep15683_CR44","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1111\/j.1600-079X.1988.tb00769.x","volume":"5","author":"CA Leadem","year":"1988","unstructured":"Leadem, C. A. Photoperiodic sensitivity of prepubertal female Fisher 344 rats. J Pineal Res 5, 63\u201370 (1988).","journal-title":"J Pineal Res"},{"key":"BFsrep15683_CR45","doi-asserted-by":"publisher","first-page":"3396","DOI":"10.1210\/en.2010-1415","volume":"152","author":"JM Castellano","year":"2011","unstructured":"Castellano, J. M. et al. Early metabolic programming of puberty onset: impact of changes in postnatal feeding and rearing conditions on the timing of puberty and development of the hypothalamic kisspeptin system. Endocrinology 152, 3396\u20133408 (2011).","journal-title":"Endocrinology"},{"key":"BFsrep15683_CR46","doi-asserted-by":"publisher","first-page":"3247","DOI":"10.1242\/jcs.01196","volume":"117","author":"R Nogueiras","year":"2004","unstructured":"Nogueiras, R. et al. Novel expression of resistin in rat testis: functional role and regulation by nutritional status and hormonal factors. J Cell Sci 117, 3247\u20133257 (2004).","journal-title":"J Cell Sci"},{"key":"BFsrep15683_CR47","doi-asserted-by":"publisher","first-page":"2297","DOI":"10.1210\/en.2003-1405","volume":"145","author":"ML Barreiro","year":"2004","unstructured":"Barreiro, M. L. et al. Orexin 1 receptor messenger ribonucleic acid expression and stimulation of testosterone secretion by orexin-A in rat testis. Endocrinology 145, 2297\u20132306 (2004).","journal-title":"Endocrinology"},{"key":"BFsrep15683_CR48","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1677\/joe.0.1190051","volume":"119","author":"HM Charlton","year":"1988","unstructured":"Charlton, H. M. et al. Growth hormone-deficient dwarfism in the rat: a new mutation. J Endocrinol 119, 51\u201358 (1988).","journal-title":"J Endocrinol"},{"key":"BFsrep15683_CR49","doi-asserted-by":"publisher","first-page":"1775","DOI":"10.1097\/00001756-200210070-00016","volume":"13","author":"M Lopez","year":"2002","unstructured":"Lopez, M., Seoane, L., Tovar, S., Senaris, R. M. & Dieguez, C. Thyroid status regulates CART but not AgRP mRNA levels in the rat hypothalamus. Neuroreport 13, 1775\u20131779 (2002).","journal-title":"Neuroreport"},{"key":"BFsrep15683_CR50","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.1038\/nm.2207","volume":"16","author":"M Lopez","year":"2010","unstructured":"Lopez, M. et al. Hypothalamic AMPK and fatty acid metabolism mediate thyroid regulation of energy balance. Nat Med 16, 1001\u20131008 (2010).","journal-title":"Nat Med"},{"key":"BFsrep15683_CR51","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/B978-0-12-387038-4.00001-X","volume":"99","author":"V Mondol","year":"2012","unstructured":"Mondol, V. & Pasquinelli, A. E. Let\u2019s make it happen: the role of let-7 microRNA in development. Curr Top Dev Biol 99, 1\u201330 (2012).","journal-title":"Curr Top Dev Biol"},{"key":"BFsrep15683_CR52","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1016\/j.ymeth.2010.07.002","volume":"52","author":"S Jorgensen","year":"2010","unstructured":"Jorgensen, S., Baker, A., Moller, S. & Nielsen, B. S. Robust one-day in situ hybridization protocol for detection of microRNAs in paraffin samples using LNA probes. Methods 52, 375\u201381 (2010).","journal-title":"Methods"},{"key":"BFsrep15683_CR53","doi-asserted-by":"publisher","first-page":"1321","DOI":"10.1210\/en.2012-1745","volume":"154","author":"F Gaytan","year":"2013","unstructured":"Gaytan, F. et al. Distinct expression patterns predict differential roles of the miRNA-binding proteins, Lin28 and Lin28b, in the mouse testis: studies during postnatal development and in a model of hypogonadotropic hypogonadism. Endocrinology 154, 1321\u20131336 (2013).","journal-title":"Endocrinology"},{"key":"BFsrep15683_CR54","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1002\/jemt.20783","volume":"73","author":"L Hermo","year":"2010","unstructured":"Hermo, L., Pelletier, R. M., Cyr, D. G. & Smith, C. E. Surfing the wave, cycle, life history and genes\/proteins expressed by testicular germ cells. Part 1: background to spermatogenesis, spermatogonia and spermatocytes. Microsc Res Tech 73, 241\u2013278 (2010).","journal-title":"Microsc Res Tech"},{"key":"BFsrep15683_CR55","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1095\/biolreprod.110.089458","volume":"85","author":"MH Tong","year":"2011","unstructured":"Tong, M. H., Mitchell, D., Evanoff, R. & Griswold, M. D. Expression of Mirlet7 Family MicroRNAs in Response to Retinoic Acid-Induced Spermatogonial Differentiation in Mice. Biol Reprod 85, 189\u2013197 (2011).","journal-title":"Biol Reprod"},{"key":"BFsrep15683_CR56","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1161\/CIRCRESAHA.109.197517","volume":"105","author":"Y Cheng","year":"2009","unstructured":"Cheng, Y. et al. MicroRNA-145, a novel smooth muscle cell phenotypic marker and modulator, controls vascular neointimal lesion formation. Circ Res 105, 158\u2013166 (2009).","journal-title":"Circ Res"},{"key":"BFsrep15683_CR57","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1038\/nature08195","volume":"460","author":"KR Cordes","year":"2009","unstructured":"Cordes, K. R. et al. miR-145 and miR-143 regulate smooth muscle cell fate and plasticity. Nature 460, 705\u2013710 (2009).","journal-title":"Nature"},{"key":"BFsrep15683_CR58","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/S0378-4320(97)00019-5","volume":"49","author":"CG Gravance","year":"1997","unstructured":"Gravance, C. G., Breier, B. H., Vickers, M. H. & Casey, P. J. Impaired sperm characteristics in postpubertal growth-hormone-deficient dwarf (dw\/dw) rats. Anim Reprod Sci 49, 71\u201376 (1997).","journal-title":"Anim Reprod Sci"},{"key":"BFsrep15683_CR59","doi-asserted-by":"publisher","first-page":"1681","DOI":"10.1210\/endo.140.4.6661","volume":"140","author":"M Kanzaki","year":"1999","unstructured":"Kanzaki, M. & Morris, P. L. Growth hormone regulates steroidogenic acute regulatory protein expression and steroidogenesis in Leydig cell progenitors. Endocrinology 140, 1681\u20131686 (1999).","journal-title":"Endocrinology"},{"key":"BFsrep15683_CR60","doi-asserted-by":"publisher","first-page":"4061","DOI":"10.1210\/endo.137.9.8756586","volume":"137","author":"BH Breier","year":"1996","unstructured":"Breier, B. H., Vickers, M. H., Gravance, C. G. & Casey, P. J. Growth hormone (GH) therapy markedly increases the motility of spermatozoa and the concentration of insulin-like growth factor-I in seminal vesicle fluid in the male GH-deficient dwarf rat. Endocrinology 137, 4061\u20134064 (1996).","journal-title":"Endocrinology"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep15683.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep15683","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep15683.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T08:47:22Z","timestamp":1672908442000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep15683"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,23]]},"references-count":60,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2015,12,21]]}},"alternative-id":["BFsrep15683"],"URL":"https:\/\/doi.org\/10.1038\/srep15683","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10,23]]},"assertion":[{"value":"14 June 2015","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 September 2015","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 October 2015","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"15683"}}