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The purpose of this study was to investigate whether a Microbial Ecosystem Therapeutic (MET-1) consisting of 33 bacterial strains, isolated from human stool and previously used to cure patients with recurrent <jats:italic>Clostridium difficile<\/jats:italic> infection, could also protect against <jats:italic>S. typhimurium<\/jats:italic> disease. C57BL\/6\u2009mice were pretreated with streptomycin prior to receiving MET-1 or control, then gavaged with <jats:italic>S. typhimurium.<\/jats:italic> Weight loss, serum cytokine levels and <jats:italic>S. typhimurium<\/jats:italic> splenic translocation were measured. NF-\u03baB nuclear staining, neutrophil accumulation and localization of tight junction proteins (claudin-1, ZO-1) were visualized by immunofluorescence. Infected mice receiving MET-1 lost less weight, had reduced serum cytokines, reduced NF-\u03baB nuclear staining and decreased neutrophil infiltration in the cecum. MET-1 also preserved cecum tight junction protein expression and reduced <jats:italic>S. typhimurium<\/jats:italic> translocation to the spleen. Notably, MET-1 did not decrease CFUs of <jats:italic>Salmonella<\/jats:italic> in the intestine. MET-1 may attenuate systemic infection by preserving tight junctions, thereby inhibiting <jats:italic>S<\/jats:italic>. <jats:italic>typhimurium<\/jats:italic> from gaining access to the systemic circulation. We conclude that MET-1 may be protective against enteric infections besides <jats:italic>C. difficile<\/jats:italic> infection.<\/jats:p>","DOI":"10.1038\/srep16094","type":"journal-article","created":{"date-parts":[[2015,11,4]],"date-time":"2015-11-04T10:00:46Z","timestamp":1446631246000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Administration of defined microbiota is protective in a murine Salmonella infection model"],"prefix":"10.1038","volume":"5","author":[{"given":"Sarah-Lynn E.","family":"Martz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie A. 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